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Influence of Fluoride and Carbonate on in vitro Remineralization of Bovine Enamel

Biochemistry MF, Trigon

J.M.P.M. Borggreven

Biochemistry MF, Trigon

F.C.M. Driessens

Dental School, Catholic University, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

J.W.E. Van Dijk

Radiological Service TNO, P.O. Box 9034, 6800 ES, Arnhem, The Netherlands

The influence of fluoride, carbonate, and fluoride in combination with carbonate on the in vitro remineralization of bovine enamel was investigated with the use of a sandwich technique. After demineralization, enamel slices were subjected for 610 h to remineralizing solutions with 0.03 or 1.0 ppm fluoride. At each fluoride level, either 0, 1, 10, 20, or 25 mmol/L carbonate was tested. After 0, 22, 62, 126, 192, 329, and 610 h of remineralization, contact microradiographs were made by Cu K-radiation. At 0.03 ppm fluoride, carbonate had an inhibiting influence on remineralization. At 1.0 ppm fluoride, the inhibiting influence of carbonate changed into a stimulation of remineralization at 20 and 25 mmol/L carbonate. At 0, 1, and 10 mmol/L carbonate, fluoride had an inhibiting influence on remineralization. The differences in remineralization between the groups were explained by events concerning crystal growth, i.e., different types of minerals might have precipitated with differences in precipitation rates, and retardation of a precipitation step might have occurred under the various remineralization conditions. There was a mutual influence of fluoride and carbonate on the remineralization process. We conclude that the composition of the remineralizing solution with respect to fluoride and carbonate concentrations is important for the remineralization process.

