This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Giniger, M.S. Articles by Bronner, F. Search for Related Content PubMed PubMed Citation Articles by Giniger, M.S. Articles by Bronner, F. Social Bookmarking                         What's this?

A Human Periodontal Ligament Fibroblast Clone Releases a Bone Resorption Inhibition Factor in vitro

Departments of BioStructure and Function, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

L. Norton

Department of Orthodontics, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

S. Sousa

Department of Medicine, The Veterans Administration Medical Center, Newington, Connecticut 06111

J.A. Lorenzo

Department of Medicine, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030, Department of Medicine, The Veterans Administration Medical Center, Newington, Connecticut 06111

F. Bronner

Departments of BioStructure and Function, , The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

The conditioned media (CM) obtained from three lines of cloned human periodontal ligament (PDL) cells were analyzed to determine whether they altered the parathyroid hormone (PTH)-stimulated resorption rates (45Ca release) in 48-hour cultures of ⁴⁵Ca-labeled rat long bones. One PDL cell line, PDL-5, produced a heat-resistant factor in its CM that inhibited the PTH-stimulated resorption by 43.8 ± 9.7 (SE) percent (p < 0.02), whereas the CM from the other cell lines were without statistically significant effect. The CM from the PDL-5 line did not diminish organ culture viability, as determined by 3H-thymidine incorporation, and did not enhance or diminish the resorption-inhibiting activity of calcitonin added to the PTH-stimulated cultures. The addition of CM from PDL-5 did not alter the bone-resorbing effect of interleukin-1 (IL-1). These results indicate that CM from PDL-5 inhibits only the PTHinduced and not the IL-1-mediated resorption processes, whose mechanisms are therefore likely to differ.

REFERENCES

• Anderson, D.J.; Hannam, A.G.; and Matthews, B. (1970): Sensory Mechanisms in Mammalian Teeth and Their Supporting Structures, Physiol Rev 50:171-195.[Free Full Text]
• Chambers, T.J. (1988): The Regulation of Osteoclastic Development and Function, Ciba Found Symp 136:92-107.[Medline] [Order article via Infotrieve]
• Chambers, T.J.; McSheehy, P.M.J.; Thompson, B.M.; and Fuller, K. (1985): The Effect of Calcium-regulating Hormones and Prostaglandins on Bone Resorption Osteoclasts Disaggregated from Neonatal Rabbit Bones, Endocrinol 60:234-239.
• Gowen, M.; Nedwin, G.E.; and Mundy, G.R. (1986): Preferential Inhibition of Cytokine Stimulated Bone Resorption by Recombinant Interferon Gamma, J Bone Miner Res 1:469-473.[Medline] [Order article via Infotrieve]
• Hoffmann, O.; Klaushofer, K.; Gleispach, H.; Leis, H.J.; Luger, T.; Koller, K.; and Peterlik, M. (1987): Gamma Interferon Inhibits Basal and Interleukin 1-induced Prostaglandin Production and Bone Resorption in Neonatal Mouse Calvaria, Biochem Biophys Res Commun 143:38-43.[Medline] [Order article via Infotrieve]
• Hou, L.T. (1987): Epithelial Cell-Fibroblast Interactions of Periodontal Tissues in vitro, Ph.D. Thesis, University of Connecticut, pp. 137-138.
• Jilka, R.L. (1986): Are Osteoblastic Cells Required for the Control of Osteoclast Activity by Parathyroid Hormone?, Bone Miner 1:261-266.[Medline] [Order article via Infotrieve]
• Lindskog, S. and Hammarstrom, L. (1980): Evidence In Favor of an Anti-Invasion Factor in Cementum or Periodontal Membrane of Human Teeth, Scand J Dent Res 88:161-163.[Medline] [Order article via Infotrieve]
• Lorenzo, J.A.; Quinton, J.; Sousa, S.; and Raisz, L.G. (1986): Effects of DNA and Prostaglandin Synthesis Inhibitors on the Stimulation of Bone Resorption by Epidermal Growth Factor in Fetal Rat Long-Bone Cultures, J Clin Invest 77:1897-1902.[Medline] [Order article via Infotrieve]
• Lorenzo, J.A.; Raisz, L.G.; and Hock, J.M. (1983): DNA Synthesis Is Not Necessary for Osteoclastic Responses to Parathyroid Hormone in Cultures of Fetal Rat Long Bones, J Clin Invest 72:1924-1929.[CrossRef][Medline] [Order article via Infotrieve]
• Melcher, A.H. (1970): Repair of Wounds in the Periodontium of the Rat, Arch Oral Biol 15:1183-1195.[CrossRef][Medline] [Order article via Infotrieve]
• Melcher, A.H. (1984): Cellular Activity in Adaptation of the Periodontium. In: Malocclusion and the Periodontium, J.A. McNamara, Jr., and K.A. Ribbens, Eds., Craniofacial Growth Series Monograph No. 15, Ann Arbor, Michigan: University of Michigan Press, pp. 1-15.
• Mundy, G.R. and Roodman, G.D. (1987): Osteoclast Ontogeny and Function. In: Bone and Mineral Research, Vol. 5, W.A. Peck, Ed., Amsterdam: Elsevier Science Publishers B.V., pp. 209-279.
• Pfeilschifter, J.; Seyedin, S.M.; and Mundy, G.R. (1988): Transforming Growth Factor β Inhibits Bone Resorption in Fetal Rat Long Bone Cultures, J Clin Invest 82:680-685.[Medline] [Order article via Infotrieve]
• Picton, D.C.A. (1969): The Effects of External Forces in the Periodontium. In: Biology of the Periodontium, A.H. Melcher, Ed., New York: Academic Press, pp. 363-415.
• Raisz, L.G. and Niemann, I. (1967): Effects of Parathyroid Hormone and Thyrocalcitonin on Bone Organ Culture, Nature 214:486-487.[CrossRef][Medline] [Order article via Infotrieve]
• Raisz, L.G.; Trummel, C.L.; Wener, J.A.; and Simmons, H. (1972): Effect of Glucocorticoids on Bone Resorption in Tissue Culture, Endocrinol 90:961-967.[Abstract/Free Full Text]
• Rodan, G.A. and Martin, T.J. (1981): Role of Osteoblasts in the Control of Bone Resorption-A Hypothesis, Calcif Tissue Int 33:349-351.[CrossRef][Medline] [Order article via Infotrieve]
• Zaidi, M.; Chambers, T.J.; Das, R.G.; Morris, H.R.; and McIntyre, I. (1987): A Direct Action of Human Calcitonin Gene-related Peptide on Isolated Osteoclasts, J Endocrinol 115:511-518.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 70, No. 2, 99-101 (1991)
DOI: 10.1177/00220345910700021901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				Y.-M. Wu, D.W. Richards, and D.J. Rowe Production of Matrix-degrading Enzymes and Inhibition of Osteoclast-like Cell Differentiation by Fibroblast-like Cells from the Periodontal Ligament of Human Primary Teeth Journal of Dental Research, February 1, 1999; 78(2): 681 - 689. [Abstract] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Giniger, M.S. Articles by Bronner, F. Search for Related Content PubMed PubMed Citation Articles by Giniger, M.S. Articles by Bronner, F. Social Bookmarking                         What's this?