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Journal of Dental Research
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The Role of Leukotriene B4 in Neutrophil Infiltration in Experimentally-induced Inflammation of Rat Tooth Pulp

T. Okiji

Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan, Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

I. Morita

Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

I. Sunada

Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

S. Murota

Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

Inflammation was induced in rat dental pulp by applying bacterial lipopolysaccharide (LPS). Extirpated tissue samples from inflamed pulps were incubated in vitro in a Krebs buffer containing Ca2+ ionophore A23187, and leukotriene (LT) B4 released into the medium was determined by radio-immunoassay. Production of LTB4 could be detected three to 24 h after the application of LPS and showed a maximum at 12 h. Histologically, marked infiltration of neutrophils, but not other leukocytes, was characteristically observed in the LPS-applied pulps, and the temporal change in neutrophil infiltration was almost parallel, but somewhat more delayed than LTB4 production. When BW755C, a dual inhibitor of cyclo-oxygenase and lipoxygenase, was given to the animals before the application of LPS, both the production of LTB4 and the number of infiltrated neutrophils were significantly decreased, whereas administration of indomethacin had no effect. These results suggest that LTB4 may be involved in neutrophil infiltration in pulpal inflammation. It was also suggested that a major early source of LTB4 in experimental pulpitis was leukocytes, primary neutrophils, because the synthesis of LTB4 in the inflamed pulp was diminished by depletion of circulating leukocytes with cyclophosphamide prior to the application of LPS.

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Journal of Dental Research, Vol. 70, No. 1, 34-37 (1991)
DOI: 10.1177/00220345910700010501
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