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Arachidonic Acid Regulates the Phosphoinositide Signal Transduction Pathway in Submandibular Acinar Cells

Department of Oral Biology, Faculty of Dentistry, The University of Manitoba, 780 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E OW2

N. Fleming

Department of Oral Biology, Faculty of Dentistry, The University of Manitoba, 780 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E OW2

Modulation of the phosphoinositide signal transduction pathway by arachidonic acid (AA) in collagenase-dispersed rat submandibular acinar cells was investigated. The muscarinic agonist, carbachol, stimulated PIP₂ hydrolysis and the generation of IP ₃ to five-fold the control levels. This response was inhibited by 75% on pre-treatment of cells with AA. The AA inhibitory effect was not duplicated by a range of prostaglandins and leukotrienes and was not reversed by blockers of the cyclo-oxygenase and lipoxygenase synthetic pathways, indicating that AA action was not mediated by eicosanoid metabolites. Additional experiments confirmed that the enzyme, protein kinase C, was also not a mediator of the AA effect. Arachidonic acid did not affect the uptake of radioactive inositol into acinar cells, but it did inhibit the incorporation of inositol into inositol phospholipids of the phosphoinositide cycle. In studies on inositol phospholipid turnover, AA alone reduced the level of PIP₂ but not of PIP or PI. Under conditions of PI cycle stimulation with carbachol, AA significantly lowered PIP and PIP but not PI. These findings suggest that arachidonic acid may regulate the phosphoinositide response by inhibiting the synthetic phase of the cycle at a locus distal to PI generation.

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Journal of Dental Research, Vol. 71, No. 8, 1462-1467 (1992)
DOI: 10.1177/00220345920710080101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Chung, H.C. Articles by Fleming, N. Search for Related Content PubMed PubMed Citation Articles by Chung, H.C. Articles by Fleming, N. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CARBACHOL CHLORIDE Social Bookmarking                         What's this?