Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Miller, C.S.
Right arrow Articles by Smith, K.O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, C.S.
Right arrow Articles by Smith, K.O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Enhanced Replication of Herpes Simplex Virus Type 1 in Human Cells

C.S. Miller

Department of Oral Health Science, Division of Oral Diagnosis, MN228 Chandler Medical Center, University of Kentucky College of Dentistry, Lexington, Kentucky 40536

K.O. Smith

Department of Microbiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78284

The effects of DNA-damaging agents on the replication of herpes simplex virus type 1 (HSV-1) were assessed in vitro. Monolayers of human lung fibroblast cell lines were exposed to DNA-damaging agents (methyl methanesulfonate [MMS], methyl methanethiosulfonate [MMTS], ultraviolet light [UV], or gamma radiation [GR]) at specific intervals, before or after inoculation with low levels of HSV-1. The ability of cell monolayers to support HSV-1 replication was measured by direct plaque assay and was compared with that of untreated control samples. In this system, monolayers of different cell lines infected with identical HSV-1 strains demonstrated dissimilar levels of recovery of the infectious virus. Exposure of DNA-repair-competent cell cultures to DNA-damaging agents produced time-dependent enhanced virus replication. Treatment with agent before virus inoculation significantly (p < 0.025) increased the number of plaques by 10 to 68%, compared with untreated control cultures, while treatment with agent after virus adsorption significantly increased (p<0.025) the number of plaques by 7 to 15%. In a parallel series of experiments, cells deficient in DNA repair (xeroderma pigmentosum) failed to support enhanced virus replication. These results suggest that after exposure to DNA-damaging agents, fibroblasts competent in DNA repair amplify the replication of HSV-1, and that DNA-repair mechanisms that act on a variety of chromosomal lesions may be involved in the repair and biological activation of HSV-1 eenomes.