REFERENCES

• Angmar, B.; Carlstrom, D.; and Glass, J.E. (1963): Studies on the Ultrastructure of Dental Enamel. IV. The Mineralization of Natural Human Enamel, J Ultrastruct Res 8:12-23.[Medline] [Order article via Infotrieve]
• Backer Dirks, O. (1966): Posteruptive Changes in Dental Enamel, J Dent Res 45:503-511.[Free Full Text]
• Brudevold, F.; Tehrani, A.; and Cruz, R. (1982): The Relationship among the Permeability to Iodide, Pore Volume, and Intra-oral Mineralization of Abraded Enamel, J Dent Res 61:645-664.[Abstract/Free Full Text]
• Driessens, F.C.M. (1982): Mineral in Tooth Enamel; Equilibration with Saliva. In: Mineral Aspects of Dentistry, H.M. Myers, Ed., Basel: Karger, pp. 49-71.
• Driessens, F.C.M.; Borggreven, J.M.P.M.; and Verbeeck, R.M.H. (1987): The Dynamics of Biomineral Systems, Bull Soc Chim Belg 96:173-179.
• Eanes, E.D. (1980): The Influence of Fluoride on the Seeded Growth of Apatite from Stable Supersaturated Solutions at pH 7.4, J Dent Res 59:144-150.[Abstract/Free Full Text]
• Eanes, E.D. and Meyer, J.L. (1978): The Influence of Fluoride on Apatite Formation from Unstable Supersaturated Solutions at pH 7.4, J Dent Res 57:617-624.[Abstract/Free Full Text]
• Feagin, F.F.; Thiradilok, S.; Aponte-Merced, L.; and Bradley, E. (1977): Effects of Bicarbonate on Remineralization of Enamel, J Oral Pathol 6:331-337.[CrossRef][Medline] [Order article via Infotrieve]
• FEAGIN, F.F.; WALKER, A.A.; and PIGMAN, W. (1969): Evaluation of the Calcifying Characteristics of Biological Fluids and Inhibitors of Calcification, Calcif Tissue Res 4:231-244. GROENEVELD, A. (1974): Dental Caries. Some Aspects of Artificial Caries Lesions Examined by Contact-Microradiography, Thesis, University of Utrecht, The Netherlands.
• Ingram, G.S. (1973): The Role of Carbonate in Dental Enamel, Caries Res 7:217-230.[Medline] [Order article via Infotrieve]
• JENKINS, G.N. (1978): The Physiology and Biochemistry of the Mouth, 4th ed., Oxford: Blackwell Scientific Publications. KOULOURIDES, T. and CAMERON, B. (1980): Enamel Mineralisation as a Factor in the Pathogenesis of Dental Caries, J Oral Pathol 9:255-269.
• Koulourides, T.; Feagin, F.F.; and Pigman, W. (1965): Remineralization of Dental Enamel by Saliva in vitro, Ann NY Acad Sci 131:751.[CrossRef][Medline] [Order article via Infotrieve]
• Lammers, P.C.; Borggreven, J.M.P.M.; and Driessens, F.C.M. (1990a): Influence of Fluoride on in vitro Remineralization of Artificial Subsurface Lesions Determined with a Sandwich Technique, Caries Res 24:81-85.[Medline] [Order article via Infotrieve]
• Lammers, P.C.; Borggreven, J.M.P.M.; and Driessens, F.C.M. (1990b): The Influence of Fluoride on in vitro Remineralization of Bovine Enamel, Bull Group Int Rech Sci Stomatol Odontol 33:199-203.
• Larsen, M.J. and Fejerskov, O. (1989): Chemical and Structural Challenges in Remineralization of Dental Enamel Lesions, Scand J Dent Res 97:285-296.[Medline] [Order article via Infotrieve]
• McCANN, H.G. (1968): Inorganic Components of Salivary Secretions. In: Art and Science of Dental Caries Research, R.S. Harris, Ed., New York: Academic Press, pp. 55-73. McCONNELL, D. (1973): Apatite. Its Crystal Chemistry, Mineralogy, Utilization, and Geologic and Biologic Occurrences, A.V.D. Frechette, E.H. Kirsch, W.L.B. Sand, and L.F. Trojer, Eds., Vienna: Springer Verlag.
• Meyer, J.L. and Nancollas, G.E. (1972): Effect of Stannous and Fluoride Ions on the Rate of Crystal Growth of Hydroxyapatite, J Dent Res 51:1443-1450.[Abstract/Free Full Text]
• NAESSENS, D.; VERBEECK, R.M.H.; and DRIESSENS, F.C.M. (1989): Carbonated Substitution Mechanisms in Precipitated Highly-carbonated Hydroxyapatites, J Dent Res 68:1003, Abst. No. 1089. OKAZAKI, M.; TAKAHASHI, J.; KIMURA, H.; and AOBA, T. (1982): Crystallinity, Solubility, and Dissolution Rate Behavior of Fluoridated C03 Apatites, J Biomed Mater Res 16:851-860. POT, T.J. and GROENEVELD, A. (1976): Het Ontstaan en Gedrag van de Witte Vlek; Overwegingen aan de Hand van Klinische Waamemingen, Ned Tijdschr T 83:464-471.
• ROBINSON, R.A. and STOKES, R.H. (1970): Electrolyte Solutions, 2nd ed. (revised), London: Butterworths. SILVERSTONE, L.M. and WEFEL, J.S. (1981): The Effect of Remineralization on Artificial Caries-like Lesions and their Crystal Content, J Cryst Growth 53:148-159.
• Silverstone, L.M.; Wefel, J.S.; Zimmerman, B.E.; Clarkson, B.H.; and Featherstone, M.J. (1981): Remineralization of Natural and Artificial Lesions in Human Dental Enamel in vitro. Effect of Calcium Concentration of the Calcifying Fluid, Caries Res 15:138-157.[Medline] [Order article via Infotrieve]
• Ten Cate, J.M. and Arends, J. (1977): Remineralization of Artificial Enamel Lesions in vitro, Caries Res 11:277-286.[Medline] [Order article via Infotrieve]
• Ten Cate, J.M. and Arends, J. (1980): Remineralization of Artificial Enamel Lesions in vitro. III. A Study of the Deposition Mechanism, Caries Res 14:351-358.[Medline] [Order article via Infotrieve]
• Ten Cate, J.M. and Duusters, P.P.E. (1982): Alternating Demineralization and Remineralization of Artificial Enamel Lesions, Caries Res 16:201-210.[Medline] [Order article via Infotrieve]
• Ten Cate, J.M.; Timmer, K.; Shariati, M.; and Featherstone, J.D.B. (1988): Effect of Timing of Fluoride Treatment on Enamel De- and Remineralization in vitro: a pH-cycling Study, Caries Res 22:20-26.[Medline] [Order article via Infotrieve]
• Theuns, H.M.; Van Duk, J.W.E.; Driessens, F.C.M.; and Groeneveld, A. (1984): The Effect of Undissociated Acetic-Acid Concentration of Buffer Solutions on Artificial Caries-like Lesion Formation in Human Tooth Enamel, Arch Oral Biol 29:759-763.[CrossRef][Medline] [Order article via Infotrieve]
• Theuns, H.M.; Van Duk, J.W.E.; Driessens, F.C.M.; and Groeneveld, A. (1985): Effect of Time, Degree of Saturation, pH and Acid Concentration of Buffer Solutions on the Rate of in-vitro Demineralization of Human Enamel, Arch Oral Biol 30:37-42.[CrossRef][Medline] [Order article via Infotrieve]
• Van Dijk, J.W.E.; Borggreven, J.M.P.M.; and Driessens, F.C.M. (1979): Chemical and Mathematical Simulation of Caries, Caries Res 13:169-180.[Medline] [Order article via Infotrieve]
• Vignoles, C.; Bonel, G.; and Montel, G. (1975): Sur la Composition des Apatites Carbonates de Type B Preparées en Milieu Sode, C r hebd Seanc Acad Sci Paris Ser C 280:275-277.

Journal of Dental Research, Vol. 70, No. 6, 970-974 (1991)
DOI: 10.1177/00220345910700061201


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