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Journal of Dental Research
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Immunolocalization of Integrin {alpha}6β4 in Mouse Junctional Epithelium Suggests an Anchoring Function to both the Internal and the External Basal Lamina

M. Hormia

Department of Periodontology, University of Helsinki, Mannerheimintie 172, SF-00300 Helsinki, Finland

I. Virtanen

Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, SF-00170 Helsinki, Finland

V. Quaranta

The Scripps Research Institute, Department of Immunology, 10666 North Torrey Pines Road, La Jolla, California 92037

The localization of the integrin {alpha} 6β4, a transmembrane adhesion molecule associated with hemidesmosomes, was studied in mouse junctional epithelium (JE) by the use of monoclonal antibodies in indirect immunofluorescence microscopy. The results showed that the integrin a6 subunit was expressed throughout the JE and was localized to the cell membranes, including the aspects facing the internal and external basal laminae. The β4 subunit had a more restricted distribution. It was expressed only in cells facing the internal and the external basal laminae and had a basally polarized distribution. In other parts of gingival epithelium, both integrin subunits were mainly expressed at the basal aspects of basal epithelial cells. The basement membrane components, type IV collagen and laminin, could be detected only in the external basal lamina and in other basement membranes of gingival epithelium. The results indicate that the a6β4 integrin, expressed in mouse JE, has a role in mediating the attachment of the cells to the basement membranes facing the connective tissue and the tooth.

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Journal of Dental Research, Vol. 71, No. 8, 1503-1508 (1992)
DOI: 10.1177/00220345920710080801
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