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A Study of Macrophages, Macrophage-related Cells, and Endothelial Adhesion Molecules in Recurrent Aphthous Ulcers in HIV-positive Patients

Department of Stomatology, School of Dentistry, Division of Oral Pathology, University of California, San Francisco, California 94143-0424

L.A. Macphail

Department of Stomatology, School of Dentistry, Division of Oral Pathology, University of California, San Francisco, California 94143-0424

D.W. Richards

Department of Stomatology, School of Dentistry, Division of Oral Pathology, University of California, San Francisco, California 94143-0424

J.S. Greenspan

Department of Stomatology, School of Dentistry, Division of Oral Pathology, University of California, San Francisco, California 94143-0424

The purpose of this immunohistochemical study was to investigate the presence and distribution of macrophages (CDllc+ and CD68+) and macrophage-related dendritic cells (factor XIIIa+ and CD36+) in early and late aphthous ulcers associated with HIV infection. To substantiate a mechanism by which these cells may move from the vascular compartment to tissue spaces, we also investigated expression of ELAM (endothelial leukocyte adhesion molecule), ICAM-1 (intercellular adhesion molecule), and CD18 (leukocyte function antigen). Numerous CD 1 lc+ and CD68+ macrophages were seen in early lesions, though larger numbers of CD68+ cells were present in older lesions. No significant increases in factor XIIIa+ dendrocytes were seen in either early or late lesions, though dendrocytes appeared enlarged. CD36+ cells and CD18+ leukocytes were more numerous in early than in late aphthous ulcers. ELAM and ICAM expression was most intense on endothelial cells in early aphthous ulcers, with staining intensity fading toward the lesion periphery. Control specimens showed weaker ELAM and ICAM staining than did the ulcer specimens. Keratinocytes did not express ICAM. By virtue of their numbers, macrophages and macrophage subtypes appear to have a significant role in both the early and late stages of this disease. Although factor XIIIa-expressing dendrocytes may not have been more numerous in the ulcers, they appeared to be "activated" because of their prominence in the lesions and their occasional co-expression of CD68 antigen (KP1+). They may have a minor role in antigen processing, phagocytosis, and fibroplasia. ELAM and ICAM expression by endothelial cells provides a mechanism by which macrophages and other leukocytes can be recruited to the site of the lesion. This mechanism appears to be functional in HIV-positive patients.

