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An Immunohistochemical Study of the Distribution of Immunocompetent Cells, Especially Macrophages and Ia Antigen-expressing Cells of Heterogeneous Populations, in Normal Rat Molar Pulp
T. Okiji
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
N. Kawashima
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
T. Kosaka
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
A. Matsumoto
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
C. Kobayashi
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
H. Suda
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan
The precise distribution of various immunocompetent cells in rat molar pulp was immunohistochemically examined by use of seven anti-rat monoclonal antibodies. It was demonstrated that rat molar pulp contained many 0X6 (anti-la antigen-positive cells and a large number of ED1 (anti-monocytes, macrophages, and dendritic cells)-positive, ED2 (anti-tissue macrophages)-positive, and/or OX35 (anti-macrophages and CD4+ lymphocytes)-positive cells, Macrophage-like cells predominated in the central portion of the pulp, while cells of dendritic appearance usually existed in the periphery of the pulp. Double-immunaperoxidase staining revealed that these cells showed some heterogeneity, but the majority could be classified as ED1+/0X6-/ED2+ cells, which may be Ia histiocytes. Findings also suggested that true dendritic cells may be included in the ED1+/0X6+/ED2- category of cells. A small number of T lymphocytes and plasma cells were also detected. These results suggest that the normal dental pulp contains a variety of immunocompetent cells, with macrophages as the most dominating. Following the exogenous invasion of pathogenic stimuli in the pulp, these cells may participate in the defense reaction by acting as phagocytes or antigen-presenting cells, which are essential for the initiation of immune responses.
REFERENCES
- Avery JK (1976). Pulp. In: Bhaskar SN, editor. Orban's oral histology and embryology. 8th ed. St. Louis (MO): Mosby, 141-181.
- Barclay AN (1980). The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 54:589-599.
- Beelen Rhj, Eestermans IL, Dopp EA, Dijkstra CD (1987). Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: expression of recognized antigens in different stages of differentiation. Transplant Proc 3:3166-3170.
- Bergenholtz G. (1990). Pathogenic mechanisms in pulpal disease. J Endodont 16:98-101.[Medline]
[Order article via Infotrieve]
- Bergenholtz G., Nagaoka S., Jontell M. (1991). Class II antigen expressing cells in experimentally induced pulpitis. Int Endod J 24:8-14.[CrossRef][Medline]
[Order article via Infotrieve]
- Brideau RJ, Carter PB, McMaster WR, Mason DW, Williams AF (1980). Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol 10:609-615.[Medline]
[Order article via Infotrieve]
- Dallman MJ, Thomas ML, Green JR (1984). MRC 0X19: a monoclonal antibody that labels rat T lymphocytes and augments in vivo proliferative responses. Eur J Immunol 14:260-267.[Medline]
[Order article via Infotrieve]
- Dijkstra CD, Dopp EA, Joling P., Kraal G. (1985). The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 54:589-599.[Medline]
[Order article via Infotrieve]
- Hahn C.-L, Falkler WA, Siegel MA (1989). A study of T and B cells in pulpal pathosis. J Endodont 15:20-26.[Medline]
[Order article via Infotrieve]
- Hart Dnj, Fabre JW (1981). Demonstration and characterization of lapositive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain. J Exp Med 153:347-361.
- Hsu S.-M, Soban E. (1982). Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem 30:1079-1082.[Abstract]
- Ingle JI, Langeland K. (1985). Etiology and prevention of pulpal inflammation, necrosis and dystrophy. In: Ingle JI, Taintor JF, editors. Endodontics. 3rd ed. Philadelphia (PA): Lea and Febiger, 304-388.
- Jefferies WA, Green JR, Williams AF (1985). Authentic T helper CD4 (W3/ 25) antigen on rat peritoneal macrophages. J Exp Med 162:117-127.[Abstract/Free Full Text]
- Jontell M., Bergenholtz G., Scheynius A., Ambrose W. (1988). Dendritic cells and macrophages expressing class II antigens in the normal rat incisor pulp. J Dent Res 67:1263-1266.[Abstract/Free Full Text]
- Jontell M., Gunraj MN, Bergenholtz G. (1987). Immunocompetent cells in the normal dental pulp. J Dent Res 66:1149-1153.[Abstract/Free Full Text]
- Mangkornkarn C., Steiner JC, Bohman R., Lindemann RA (1991). Flow cytometric analysis of human dental pulp tissue. J Endodont 17:49-53.[CrossRef][Medline]
[Order article via Infotrieve]
- Matsuno K., Fujii H., Kotani M. (1986). Splenic marginal zone macrophages and marginal metallophils in rats and mice. Cell Tissue Res 246:263-269.[CrossRef][Medline]
[Order article via Infotrieve]
- McMaster WR, Williams AF (1979). Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol 9:426-433.[Medline]
[Order article via Infotrieve]
- Mori S., Sawai T., Teshima T., Kyogoku M. (1988). A new decalcifying technique for immunohistochemical studies of calcified tissues, especially applicable to cell surface marker demonstration. J Histochem Cytochem 36:111-114.[Abstract]
- Seltzer S., Bender IB (1984). The pulp as connective tissue. In: Seltzer S, Bender IB, editors. The dental pulp. 3rd ed. Philadelphia (PA): J.B. Lippincott, 78-104.
- Steinman RM, Van Voorhis WC, Spalding DM (1986). Dendritic Cells. In: Weir DM, editor. Handbook of experimental immunology. 4th ed. Oxford (UK): Blackwell Scientific Publications, 1149-1153.
- Verschure PJ, Van Noorden Cjf, Dijkstra CD (1989). Macrophages and dendritic cells during the early stages of antigen-induced arthritis in rats: immunohistochemical analysis of cryostat sections of the whole knee joint. Scand J Immunol 29:371-381.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 71, No. 5,
1196-1202 (1992)
DOI: 10.1177/00220345920710051201
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