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Acute Toxicological Effects of Ingested Tooth Whiteners in Female Rats

Department of Community Dentistry

D.E. Bowers, Jr

Department of Biomedical Sciences

L. Thomas

Department of Pediatric Dentistry, School of Dental Medicine, Southern Illinois University-Edwardsville, 2800 College Avenue, Alton, Illinois 62002-4798

A.F. Redmond

Department of Biomedical Sciences

Tooth whiteners are considered as cosmetic agents to be used for bleaching teeth. Since tooth whitener may be swallowed during the whitening procedure, studies were conducted to determine whether ingestion of tooth whitener containing carbamide peroxide resulted in toxic effects. Adult female rats were used, and vaginal smears were examined daily to determine whether the animals were ovulating. Following an overnight fast, a single bolus of a commercial tooth whitener (5 g of tooth whitener/kg fasting body weight) was administered by gavage. Control rats received de-ionized water. After 2 h, mean respirations per min of animals receiving the tooth whitener Quik Start (contains 35% carbamide peroxide) decreased from 169 to 55, and body temperature decreased from 38.4 to 34°C. Other distress signs included: labored breathing, loss of righting reflex, partial eye closure, bloody urine, and incontinence. Three of 22 animals (3/22) died within 48 h, of gastric hemorrhaging. Eight/10 rats stopped ovulating. At necropsy 2 weeks post-dosing, 10/19 animals had grossly bloated stomachs, and mucosal necrosis was observed histologically in 3. Animals receiving White & Brite or Nu-Smile (containing 10 or 15% carbamide peroxide, respectively) exhibited similar but milder symptoms. The data indicate that ingestion of large doses of commercial preparations of tooth whiteners may be acutely toxic, sometimes fatal, to female laboratory rats.

