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Human Dentin-matrix-derived Bone Morphogenetic Protein
K. Bessho
Department of Oral and Maxillofacial Surgery, Mie University, School of Medicine, 2-174 Edobashi, Tsu, Mie 514, Japan
N. Tanaka
Department of Oral and Maxillofacial Surgery, Mie University, School of Medicine, 2-174 Edobashi, Tsu, Mie 514, Japan
J. Matsumoto
Department of Oral and Maxillofacial Surgery, Mie University, School of Medicine, 2-174 Edobashi, Tsu, Mie 514, Japan
T. Tagawa
Department of Oral and Maxillofacial Surgery, Mie University, School of Medicine, 2-174 Edobashi, Tsu, Mie 514, Japan
M. Murata
Department of Oral and Maxillofacial Surgery, Mie University, School of Medicine, 2-174 Edobashi, Tsu, Mie 514, Japan
Bone morphogenetic protein (BMP) was extracted from human dentin matrix with 4 mol/L guanidine-HCl and was purified by liquid chromatography. SDS-PAGE and IEF showed that the purified BMP was homogeneous and induced new bone formation in situ after three weeks when implanted into muscle pouches in Wistar rats. The molecular weight of BMP was estimated to be about 20.0 kDa by SDS-PAGE, and the pI value was 8.8 by IEF. Amino acid analysis suggested that BMP is a protein containing 191 amino acids. A partial amino acid sequence was obtained from the final purified BMP. Dentin-matrix-derived BMP is probably not identical to, but is similar to, bone-matrix-derived BMP, though both types of BMP have the same action in vivo.
REFERENCES
- Bauer, F.C.H. and Urist, M.R. (1981): Human Osteosarcoma-derived Soluble Bone Morphogenetic Protein, Clin Orthop 154:291-295.[Medline]
[Order article via Infotrieve]
- Bentz, H.; Nathan, R.M.; Rosen, D.M.; Armstrong, R.M.; Thompson, A.Y.; Segarini, P.R.; Mathews, M.C.; Dasch, J.R.; Piez, K.A.; and Seyedin, S.M. (1989): Purification and Characterization of a Unique Osteoinductive Factor from Bovine Bone, J Biol Chem 264:20805-20810.[Abstract/Free Full Text]
- Bessho, K.; Tagawa, T.; and Murata, M. (1989): Purification of Bone Morphogenetic Protein Derived from Bovine Bone Matrix, Biochem Biophys Res Commun 165:595-601.[CrossRef][Medline]
[Order article via Infotrieve]
- Bessho, K.; Tagawa, T.; and Murata, M. (1990): Purification of Rabbit Bone Morphogenetic Protein Derived from Bone, Dentin and Wound Tissue after Tooth Extraction, J Oral Maxillofac Surg 48:162-169.[Medline]
[Order article via Infotrieve]
- Butler, W.T.; Mikulski, A.; and Urist, M.R. (1977): Non-collagenous Proteins of a Rat Dentin Matrix Possessing Bone Morphogenetic Activity, J Dent Res 56:228-232.[Abstract/Free Full Text]
- Conover, M.A. and Urist, M.R. (1979): Transmembrane Bone Morphogenesis by Implants of Dentin Matrix, J Dent Res 58:1911.[Free Full Text]
- Hanamura, H.; Higuchi, Y.; Nakagawa, M.; Iwata, H.; Nogami, H.; and Urist, M.R. (1980): Solubilized Bone Morphogenetic Protein (BMP) from Mouse Osteosarcoma and Rat Demineralized Bone Matrix, Clin Orthop 148:281-290.[Medline]
[Order article via Infotrieve]
- Kawai, T. and Urist, M.R. (1989): Bovine Tooth-derived Bone Morphogenetic Protein, J Dent Res 68:1069-1074.[Abstract/Free Full Text]
- Luyten, F.P.; Cunningham, N.S.; Ma, S.; Muthukumaran, N.; Hammonds, R.G.; Nevins, W.B.; Wood, W.I.; and Reddi, A.H. (1989): Purification and Partial Amino Acid Sequence of Osteogenin, a Protein Initiating Bone Differentiation, J Biol Chem 264:13377-13380.[Abstract/Free Full Text]
- Muthukumaran, N.; Sampath, T.K.; and Reddi, A.H. (1985): Comparison of Bone Inductive Proteins of Rat and Porcine Bone Matrix, Biochem Biophys Res Commun 131:37-41.[Medline]
[Order article via Infotrieve]
- Nilsson, O.S.; Urist, M.R.; Dawson, E.G.; Schmalzried, T.P.; and Finerman, G.A.M. (1986): Bone Repair Induced by Bone Morphogenetic Protein in Ulnar Defects in Dogs, J Bone Joint Surg (Br) 68-B:635-642.