REFERENCES

  • Abrahams, P.J.; Huitema, B.A.; and Van Der Eb, A.J. (1984): Enhanced Reactivation and Enhanced Mutagenesis of Herpes Simplex Virus in Normal Human and Xeroderma Pigmentosum Cells, Molec Cell Biol 4:2341-2346.[Abstract/Free Full Text]
  • Baringer, J.R. and Swoveland, P. (1973): Recovery of Herpes Simplex Virus from Human Trigeminal Ganglions, N Engl J Med 288:648-650.[Medline] [Order article via Infotrieve]
  • Ben-Porat, T.; Rixon, F.J.; and Blankenship, M.L. (1979): Analysis of the Structure of the Genome of Pseudorabies Virus, Virology 95:285-294.[CrossRef][Medline] [Order article via Infotrieve]
  • Blyth, W.A.; Hill, T.J.; Field, H.J.; and Harbour, D.A. (1976): Reactivation of Herpes Simplex Virus Infection by Ultraviolet Light and Possible Involvement of Prostaglandins, J Gen Virol 33:547-550.[Abstract/Free Full Text]
  • Bockstahler, L.E. and Lytle, C.D. (1970): Ultraviolet Light Enhanced Reactivation of a Mammalian Virus, Biochim Biophys Acta 41:184-189.
  • Fogel, B.; Yamanishi, K.; and Rapp, F. (1979): Enhancement of Host Cell Reactivation of Ultraviolet-irradiated Herpes Simplex Virus by Caffeine, Hydroxyurea and 5-bromodeoxyuridine, Int J Cancer 23:657-662.[CrossRef][Medline] [Order article via Infotrieve]
  • Gordin, M.; Olshevsky, U.; Rosenkranz, H.S.; and Becker, Y. (1973): Studies on Herpes Simplex Virus DNA: Denaturation Properties, Virology 55:280-284.[Medline] [Order article via Infotrieve]
  • Hanawalt, P.C.; Cooper, R.; Ganesan, A.K.; and Smith, C.A. (1979): DNA Repair in Bacteria and Mammalian Cells, Ann Rev Biochem 48:783-836.[CrossRef][Medline] [Order article via Infotrieve]
  • Hill, T.J. (1985): Herpes Simplex Virus Latency. In: The Herpes-viruses, Volume 3, B. Roizman, Ed., New York: Plenum Press, pp. 175-240.
  • Hirsch, I. and Vonka, V. (1974): Ribonucleotides Linked to DNA of Herpes Simplex Virus Type 1, J Virol 13:1162-1168.[Abstract/Free Full Text]
  • Hyman, R.W.; Oakes, J.E.; and Kudler, L. (1977): In vitro Repair of the Pre-existing Nicks and Gaps in Herpes Simplex Virus DNA, Virology 76:286-294.[CrossRef][Medline] [Order article via Infotrieve]
  • Kieff, E.D.; Bachenheimer, S.L.; and Roizman, B. (1971): Size, Composition, and Structure of Deoxyribonucleic Acid of Herpes Simplex Virus Subtypes 1 and 2, J Virol 8:125-132.[Abstract/Free Full Text]
  • Kolber, A.R. (1975): In vitro Synthesis of DNA in Nuclei Isolated from Human Lung Cells Infected with Herpes Simplex Type II Virus, J Virol 15:322-331.[Abstract/Free Full Text]
  • Lorentz, A.K.; Munk, K.; and Darai, G. (1977): DNA Repair Replication in Human Embryonic Lung Cells Infected with Herpes Simplex Virus, Virology 82:401-408.[CrossRef][Medline] [Order article via Infotrieve]
  • Lytle, C.D. (1971): Host-cell Reactivation in Mammalian Cells. I. Survival of Ultra-violet-irradiated Herpes Virus in Different Cell-lines, Int J Radiat Biol 19:329-337.[CrossRef]
  • Nishiyama, Y. and Rapp, F. (1981): Repair Replication of Viral and Cellular DNA in Herpes Simplex Virus Type 2-Infected Human Embryonic and Xeroderma Pigmentosum Cells, Virology 110:466-475.[CrossRef][Medline] [Order article via Infotrieve]
  • Openshaw, H.; Asher, L.V.S.; Wohlenberg, C.; Sekizawa, T.; and Notkins, A.L. (1979): Acute and Latent Herpes Simplex Virus Ganglionic Infection: Immune Control and Viral Reactivation, J Gen Virol 44:205-215.[Abstract/Free Full Text]
  • Perna, J.J.; Mannix, M.L.; Rooney, J.F.; Notkins, A.L.; and Straus, S.E. (1987): Reactivation of Latent Herpes Simplex Infection by Ultraviolet Light: A Human Model, J Am Acad Dermatol 17:473-478.[Medline] [Order article via Infotrieve]
  • Price, R.W. (1979): 6-Hydroxydopamine Potentiates Acute Herpes Simplex Virus Infection of the Superior Cervical Ganglion in Mice, Science 205:518-520.[Abstract/Free Full Text]
  • Puga, A.; Cantin, E.M.; Wohlenberg, C.; Openshaw, H.; and Notkins, A.L. (1984): Different Sizes of Restriction Endonuclease Fragments from the Terminal Repetitions of the Herpes Simplex Virus Type 1 Genome Latent in Trigeminal Ganglia of Mice, J Gen Virol 65:437-444.[Abstract/Free Full Text]
  • Reed, L.J. and Muench, H. (1938): A Simple Method of Estimating Fifty Per Cent Endpoints, Am J Hyg 27:493-496.
  • Rock, D.L. and Fraser, N.W. (1983): Detection of HSV-1 Genome in Central Nervous System of Latently Infected Mice, Nature 302:523-525.[CrossRef][Medline] [Order article via Infotrieve]
  • Roizman, B. and Roane, P.R. (1961): A Physical Difference Between Two Strains of Herpes Simplex Virus Apparent on Sedimentation in Cesium Chloride, Virology 15:75-79.[CrossRef][Medline] [Order article via Infotrieve]
  • Sanes, J.R. and Okun, L.M. (1972): Induction of DNA Synthesis in Cultured Neurons by Ultraviolet Light or Methyl Methane Sulfonate, J Cell Biol 53:587-594.[Free Full Text]
  • Shimizu, F.; Monma, Y.; Sekizawa, T.; and Kamiyawa, J.K. (1989): Establishment of Latent Ganglionic Infection with Herpes Simplex Virus via Maxillary Gingiva and Viral Re-activation in vivo after Trauma, J Dent Res 68:472-475.[Abstract/Free Full Text]
  • Spruance, S.L. (1988): Cutaneous Herpes Simplex Virus Lesions Induced by Ultraviolet Radiation, Am J Med 85:43-45.[Medline] [Order article via Infotrieve]
  • Valerie, K.; Delers, A.; Bruck, C.; Thiriart, C.; Rosenberg, H.; Debouck, C.; and Rosenberg, M. (1988): Activation of Human Immunodeficiency Virus Type 1 by DNA Damage in Human Cells, Nature 333:78-81.[CrossRef][Medline] [Order article via Infotrieve]
  • Walz, M.A.; Price, R.W.; and Notkins, A.L. (1974): Latent Ganglionic Infection with Herpes Simplex Virus Types 1 and 2: Viral Reactivation in vivo after Neurectomy, Science 184:1185-1187.[Abstract/Free Full Text]
  • Warren, K.G.; Brown, S.M.; Wroblewska, Z.; Cil-Dern, D.; Koprowski, H.; and Subak-Sharpe, J. (1978): Isolation of Herpes Simplex Virus from the Superior Cervical and Vagus Ganglions of Human Beings, N Engl J Med 298:1068-1069.[Medline] [Order article via Infotrieve]
  • Wilkie, N.M. (1973): The Synthesis and Substructure of Herpes-virus DNA: The Distribution of Alkali-labile Single Strand Interruptions in HSV-1 DNA, J Gen Virol 21:453-467.[Abstract/Free Full Text]
  • Wu, C.; Harper, L.; and Ben-Porat, T. (1986): Molecular Basis for Interference of Defective Interfering Particles of Pseudorabies Virus with Replication of Standard Virus, J Virol 59:308-317.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 70, No. 2, 111-117 (1991)
DOI: 10.1177/00220345910700020301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
C.S. Miller, R.J. Danaher, and R.J. Jacob
Molecular Aspects of Herpes Simplex Virus I Latency, Reactivation, and Recurrence
Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(4): 541 - 562.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Miller, C.S.
Right arrow Articles by Smith, K.O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, C.S.
Right arrow Articles by Smith, K.O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?