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The Effects of Titanium and Hydroxyapatite on Osteoblastic Expression and Proliferation in Rat Parietal Bone Cultures

Department of Oral Biology, Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 69978, Israel

S. Pitaru

Department of Oral Biology, Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 69978, Israel

M.M. Weinreb

Department of Oral Biology, Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 69978, Israel

Titanium and hydroxyapatite are used for the fabrication of dental and orthopedic implants. The longevity of these implants depends on the amount and rate of bone formation that occurs around their surfaces. In the present study, the effects of titanium, hydroxyapatite, and polystyrene (control) on the proliferation of rat calvarial cells, and on their capacity to express alkaline phosphatase and respond to parathyroid hormone (PTH) stimulation, were studied. The nature of the substrate did not affect the DNA and protein contents of experimental and control cultures throughout the experimental period. Alkaline phosphatase expression and PTH response, as assessed by DNA synthesis and adenylate cyclase activity, were higher in cultures grown on hydroxyapatite and polystyrene than in those grown on titanium. These results indicate that hydroxyapatite was a more favorable substrate than titanium for the growth and differentiation of osteoblast-like cells in vitro.

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Journal of Dental Research, Vol. 72, No. 6, 1005-1008 (1993)
DOI: 10.1177/00220345930720060201


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Massas, R. Articles by Weinreb, M.M. Search for Related Content PubMed PubMed Citation Articles by Massas, R. Articles by Weinreb, M.M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS HYDROXYAPATITE PARATHYROID HORMONE TITANIUM Social Bookmarking                         What's this?