- Plank, J. and Rychlo, A. (1952): Eine Schnellentkalkungsmethode, Zentralb Allg Pathol 89:252-254.
- Sampath, T.K.; Muthukumaran, N.; and Reddi, A.H. (1987): Isolation of Osteogenin, an Extracellular Matrix-associated Bone-inductive Protein, by Heparin Affinity Chromatography, Proc Natl Acad Sci USA 84:7109-7113.[Abstract/Free Full Text]
- Sampath, T.K.; Nathanson, M.A.; and Reddi, A.H. (1984): In vitro Transformation of Mesenchymal Cells Derived from Embryonic Muscle into Cartilage in Response to Extracellular Matrix Components of Bone, Proc Natl Acad Sci USA 81:3419-3423.[Abstract/Free Full Text]
- Sampath, T.K. and Reddi, A.H. (1983): Homology of Bone-inductive Proteins from Human, Monkey, Bovine and Rat Extracellular Matrix, Proc Natl Acad Sci USA 80:6591-6595.[Abstract/Free Full Text]
- Sato, K.; Miura, T.; and Iwata, H. (1988): Cartilaginous Transdifferentiation of Rat Tenosynovial Cells under the Influence of Bone Morphogenetic Protein in Tissue Culture, Clin Orthop 236:233-239.[Medline]
[Order article via Infotrieve]
- Sato, K. and Urist, M.R. (1985): Induced Regeneration of Calvaria by Bone Morphogenetic Protein (BMP) in Dogs, Clin Orthop 197:301-311.[Medline]
[Order article via Infotrieve]
- Seyedin, S.M.; Thomas, T.C.; Thompson, A.Y.; Rosen, D.M.; and Piez, K.A. (1985): Purification and Characterization of Two Cartilage-inducing Factors from Bovine Demineralized Bone, Proc Natl Acad Sci USA 82:2267-2271.[Abstract/Free Full Text]
- Shimomura, Y.; Yoneda, T.; and Suzuki, F. (1975): Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib, Calcif Tissue Res 19:179-187.[Medline]
[Order article via Infotrieve]
- Takaoka, K.; Ono, K.; Amitani, K.; Kishimoto, R.; and Nakata, Y. (1980): Solubilization and Concentration of a Bone-inducing Substance from a Murine Osteosarcoma, Clin Orthop 148:274-280.[Medline]
[Order article via Infotrieve]
- Urist, M.R. (1965): Bone: Formation by Autoinduction, Science 150:893-899.[Abstract/Free Full Text]
- Urist, M.R.; Chang, J.J.; Lietze, A.; Huo, Y.K.; Brownell, A.G.; and Delange, R.J. (1987): Preparation and Bioassay of Bone Morphogenetic Protein and Polypeptide Fragments, Methods Enzymol 146:294-312.[Medline]
[Order article via Infotrieve]
- Urist, M.R.; Lietze, A.; Mizutani, H.; Takagi, K.; Trif-Fitt, J.T.; Amstutz, J.; Delange, R.; Termine, J.; and Finerman, G.A.M. (1982): A Bovine Low Molecular Weight Bone Morphogenetic Protein (BMP) Fraction, Clin Orthop 162:219-232.[Medline]
[Order article via Infotrieve]
- Urist, M.R.; Sato, K.; Brownell, A.G.; Malinin, T.I.; Lietze, A.; Huo, Y.-K.; Prolo, D.J.; Oklund, S.; Finerman, G.A.M.; and Delange, R.J. (1983): Human Bone Morphogenetic Protein (hBMP), Proc Soc Exp Biol Med 173:194-199.[CrossRef][Medline]
[Order article via Infotrieve]
- Wang, E.A.; Rosen, V.; Cordes, P.; Hewick, R.M.; Kriz, M.J.; Luxenberg, D.P.; Sibley, B.S.; and Wozney, J.M. (1988): Purification and Characterization of Other Distinct Bone-inducing Factors, Proc Natl Acad Sci USA 85:9484-9488.[Abstract/Free Full Text]
- Wozney, J.M.; Rosen, V.; Celeste, A.J.; Mitsock, L.M.; Whitters, M.J.; Kriz, R.W.; Hewick, R.M.; and Wang, E.A. (1988): Novel Regulators of Bone Formation: Molecular Clones and Activities, Science 242:1528-1534.[Abstract/Free Full Text]
- Wright, G.C.; Miller, F.; and Sokoloff, L. (1985): Induction of Bone by Xenografts of Rabbit Growth Plate Chondrocytes in the Nude Mouse, Calcif Tissue Int 37:250-256.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 70, No. 3,
171-175 (1991)
DOI: 10.1177/00220345910700030301
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