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The Role of Albumin in Developing Rodent Dental Enamel: A Possible Explanation for White Spot Hypoplasia

Department of Oral Biology, University of Leeds, Clarendon Way, Leeds, LS2 9LU, United Kingdom

J. Kirkham

Department of Oral Biology, University of Leeds, Clarendon Way, Leeds, LS2 9LU, United Kingdom

S.J. Brookes

Department of Oral Biology, University of Leeds, Clarendon Way, Leeds, LS2 9LU, United Kingdom

R.C. Shore

Department of Oral Biology, University of Leeds, Clarendon Way, Leeds, LS2 9LU, United Kingdom

The uptake of serum albumin by maturation-stage rodent enamel and the resulting effects on the growth of enamel crystallites were investigated in vitro. Albumin uptake was demonstrated by means of gel electrophoresis and confirmed by Western blotting with use of monoclonal antibodies. Measurement of crystal size was carried out by direct TEM measurement of enamel crystallite outlines after incubations in metastable solutions of calcium phosphate. The ability of endogenous enamel enzymes to degrade albumin was investigated by substrate-specific zymography. The results showed that albumin could be taken up by maturation-stage enamel and produce inhibition of crystallite growth. There was no detectable proteolytic activity in the enamel against albumin substrate, which suggests that albumin entering enamel by extravasation in vivo may produce incomplete tissue maturation, resulting in a white, opaque appearance on eruption.

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Journal of Dental Research, Vol. 71, No. 6, 1270-1274 (1992)
DOI: 10.1177/00220345920710060101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Robinson, C. Articles by Shore, R.C. Search for Related Content PubMed PubMed Citation Articles by Robinson, C. Articles by Shore, R.C. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS HYDROXYAPATITE Social Bookmarking                         What's this?