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Journal of Dental Research
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Lysozyme Enhances the Inhibitory Effects of the Peroxidase System on Glucose Metabolism of Streptococcus mutans

M. Lenander-Lumikari

Department of Community and Public Health Dentistry, , University of Turku, 20520 Turku, Finland, Institute of Dentistry, University of Turku, 20520 Turku, Finland

B. Månsson-Rahemtulla

Department of Community and Public Health Dentistry, , University of Turku, 20520 Turku, Finland

F. Rahemtulla

Department of Oral Biology, University of Alabama School of Dentistry, Box 26, UAB Station, Birmingham, Alabama 35294

The combined effect of the salivary peroxidase system and lysozyme on the glucose uptake of Streptococcus mutans NCTC 10449 was investigated. The bacteria were grown to late-exponential phase, washed, re-suspended in buffer at pH 6, and incubated with (1) 50 µg/ mL lysozyme from human milk for 60 min; (2) 7-15 µmol/L hypothiocyanous acid/hypothiocyanite for 10 min; and (3) lysozyme for 60 min prior to addition of and incubation with hypothiocyanous acid/hypothiocyanite for 10 min. Glucose uptake was initiated by adding the bacterial suspensions to 10 mL of pre-warmed 50 µmol/L glucose containing 0.98 µmol/L D-(U-14C-)-glucose, and the mixture was incubated in a shaking water-bath at 37°C. Samples were withdrawn at various time intervals, rapidly filtered through 0.45-µm membranes, washed with ice-chilled buffer, and the incorporated radioactivity determined. Lysozyme stimulated S. mutans glucose uptake slightly, but significantly inhibited S. rattus glucose metabolism. A 20-30% inhibition of radiolabeled glucose incorporation was observed with hypothiocyanous acid/hypothiocyanite alone. Incubation of the bacteria with lysozyme prior to addition of hypothiocyanous acid/hypothiocyanite containing peroxidase resulted in a total inhibition of the glucose uptake. In contrast, lysozyme in combination with hypothiocyanous acid/hypothiocyanite without peroxidase gave only a 30-50% inhibition. The addition of 5 mmol/L dithiothreitol after incubation with lysozyme and hypothiocyanous acid/hypothiocyanite eliminated the inhibition of the bacterial glucose uptake. The viability of S. mutans was not affected by treatment with any of the components used. Our results indicate that physiological concentrations of lysozyme and the salivary peroxidase system components have a synergistic effect which results in a significant inhibition of glucose metabolism by S. mutans.

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Journal of Dental Research, Vol. 71, No. 3, 484-490 (1992)
DOI: 10.1177/00220345920710031201
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