REFERENCES

• Armstrong DT (1986). Environmental stress and ovarian function. Biol Reprod 34:29-39.[Abstract]
• Babior BM (1984). Oxidants from phagocytes: agents of defense and destruction. Blood 64:959-966.[Free Full Text]
• Bowles WH, Thompson LR (1986). Vital bleaching: The effects of heat and hydrogen peroxide on pulpal enzymes. J Endod 12:108-112.[CrossRef][Medline] [Order article via Infotrieve]
• Chance B., Sies H., Boveris A. (1979). Hydroperoxide metabolism in mammalian organs. Physiol Rev 59:527-605.[Free Full Text]
• Christensen DW, Faught WE, Black RE, Woodward GA, Timmons OD (1992). Fatal oxygen embolization after hydrogen peroxide ingestion. Crit Care Med 20:543-544.[Medline] [Order article via Infotrieve]
• Christensen GJ, editor (1989a). Subject: tooth bleaching, home-use products. 1. Background. Clin Res Assoc Newsletter 13(7):1-2.
• Christensen GJ, editor (1989b). In vivo assays for presence of gel in trays. 1.F. Answers to commonly asked questions. Clin Res Assoc Newsletter 13(12):2.
• Davis WL, Jacoby BH, Craig KR, Wagner G., Harrison JW (1991). Copper-zinc superoxide dismutase activity in normal and inflamed human dental pulp tissue. J Endod 17:316-318.[Medline] [Order article via Infotrieve]
• Dorman HL, Bishop JG (1970). Production of experimental edema in dog tongue with dilute hydrogen peroxide. Oral Surg 29:38-43.[CrossRef][Medline] [Order article via Infotrieve]
• Edwall L., Olgart LG (19721. Influence of cavity washing agents on pulpal microcirculation in the cat. Acta Odontol Scand 30:39-47.
• Fantone JC, Ward PA ( 1982). Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 107:397-418.
• Fat JC, Corcoran JF, Hanks CT (1991). Cytotoxicity and dentin permeability of "Night Guard" vital bleaching material in vitro. J Endod 17:193.
• Federal Register (1985). Acute oral toxicity. 50:39402-39404.
• Feinman RA, Madray G., Yarborough D. (1991). Chemical, optical, and physiologic mechanism of bleaching products: a review. Pract Periodontics Aesthet Dent 3:32-37.[Medline] [Order article via Infotrieve]
• Freeman BA, Crapo JD (1982). Biology of disease. Free radicals and tissue injury. Lab Invest 47:412-426.[Medline] [Order article via Infotrieve]
• Fridovich I. (1979), The biology of oxygen radicals. Science 201:875-880.[CrossRef]
• Gage TW, Frazier LW, Wright JM (1992). Oral soft tissue response to topically applied peroxide tooth whitener (abstract). J Dent Res 71 (Spec Iss):585.
• Garber DA Goldstein CE, Goldstein RE, Schwartz CG (1991). Dentist-monitored bleaching. Pract Periodontics Aesthet Dent 3:22-26.[Medline] [Order article via Infotrieve]
• Gilberson TP, Kern JD, Pettigrew DW, Eaves CC, Haynes JF (1989). Near fatal hydrogen peroxide ingestion. Ann Emerg Med 18:778-779.[CrossRef][Medline] [Order article via Infotrieve]
• Giusti GV (1973). Fatal poisoning with hydrogen peroxide. Forensic Sci 2:99-100.[CrossRef][Medline] [Order article via Infotrieve]
• Haywood VB, Heymann HO (1989). Night guard bleaching. Quint Int 20:173-176.
• Humberston CL, Dean BS, Krenzelok EP (1990). Ingestion of 35% hydrogen peroxide. J Toxicol Clin Toxicol 28:95-100.[Medline] [Order article via Infotrieve]
• Kamel F., Kubajak CL (1987). Modulation of gonadotropin secretion by corticosterone: interaction with gonadal steroids and mechanism of action. Endocrinology 121:561-568.[Abstract/Free Full Text]
• Martin JH, Bishop JG, Guentherman RH, Dorman HL (1968). Cellular response of gingiva to prolonged application of dilute hydrogen peroxide. J Periodontol 39:208-210.[Medline] [Order article via Infotrieve]
• Mayes PA (1990). Biological oxidation. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper's biochemistry. Norwalk (CT): Appleton & Lange, 105-111.
• Miller MB, Mabrito CA, editors (1989). White & Brite. Reality Now 4:1-2.
• Osol A, Pratt R, Altschule MD, editors (1967). The United States dispensatory and physicians' pharmacology. 26th ed. Hydrogen peroxide solution. Philadelphia: Lippincott Co., 583.
• Rackoff WR, Merton DF (1990). Gas embolism after ingestion of hydrogen peroxide. Pediatrics 85:593-594.[Abstract/Free Full Text]
• Rees TD, Orth CF ( 1986). Oral ulcerations with use of hydrogen peroxide. J Periodontol 57:689-692.[Medline] [Order article via Infotrieve]
• Rivier C., Vale W. ( 1984). Influence of corticotropin-releasing factor on reproductive functions in the rat. Endocrinology 114:914-921.[Abstract/Free Full Text]
• Rodriguez-Echandia EL, Gonzalez AS, Cabrera R., Fracchia LN (1988). A further analysis of behavioral and endocrine effects of unpredictable chronic stress. Physiol & Behau 43:789-795.[CrossRef]
• Rodwell VW (1990). Catabolism of proteins and of amino acid nitrogen. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper's biochemistry. Norwalk (CT Appleton & Lange, 273-283.
• Seale NS, Mclntosh JE, Taylor AN (1981). Pulpal reaction to bleaching of teeth in dogs. J Dent Res 60:948-953.[Abstract/Free Full Text]
• Sigma Chemical Company (1993). Biochemicals, organic compounds and diagnostic reagents. St. Louis (MO): 1000.
• Viau V., Meaney MJ (1991). Variations in the hypothalamic-pituitary-adrenal response to stress during the estrous cycle in the rat. Endocrinology 129:2503-2511.[Abstract/Free Full Text]
• Yarborough DK (1991). The safety and efficacy of tooth bleaching: a review of the literature 1988-1990. Compend Contin Educ Dent XII:191-196.

Journal of Dental Research, Vol. 72, No. 9, 1298-1303 (1993)
DOI: 10.1177/00220345930720090701


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