REFERENCES

• Cerio R., Griffiths C., Cooper K., Nickoloff B., Headington J. (1989). Characterization of factor XIIIa positive dermal dendritic cells in normal and inflamed skin. Br J Dermatol 121:421-431.[CrossRef][Medline] [Order article via Infotrieve]
• Cerio R., Spaull J., Oliver G., Wilpon-Jones E. (1990), A study of factor XIIIa and MAC 387 immunolabeling in normal and pathological skin. Am J Dermatopathol 12:221-233.[Medline] [Order article via Infotrieve]
• Greenspan D., Greenspan JS, Schiødt M., Pindborg JJ (1990). AIDS and the mouth. Copenhagen; Munksgaard, 148-149.
• Greenspan JS, Gadol N., Olson JA, Hoover CI, Jacobsen PL, Shillitoe EJ (1985). Lymphocyte function in recurrent aphthous ulceration. J Oral Pathol 14:592-602.[CrossRef][Medline] [Order article via Infotrieve]
• Groves R., Allen M., Barker J., Haskard D. (1991). Endothelial leukocyte adhesion molecule-1 (ELAM-1) expression in cutaneous inflammation. Br J Dermatol 124:117-123.[CrossRef][Medline] [Order article via Infotrieve]
• Hayrinen-Immonen R., Malmström M., Nordström D., Sorsa T., Konttinen YT (1992). Distribution of adhesion in recurrent oral ulcers. J Oral Pathol Med 21:199-202.[CrossRef][Medline] [Order article via Infotrieve]
• Hunyadi J., Simon M. (1986). Expression of OKM5 antigen on human keratinocytes in vitro upon stimulation with gamma-interferon. Acta Derm Venereol (Stockh) 66:527-530.[Medline] [Order article via Infotrieve]
• Konter U., Kellner I., Hoffmeister B., Sterry W. (1990). Induction and upregulation of adhesion receptors in oral and dermal lichen planus. J Oral Pathol Med 19:459-463.[CrossRef][Medline] [Order article via Infotrieve]
• MacPhail LA, Greenspan D., Feigal DW, Lennette ET, Greenspan JS (1991a). Recurrent aphthous ulcers in association with HIV infection. Oral Surg Oral Med Oral Pathol 71:678-683.[CrossRef][Medline] [Order article via Infotrieve]
• MacPhail LA, Greenspan D., Katz MH, Dodd CL, Wong EL, Greenspan JS (1991b). Recurrent aphthous ulcers in HIV-infection: T-lymphocyte subsets (abstract). J Dent Res 70:331.
• Micklem K., Ringley E., Cordell J., Simmons D., Stross P., Turley H., et al. (1989). A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br J Haematol 73:6-11.[Medline] [Order article via Infotrieve]
• Nickoloff B., Griffiths C., Barker J. (1990). The role of adhesion molecules, chemotactic factors, and cytokines in inflammatory and neoplastic skin disease. J Invest Dermatol 94:151S- 157S.[CrossRef][Medline] [Order article via Infotrieve]
• Pedersen A., Hougen HP, Kenrad B. (1992). T-lymphocyte subsets in oral mucosa of patients with recurrent aphthous ulceration. J Oral Pathol Med 21:1760-1780.
• Penneys NS (1990). Factor XIII expression in the skin: observations and a hypothesis. J Am Acad Dermatol 22:484-488.[Medline] [Order article via Infotrieve]
• Phelan JA, Eisig S, Freedman PD, Newsome N, Klein RS (1991). Major aphthous-like ulcers in patients with AIDS. Oral Surg Oral Med Oral Pathol 71:68-72.[CrossRef][Medline] [Order article via Infotrieve]
• Pimpinelli N., Borgognoni L., Riccardi R., Ficarra G., Gaglioti D., Romagnoli P. (1991). CD36 (OKM5)+ dendritic cells in the oral mucosa of HIV- and HIV+ subjects. J Invest Dermatol 97:537-542.[CrossRef][Medline] [Order article via Infotrieve]
• Regezi JA, Nickoloff BJ, Headington JT (1992). Oral submucosal dendrocytes: factor XIIIa and CD34 dendritic cell populations in normal tissue and fibrovascular lesions. J Cutan Pathol 19:398-406.[CrossRef][Medline] [Order article via Infotrieve]
• Reid M., Gray C., Fear J., Bird C. ( 1986). Immunohistological demonstration of factors XIIIa and XIIIs in reactive and neoplastic fibroblastic and fibro-histiocytic lesions. Histopathology 10:1171-1178.[CrossRef][Medline] [Order article via Infotrieve]
• Savage NW, Seymour GJ, Kruger BJ (1985). T-lymphocyte subset changes in recurrent aphthous stomatitis. Oral Surg Oral Med Oral Pathol 60:175-181.[CrossRef][Medline] [Order article via Infotrieve]
• Savage NW, Seymour GJ, Kruger BJ (1986). Expression of class I and class II major histocompatibility complex antigens on epithelial cells in recurrent aphthous stomatitis. J Oral Pathol 15:191-195.[CrossRef][Medline] [Order article via Infotrieve]
• Schwarting R., Stein H., Wang CY ( 1985). The monoclonal antibodies S-HCL 1 (Leu-14) and S-HCL 3 (Leu-M5) allow the diagnosis of hairy cell leukemia. Blood 65:974-983.[Abstract/Free Full Text]
• Simon M., Hunyadi J. (1987). Expression of OKM5 antigen on human keratinocytes in positive intracutaneous tests for delayed-type hypersensitivity. Dermatologica 175:121-125.[Medline] [Order article via Infotrieve]
• Toida M., Watanabe F., Tsai CS, Okatomi T., Tatematsu N., Oka N. (1989). Factor XIIIa-containing cells and fibrosis in oral and maxillofacial lesions: an immunohistochemical study. Oral Surg Oral Med Oral Pathol 68:293-299.[CrossRef][Medline] [Order article via Infotrieve]
• Verdickt GM, Savage NW, Dodd NM, Walsh LJ (1992). Expression of the CD54 (ICAM-1) and CD11a (LFA-1) adhesion molecules in oral mucosal inflammation. J Oral Pathol Med 21:65-69.[CrossRef][Medline] [Order article via Infotrieve]
• Wood G., Turner R., Shiurba R., Eng L., Warnke R. (1985). Human dendritic cells and macrophages. Am J Pathol 119:73-82.[Abstract]

Journal of Dental Research, Vol. 72, No. 12, 1549-1553 (1993)
DOI: 10.1177/00220345930720120301


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