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Examination by X-ray Photoelectron Spectroscopy of the Adsorption of Chlorhexidine on Hydroxyapatite
R.N.S. Sodhi
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, Ontario, Canada M5S 1A1
H.A. Grad
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, Ontario, Canada M5S 1A1
D.C. Smith
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, Ontario, Canada M5S 1A1
X-ray photoelectron spectroscopy (XPS) was used for determination of the effects of chlorhexidine (CHX) solutions (0.2% and 1% solutions of the digluconate salt) on the elemental composition of hydroxyapatite surfaces. So that the nature of the adsorbed species after they were washed with water could be identified, comparisons were made with reference spectra for CHX obtained from a CHX digluconate film and CHX dichloride powder. The XPS results clearly indicated the retention of CHX moieties, which could be ascertained from the spectra by the presence of N and Cl, features unique to CHX. The spectral envelopes were virtually identical to those obtained from the reference spectra. High-resolution C Is spectra also gave support for the retention of CHX; however, the spectra differed from those of the CHX digluconate film in that no feature attributable to the C-OH of the gluconate anion was present, consistent with the view that the CHX cation remains behind to form an electrostatic bond with the phosphate groups of the hydroxyapatite. The N:Cl ratio for the washed samples was found to be higher than that for the reference samples and may be indicative of partial decomposition of the CHX. Decomposition was also seen to be induced by x-ray exposure. While the high-resolution spectra presented here do not directly address the controversy on the mechanism for the anti-plaque efficacy of CHX, they do provide the necessary basis for the application ofXPS to future in vitro studies on the retention of CHX to dental surfaces.
REFERENCES
- Andrade JD (1985). X-ray photoelectron spectroscopy. In: Andrade JD, editor. Surface and interfacial aspects of biomedical polymers. Vol. 1. New York: Plenum Press, 105-195.
- Anthony MT, Seah MP ( 1984). XPS: energy calibration of electron spectrometers. 1-An absolute, traceable energy calibration and the provision of atomic reference line energies. Surf Interf Anal 6:95-106.[CrossRef]
- AshleyJC (1980). Inelastic interactions oflow-energy electrons with organic solids: simple formulae. IEEE Trans Nucl Sci NS-27:1454-1458.
- Berresheim K., Mattern-Klossen M., Wilmers M. (1991). A standard form of spectra for quantitative ESCA-analysis. Fresenius J Anal Chem 341:121-124.[CrossRef]
- Briggs D, Seah MP, editors (1990). Practical surface analysis. 2nd ed. Vol. 1. Chichester (England): John Wiley and Sons.
- Busscher HJ, van der Mei HC, Genet MJ, Perdok JF, Rouxhet PG (1990). XPS determination ofthe thickness of adsorbed mouthrinse components on dental enamel. Surf Interf Anal 15:344-346.[CrossRef]
- Emilson CG, Ericson TH, Heyden G., Magnusson BC (1973). Uptake of chlorhexidine to hydroxyapatite. J Periodont Res 8 (Suppl 12):17-21. Gjermo P.( 1989). Chlorhexidine and related compounds. J Dent Res 68 (Spec Iss):1602-1608.
- Gjermo P., Bonesvoll P., Hjeljord LG, Rolla G. (1975). Influence of variation of pH of chlorhexidine mouth rinses on oral retention and plaque-inhibiting effect. Caries Res 9:74-82.[Medline]
[Order article via Infotrieve]
- Jenkins S., Addy M., Wade W. ( 1988). The mechanism of action of chlorhexidine. A study of plaque growth on enamel inserts in vivo. J Clin Periodontol 15:415-424.[CrossRef][Medline]
[Order article via Infotrieve]
- Kohlbecker G. (1989). Toxische verunreinigungen in chlorhexidin-digluconat. Dtsch Zahnärztl Z 44:273-276.[Medline]
[Order article via Infotrieve]
- Lemons JE (1990). Dental implant biomaterials. JAm DentAssoc 121:716-719.
- Nordbo H. (1972). The affinity of chlorhexidine for hydroxyapatite and tooth surfaces. Scand J Dent Res 80:465-473.[Medline]
[Order article via Infotrieve]
- Perdok JF, van der Mei HC, Genet MJ, Rouxhet PG, Busscher HJ (1989). Elemental surface concentration ratios and surface free energies of human enamel after application of chlorhexidine and adsorption of salivary constituents. Caries Res 23:297-302.[Medline]
[Order article via Infotrieve]
- Rolla G., Loë H., Schiøtt CR (1970). The affinity of chlorhexidine for hydroxyapatite and salivary mucins. J Periodont Res 5:90-95.[CrossRef][Medline]
[Order article via Infotrieve]
- van Attekum Pmt, Trooster JM (1977). Removal of x-ray satellites from Mg K
 excited photoelectron spectra. J Electron Spectrosc Relat Phenom 11:363-370.[CrossRef] - Zhou X.-L, White JM (1990). Photon- and electron-induced chemistry of chlorobenzene on Ag(111). J Chem Phys 92:5612-5621.[CrossRef]
Journal of Dental Research, Vol. 71, No. 8,
1493-1497 (1992)
DOI: 10.1177/00220345920710080601
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Surface Spectroscopy of Apatitic Materials: Limitations and Concerns
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November 1, 1996;
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1865 - 1870.
[Abstract]
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