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Effects of Calcium-phosphate-based Materials on Proliferation and Alkaline Phosphatase Activity of Newborn Rat Periosteal Cells in vitro

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Tarquilio

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy, Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

M. Grano

Centro di Citomorfologia Normale e Patologica, University of Chieti, Italy

S. Colucci

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Laforgia

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

F. Mangini

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Zambonin Zallone

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

The effects of dental materials, intended for bone substitution, on cell growth and alkaline phosphatase activity of newborn rat periosteal cells have been studied in vitro. Confluent periosteal cells were exposed to three apatite-based materials (400 µg/mL) with different physico-chemical properties. The materials were a β-tricalcium phosphate with a microporous granular structure obtained by sinterization (Synthograft, Johnson & Johnson, East Windsor, NY), a 40-60-mesh microporous durapatite ceramic (Periograf, Sterling Drug, Inc., Rensselaer, NY), and a 1-2-mm-diameter hydroxyapatite ceramic (Osprovit, Feldmuhle Aktiengeselschaft, Plochingen, Germany) with macropores larger than 100 µm. Cell proliferation and alkaline phosphatase activity were assessed by incorporation of 3H-thymidine into trichloroacetic-acid-precipitable material and by a fluorimetric method, respectively. Cell viability and compatibility with the materials were determined by morphology in phase-contrast microscopy. Periosteal cells showed increased proliferation following exposure to Synthograft, but were unaffected by Osprovit, whereas Periograf caused significantly reduced cell growth. Alkaline phosphatase activity was unaffected by Osprovit, but was decreased by both Synthograft and Periograf. The results indicated a differential response of periosteal cells to bone-substituting materials with heterogeneous physico-chemical characteristics.

