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In vitro Demineralization of Enamel by F-sensitive and F-resistant Mutans Streptococci in the Presence of 0, 0.05, or 0.5 mmol/L NaF
C. Van Loveren
Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands
L.M. Spitz
Department of Oral Biology Eastman Dental Center, 625 Elmwood Avenue Rochester, New York 14620
J.F. Buijs
Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands
J.M. Ten Cate
Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands
A.D. Eisenberg
Department of Oral Biology Eastman Dental Center, 625 Elmwood Avenue Rochester, New York 14620
Lactate production and accompanying enamel demineralization by fluoride-sensitive and fluoride-resistant mutans streptococci were studied in an in vitro demineralization model in the presence of 0, 0.05, or 0.5 mmol/L NaF. The fluoride-resistant strains were derived from laboratory strains or were recently isolated strains from xerostomic patients on high-dose fluoride therapy. The demineralization model was composed of a cell suspension in a glucose-agarose gel overlying a bovine enamel block. Lactate and calcium content of the agarose were determined after 22-hour incubations at 37°C.
Fluoride-resistant variants of Streptococcus sobrinus 6715-15 produced less lactate and caused less demineralization than did the parent strain even in the presence of fluoride. On the other hand, fluoride-resistant variants of Streptococcus mutans C180-2 and of S. mutans GS-5 produced more acid and caused greater demineralization than did their respective parent strains, both in the absence and presence of fluoride. Two recently isolated fluoride-resistant S. mutans strains produced more lactate and demineralized enamel more than did two recently isolated S. mutans strains from normal human subjects, both in the presence of 0 and 0.05 mmol/L NaF.
It is concluded that adaptation to fluoride resistance does not invariably reduce the cariogenicity of mutans streptococci nor the effectiveness of fluoride in preventing demineralization.
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Journal of Dental Research, Vol. 70, No. 12,
1491-1496 (1991)
DOI: 10.1177/00220345910700120401
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