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Presence of Vesicles Containing Lactate Dehydrogenase in the Dentin of Bovine Tooth Germs

Department of Operative Dentistry, Kyushu Dental College, Manazuru 2-6-1, Kokurakita-ku, Kitakyushu 803, Japan

M. Terashita

Department of Operative Dentistry, Kyushu Dental College, Manazuru 2-6-1, Kokurakita-ku, Kitakyushu 803, Japan

T. Noguchi

Department of Biochemistry, Kyushu Dental College, Manazuru 2-6-1, Kokurakita-ku, Kitakyushu 803, Japan

The dentin was removed from bovine tooth germs, followed by the separation of the extracellular matrix vesicle fraction after collagenase treatment. Lactate dehydrogenase (LDH)-containing vesicles with a density different from that of matrix vesicles were detected in the matrix vesicle fraction. LDH in these vesicles did not result from cell lysis and vesicle capture during the preparation of the matrix vesicle fraction. The isoenzyme pattern of LDH in LDHcontaining vesicles was similar to that of cytosolic LDH of odontoblasts. Other cytosolic enzymes were not detected in LDH-containing vesicles, suggesting the presence of a mechanism for specific uptake of cytosolic LDH during the in vivo formation of the vesicles.

REFERENCES

• Ali, S.Y.; Sajdera, S.W.; and Anderson, H.C. (1970): Isolation and of Calcifying Matrix Vesicles from Epiphyseal Cartilage, Proc Natl Acad Sci USA 67:1513-1520.[Abstract/Free Full Text]
• Anderson, H.C. (1969): Vesicles Associated with Calcification in the Matrix of Epiphyseal Cartilage, J Cell Biot 41:59-72.[CrossRef]
• Bergmeyer, H.U. and Bernt, E. (1974a): Lactate Dehydrogenase. UV-Assay with Pyruvate and NADH. In: Methods of Enzymatic Analy. sis, Vol. 2, H.U. Bergmeyer, Ed., New York: Academic Press, pp. 574-579.
• Bergmeyer, H.U. and Bernt, E. (1974b): Glutamate-Oxaloacetate Transaminase. UV-Assay, Manual Method. In: Methods of Enzymatic Analysis, Vol. 2, H.U. Bergmeyer, Ed., New York: Academic Press, pp. 727-733.
• Bergmeyer, H.U. and Bernt, E. (1974c): Glutamate-PyruvateTransaminase. OV-Assay, Manual Method, In: Methods of Enzymatic Analysis, Vol. 2, H.U. Bergmeyer, Ed., New York: Academic Press, pp. 752-758.
• Bergmeyer, H.U.; Gawehn, K.; and Grassl, M. (1974): Enzymes as Biochemical Reagents. Aldolase. In: Methods of Enzymatic Analysis, Vol. 1, H.U. Bergmeyer, Ed., New York: Academic Press, p. 430.
• Bernard, G.W. and Pease, D.C. (1969): An Electron Microscopic Study of Initial Intramembranous Osteogenesis, Am J Anat 125:271-290.[CrossRef][Medline] [Order article via Infotrieve]
• Bonucci, E. (1970): Fine Structure and Histochemistry of "Calcifying Globules" in Epiphyseal Cartilage, Z Zellforsch Mikrosk Anat 103:192-217.[CrossRef][Medline] [Order article via Infotrieve]
• Bonucci, E. (1971): The Locus of Initial Calcification in Cartilage and Bone, Clin Orthop Relat Res 78:108-139.[CrossRef][Medline] [Order article via Infotrieve]
• Eisenmann, D.R. and Glick, P.L. (1972): Ultrastructure of Initial Crystal Formation in Dentin, J Ultrastruct Res 41:18-28.[CrossRef][Medline] [Order article via Infotrieve]
• Fortuna, R.; Anderson, H.C.; Carty, R.P.; and Sajdera, S.W. (1980): Enzymatic Characterization of the Matrix Vesicle Alkaline Phosphatase Isolated from Bovine Fetal Epiphyseal Cartilage, Calcif Tissue Int 30:217-225.[CrossRef][Medline] [Order article via Infotrieve]
• Fritz, P.J.; Morrison, W.J.; White, E.L.; and Vesell, E.S. (1970): Comparative Study of Methods for Quantitation of Lactate Dehydrogenase Isozymes, Anal Biochem 36:443-453.[CrossRef][Medline] [Order article via Infotrieve]
• Hosokawa, R.; Uchida, Y.; Fujiwara, S.; and Noguchi, T. (1988): Lactate Dehydrogenase Isoenzymes Are Present in Matrix Vesicles, J Biol Chem 263:10045-10047,[Abstract/Free Full Text]
• Landis, W.J.; Paine, M.C.; Hodgens, K.J.; and Glimcher, M.J. (1986): Matrix Vesicles in Embryonie Chick Bone: Consideration of their Identification, Number, Distribution, and Possible Effects on Calcification of Extracellular Matrices, J Ultrastruct Molec Res 95:142-163.
• Nelson, S.M. and Nora, L.S. (1990): Electron Microscopic Evaluation of the Occurrence of Matrix Vesicles in Cartilage, Anat Rec 227:397-404.[CrossRef][Medline] [Order article via Infotrieve]
• Noguchi, T. and Fujiwara, S. (1984): Developmental Profiles and Properties of Hepatic Peroxisomal Apo- and Mitochondrial Holoalanine:glyoxylate Aminotransferase during Chick Embryogenesis, J Biol Chem 259:14498-14504.[Abstract/Free Full Text]
• Noguchi, T. and Yaniashita, Y. (1987): The Rabbit Differs from Other Mammalian in the Tissue Distribution of Alkaline Phosphatase Isoenzymes, Biochim Biophys Res Commun 143:15-19.[CrossRef][Medline] [Order article via Infotrieve]
• Ohashi-Takeuchi, H.; Yamada, N.; Hosokawa, R.; and Noguchi, T. (1990): Vesicles with Lactate Dehydrogenase and without Alkaline Phosphatase Present in the Resting Zone of Epiphyseal Cartilage, Bioehem J 266:309-312.
• Oida, S.; Yamashita, Y.; and Sasaki, S. (1982): The Harvest of Homogeneous Bovine Odontoblasts and Their Alkaline Phosphatase Activity, Jpn J Oral Biol 24:811-819.
• Sisca, R.F. and Provenza, D.V. (1972): Initial Dentin Formation in Human Deciduous Teeth: An Electron Microscope Study, Calcif Tissue Res 9:1-16.[CrossRef][Medline] [Order article via Infotrieve]
• Watkins, E.L.; Stillo, J.V.; and Wuthier, R.E. (1980): Subcellutar Fraction of Epiphyseal Cartilage; Isolation of Matrix Vesicles and Profiles of Enzymes, Phospholipids, Calcium and Phosphate, Biochim Biophys Acta 391:289-304.
• Wuthier, R.E. (1989): Mechanism of de novo Mineral Formation By Matrix Vesicles, Conn Tissue Res 22:27-33.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 70, No. 11, 1444-1446 (1991)
DOI: 10.1177/00220345910700111001


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