REFERENCES

• Amler, M.H. (1987): Osteogenic Potential of Nonvital Tissues and Synthetic Implant Materials, J Periodontol 58:758-761.[Medline] [Order article via Infotrieve]
• Baron, R. (1990): Anatomy and Ultrastructure of Bone. In: Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, M.J. Favus, Ed., Richmond, VA: William Byrd Press, pp. 3-6.
• Bolander, M.E. and Bolian, G. (1986): The Use of Demineralized Bone Matrix in the Repair of Sequential Defects, J Bone Joint Surg 68:1264-1274.[Abstract/Free Full Text]
• Carano, A.; Teti, A.; and Zambonin Zallone, A. (1987): Riassorbimento di Beta-Fosfato Tricalcico ed Idrossiapatite Sintetica in vivo ed in vitro, Odontostomatologia e Impiantoprotesi 4:84-87.
• Chang, P. (1981): Polymer Implant Material with Improved X-Ray Opacity and Biocompatibility, Biomaterials 2:151-155.[CrossRef][Medline] [Order article via Infotrieve]
• Cheal, E.J.; Snyder, B.D.; Nunamaker, D.M.; and Hayes, W.C. (1987): Trabecular Bone Remodeling around Smooth and Porous Implants in an Equine Patellar Model, J Biomechanics 20:1121-1134.[CrossRef][Medline] [Order article via Infotrieve]
• Cheung, H.S. and Haak, M.H. (1989): Growth of Osteoblasts on Porous Calcium Phosphate Ceramic: an in vitro Model for Biocompatibility Study, Biomaterials 10:63-67.[CrossRef][Medline] [Order article via Infotrieve]
• Ellender, G.; Ham, K.N.; and Harcourt, J.K. (1974): Toxic Effects of Dental Amalgam Implants: Optical and Histochemical Observations, Aust Dent J 23:395-400.
• Flatley, T.J.; Lynch, K.I.; and Benson, M. (1983): Tissue Response to Implants of Calcium Phosphate Ceramic in the Rabbit, Clin Orthop Rel Res 179:246-252.[Medline] [Order article via Infotrieve]
• Gehron-Robey, P. and Termine, J.D. (1985): Human Bone Cells in vitro, Calcif Tissue Int 37:453-460.[Medline] [Order article via Infotrieve]
• Gross, U.; Kinne, R.; Schmitz, H.-J.; and Strunz, U. (1988): The Response of Bone to Surface-active Glasses/Glass-Ceramics, CRC Crit Rev Biocomp 4:155-179.
• Guenther, H.L.; Hofstetter, W.; Stutzer, A.; Mühl-Bauer, R.; and Fleisch, H. (1989): Evidence for Heterogeneity of the Osteoblastic Phenotype Determined with Clonal Rat Bone Cells Established from Transforming Growth Factor-β-induced Cell Colonies Grown Anchorage Independently in Semisolid Medium, Endocrinology 125:2092-2102.[Abstract/Free Full Text]
• Haugen, E. and Mjor, I.A. (1978): Subcutaneous Implants for Assessments of Dental Materials with Emphasis on Periodontal Dressings, J Periodont Res 13:262-269.[CrossRef][Medline] [Order article via Infotrieve]
• Heimke, G. and Griss, P. (1983): Tissue Interaction to Bone Replacement Material. In: Bioceramics of Calcium Phosphate, K. de Groot, Ed., Boca Raton, FL: CRC Press, Inc., pp. 79-94.
• Hench, L.L. and Wilson, T. (1984): Surface-active Biomaterials, Science 226:630-636.[Abstract/Free Full Text]
• Kaban, L.B. and Glowacki, J. (1981): Induced Osteogenesis in the Repair of Experimental Mandibular Defects in Rats, J Dent Res 60:136.
• Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
• Marie, P.J.; Lomri, A.; Sabbagh, A.; and Basle, N. (1989): Culture and Behaviour of Osteoblast Cells Isolated from Normal Trabecular Bone Surface, In Vtro Cell Dev Biol 25:373-380.[CrossRef]
• Menè, P. and Dunn, J.M. (1990): Prostaglandins and Rat Glomerular Mesangial Cell Proliferation, Kidney Int 37:1256-1262.[Medline] [Order article via Infotrieve]
• Mors, C. and Kaminsky, G.C. (1975): Osteogenic Replacement of Tricalcium Phosphate Ceramic Implants in the Dog Palate, Arch Oral Biol 20:365.[CrossRef][Medline] [Order article via Infotrieve]
• Moskow, B.S. and Lubarr, A. (1983): Histological Assessment of Human Periodontal Defect after Durapatite Ceramic Implant, J Periodontol 54:455-462.[Medline] [Order article via Infotrieve]
• Mulliken, J.B. and Glowacki, J. (1980): Induced Osteogenesis for Repair and Construction in the Craniofacial Region, Plast Reconstr Surg 65:553-559.[Medline] [Order article via Infotrieve]
• Nery, E.B. and Lynch, K.L. (1978): Preliminary Clinical Study of Bioceramic in Periodontal Osseous Defects, J Periodontol 49:523-527.[Medline] [Order article via Infotrieve]
• Nery, E.B.; Lynch, K.L.; and Rooney, G.E. (1978): Alveolar Ridge Augmentation with Tricalcium Phosphate, J Prosthet Dent 40:668-675.[CrossRef][Medline] [Order article via Infotrieve]
• Oikarinen, J. and Korhonen, L.K. (1979): Repair of Bone Defects by Inductive Material, Acta Orthop Scand 50:21-26.[Medline] [Order article via Infotrieve]
• Rabalais, M.L.; Yukna, R.A.; and Mayer, E.T. (1983): Evaluation of Durapatite Ceramic as an Alloplastic Implant in Periodontal Osseous Defects, J Periodontol 52:680-686.
• Rae, T. (1986): The Biological Response to Titanium Aluminum-Vanadium Alloy Particles, Biomaterials 7:37-40.[CrossRef][Medline] [Order article via Infotrieve]
• Shimazaky, K. and Moonry, V. (1985): Comparative Study of Porous Hydroxyapatite and Tricalcium Phosphate as Bone Substitute, J Orthop Res 3:301-310.[CrossRef][Medline] [Order article via Infotrieve]
• Stahl, S. and Fraum, S. (1986): Histological Evaluation of Human Intraosseous Healing Responses to the Placement of Tricalcium Phosphate Ceramic Implants, J Periodontol 57:211-217.[Medline] [Order article via Infotrieve]
• Turner, R.T.; Farley, J.; Vandersteenhoven, J.J.; Ep-Stein, S.; Bell, N.H.; and Baylink, D.J. (1988): Demonstration of Reduced Mitogenic Osteoinductive Activities in Demineralized Allogenic Bone Matrix from Vitamin-D Deficient Rats, J Clin Invest 82:212-217.[CrossRef][Medline] [Order article via Infotrieve]
• Urist, M.R. (1965): Bone Formation by Autoinduction, Science 150:893-899.[Abstract/Free Full Text]
• Yukna, R.A.; Harrison, B.G.; Caudill, R.F.; Evans, G.M.; Mayer, E.T.; and Miller, S. (1985): Evaluation of Durapatite Ceramic as an Alloplastic Implant in Periodontal Osseous Defects, J Periodontol 56:541-547.

Journal of Dental Research, Vol. 70, No. 6, 997-1001 (1991)
DOI: 10.1177/00220345910700061801


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Teti, A. Articles by Zambonin Zallone, A. Search for Related Content PubMed PubMed Citation Articles by Teti, A. Articles by Zambonin Zallone, A. Social Bookmarking                         What's this?