|
Sign In to gain access to subscriptions and/or personal tools.
|
A Quantitative Analysis of Mineral Loss and Shrinkage of in vitro Demineralized Human Root Surfaces
J.M. Ten Cate
Department of Cariology & Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands
B. Nyvad
Department of Oral Anatomy, Dental Pathology, and Operative Dentistry, Royal Dental College Aarhus, Aarhus, Denmark
Y.M. Van De Plassche-Simons
Department of Cariology & Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA Amsterdam, The Netherlands
O. Fejerskov
Department of Oral Anatomy, Dental Pathology, and Operative Dentistry, Royal Dental College Aarhus, Aarhus, Denmark
Demineralization of dentin specimens proceeds at a faster rate than that of enamel. Although this is generally accepted, a quantification of the rate of formation of root lesions is hampered by the shrinkage of the lesions when these are dried prior to microradiographic analysis.' This leads to a significant underestimation of the lesion depth and total mineral loss. The aim of this paper was to quantitate the rate of mineral loss during root lesion formation in vitro and to determine the shrinkage of root specimens as a result of drying. Unerupted roots of human teeth were subjected to a demineralizing system of 0.1 mol/L lactate buffer (pH = 4.8) with 0.2 mmol/L methanehydroxydiphosphonate during four, 11, 22, and 44 days. The root lesions were assessed by quantitative microradiography. The demineralizing solutions were analyzed to determine the amounts of root tissue dissolved. A comparison of these two sets of data showed that, with the demineralizing system used, root lesions may shrink up to 62%. Fixation of the specimens in fixative did not affect this shrinkage. Chemical analysis showed that mineral loss proceeded linearly with time. From the data-sets of this study, a model was developed to compensate for the shrinkage in the dentin specimens. In this way, it was possible to calculate the lesion depth at four demineralization times as being 130, 220, 320, and 530 µm, respectively. These values were in agreement with a microscopic determination of the lesion depth.
REFERENCES
- Almqvist, H.; Wefel, J.S.; Lagerlof, F.; Ekstrand, J.; and Henrikson, C.O. (1988): In vitro Root Caries Progression Measured by 125I Absorptiometry: Comparison with Chemical Analysis, J Dent Res 67:1217-1220.[Abstract/Free Full Text]
- Angmar, B.; Carlstrom, P.; and Glas, J.E. (1963): Studies on the Ultrastructure of Dental Enamel, IV. The Mineralization of Normal Human Enamel, J Ultrastruct Res 8:12-23.[Medline]
[Order article via Infotrieve]
- Arends,J.; Christoffersen, J.; Ruben, J.; and Christoffersen, M.R. (1987): Lesion Progress in Dentine and the Role of Fluoride. In: Dentine and Dentine Reactions in the Oral Cavity, A. Thylstrup, S.A. Leach, and V. Qvist, Eds., Oxford: IRL Press, pp. 117-125.
- Boyde, A. (1978): Pros and Cons of Critical Point Drying for SEM, Scan Electron Microsc 11:303-314.
- Featherstone, J.D.B.; McIntyre, J.M.; and Fu, J. (1987): Physicochemical Aspects of Root Caries Progression. In: Dentine and Dentine Reactions in the Oral Cavity, A. Thylstrup, S.A. Leach, and V. Qvist, Eds., Oxford: IRL Press, pp. 127-137.
- Hayat, M.A. (1970): Principles and Techniques of Electron Microscopy, Biological Applications, Vol. 1, New York: Van Nostrand Reinhold Co., pp. 65-81.
- Hoppenbrouwers, P.M.M.; Driessens, F.C.M.; and Borggreven, J.M.P.M. (1986): The Vulnerability of the Unexposed Human Dental Roots to Demineralization, J Dent Res 65:955-958.[Abstract/Free Full Text]
- Hoppenbrouwers, P.M.M.; Driessens, F.C.M.; and Borggreven, J.M.P.M. (1987): The Mineral Solubility of Human Tooth Roots, Arch Oral Biol 32:319-322.[CrossRef][Medline]
[Order article via Infotrieve]
- Karnovsky,M.J.( 1965):AFormaldehyde-GlutaraldehydeFixativeofHigh Osmolality for Use in Electron Microscopy, J Cell Biol 26:137A-138A.[CrossRef]
- Mellberg, J.R. and Sanchez, M. (1986): Remineralization by a Monofluorophosphate Dentifrice in vitro of Root Dentin Softened by Artificial Caries, J Dent Res 65:959-962.[Abstract/Free Full Text]
- Nyvad, B.; Ten Cate, J.M.; and Fejerskov, O. (1989): Microradiography of Experimental Root Surface Caries in Man, Caries Res 23:218-224.[Medline]
[Order article via Infotrieve]
- Ogaard, B.; Rølla, G.; and Arends, J. (1988): In vivo Progress of Enamel and Root Surface Lesions under Plaque as a Function of Time, Caries Res 22:302-305.[Medline]
[Order article via Infotrieve]
- Phankosol, P.; Ettinger, R.L.; Hicks, M.J.; and Wefel, J.S. (1985): Histopathology of the Initial Lesion of the Root: An in vitro Study, JDent Res 64:804-809.
- Wefel, J.S.; Clarkson, B.H.; and Heilman, J.R. (1987): Histology of Root Surface Caries. In: Dentine and Dentine Reactions in the Oral Cavity, A. Thylstrup, S.A. Leach, and V. Qvist, Eds., Oxford: IRL Press, pp. 139-146.
Journal of Dental Research, Vol. 70, No. 10,
1371-1374 (1991)
DOI: 10.1177/00220345910700101101
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Nyvad, J.M. Ten Cate, and O. Fejerskov
Arrest of Root Surface Caries in situ
Journal of Dental Research,
December 1, 1997;
76(12):
1845 - 1853.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Arends, J.L. Ruben, and D. Inaba
Major Topics in Quantitative Microradiography of Enamel and Dentin: R Parameter, Mineral Distribution Visualization, and Hyper-Remineralization
Advances in Dental Research,
November 1, 1997;
11(4):
403 - 414.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.J.M. Damen, R.A.M. Exterkate, and J.M. Ten Cate
Reproducibility of TMR for the Determination of Longitudinal Mineral Changes in Dental Hard Tissues
Advances in Dental Research,
November 1, 1997;
11(4):
415 - 419.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.S. Wefel
In Situ Caries Models: Reaction Paper
Advances in Dental Research,
November 1, 1995;
9(3):
231 - 234.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.M. ten Cate and R.N.B. van Duinen
Hypermineralization of Dentinal Lesions Adjacent to Glass-ionomer Cement Restorations
Journal of Dental Research,
June 1, 1995;
74(6):
1266 - 1271.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.H. Kinney, M. Balooch, D.L. Haupt Jr, S.J. Marshall, and G.W. Marshall Jr
Mineral Distribution and Dimensional Changes in Human Dentin during Demineralization
Journal of Dental Research,
May 1, 1995;
74(5):
1179 - 1184.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.M. Ten Cate
In Situ Models, Physico-Chemical Aspects
Advances in Dental Research,
July 1, 1994;
8(2):
125 - 133.
[Abstract]
[PDF]
|
|
|
|
|
