This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kim, S.K. Articles by Allen, E.D. Search for Related Content PubMed PubMed Citation Articles by Kim, S.K. Articles by Allen, E.D. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB STREPTOZOTOCIN Social Bookmarking                         What's this?

Effects of Diabetes and Insulin on -amylase Messenger RNA Levels in Rat Parotid Glands

Research Service, VA Medical Center, Ann Arbor, Michigan 48105, Departments of Anatomy and Cell Biology, Medical School, Biologic and Material Sciences, School of Dentistry, University of Michigan, Ann Arbor

L.M. Cuzzort

Departments of Anatomy and Cell Biology, Medical School

R.K. McKean

Departments of Anatomy and Cell Biology, Medical School

E.D. Allen

Research Service, VA Medical Center, Ann Arbor, Michigan 48105

Previous studies have shown that amylase levels are reduced significantly in the pancreas and parotid gland of diabetic rats and that insulin reverses this effect and increases the secretory protein levels. In the pancreas, these changes in amylase protein levels are accompanied by parallel changes in amylase mRNA levels. In the present study, the effects of diabetes and subsequent insulin treatments on contents (per cell) of amylase protein and its mRNA in parotid glands were compared in rats rendered diabetic with an injection of a beta-cell toxin, streptozotocin (STZ). Both amylase protein and its mRNA contents were reduced significantly in diabetic rats, compared with control rats, and this reduction was reversed following insulin injections of diabetic rats. In insulin-injected diabetic rats, amylase protein contents increased before a detectable increase in amylase mRNA levels was seen. The mRNA contents of a non-secretory protein, actin, did not change during diabetogenesis or subsequent insulin treatments. The reductions in parotid contents of amylase and its mRNA in diabetic rats and the reversal of these changes by insulin are similar to those changes that occur in the pancreas under the same conditions. However, the magnitude of these changes in parotid glands was much smaller than in the pancreas, and the effect of insulin on amylase mRNA synthesis was not as immediate as in the latter gland.

REFERENCES

• Anderson, L.C. (1983): Effects of Alloxan Diabetes and Insulin in vivo on Parotid Gland, Am J Physiol 245:G431-G437.[Medline] [Order article via Infotrieve]
• Anderson, L.C. and Johnson, D.A. (1981): Effects of Alloxan Diabetes on Rat Parotid Gland and Saliva, Comp Biochem Physiol B 70:725-730.[CrossRef]
• Ben Abdeljlil, A.; Palla, J.C.; and Desnuelle, P. (1965): Effect of Insulin on Pancreatic Amylase and Chymotrypsinogen, Biochem Biophys Res Commun 18:71-75.[CrossRef][Medline] [Order article via Infotrieve]
• Bernfeld, P. (1955): Amylases, Alpha and Beta, Meth Enzymol 1:149-158.[Medline] [Order article via Infotrieve]
• Burton, K. (1955): A Study of the Conditions and Mechanism of Diphenylamine Reaction for the Colorimetric Estimation of Deoxyribonucleic Acid, Biochem J 162:315-323.
• Chirgwin, J.M.; Pryzybyla, A.E.; MacDonald, R.J.; and Rutter, W.J. (1979): Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease, Biochem 18:5294-5299.[CrossRef][Medline] [Order article via Infotrieve]
• Christophe, J.; Camus, J.; Deschodt-Lanckman, M.; Rathe, J.; Robbereccht, P.; Vandermeers-Pieret, M.C.; and Vandermeers, A. (1971): Factors Regulating Biosynthesis, Intracellular Transport and Secretion of Amylase and Lipase in the Rat Pancreas, Horm Metab Res 3:393-403.[Medline] [Order article via Infotrieve]
• Hagenbuchle, 0.; Bovey, R.; and Young, R.A. (1980): Tissue-specific Expression of Mouse Alpha-Amylase Genes: Nucleotide Sequence of Isozyme mRNAs from Pancreas and Salivary Gland, Cell 21:179-187.[CrossRef][Medline] [Order article via Infotrieve]
• Hagenbuchle, 0.; Tosi, M.; Schibler, U.; Bovey, R.; Wellauer, P.K.; and Young, R.A. (1981): Mouse Liver and Salivary Gland -Amylase mRNA's Differ only in 5' Non-translated Sequences, Nature 289:643-646.[CrossRef][Medline] [Order article via Infotrieve]
• Hand, A.R. and Weiss, R.E. (1984): Effects of Streptozotocin Diabetes on the Rat Parotid Gland, Lab Invest 51:429-440.
• Jonas, A.M.; Craft, J.; Black, C.L.; Bhatt, P.N.; and Hilding, D. (1969): Sialodacryoadenitis in the Rat. A Light and Electron Microscopic Study, Arch Pathol 88:613-622.[Medline] [Order article via Infotrieve]
• Kim, S.K. (1981): Age-related Changes in the Cellular Level of Amylase and Protein Synthesis in the Rat Parotid Gland, J Dent Res 60:738-749.[Abstract/Free Full Text]
• Kim, S.K.; Weinhold, P.A.; Han, S.S.; and Wagner, D.J. (1980): Age-related Decline in Protein Synthesis in the Rat Parotid Gland, Exp Gerontol 15:71-85.
• Kim, S.K.; Weinhold, P.A.; Calkins, D.W.; and Hartog, V.W. (1981): Comparative Studies of the Age-related Changes in Protein Synthesis in the Rat Pancreas and Parotid Gland, Exp Gerontol 16:91-100.[Medline] [Order article via Infotrieve]
• Korc, M.; Iwamoto, Y.; Sankaran, H.; Williams, J.A.; and Goldfine, I.D. (1981a): Insulin Action in Pancreatic Acini from Streptozotocin-treated Rats. I. Stimulation of Protein Synthesis, Am J Physiol 240:G56-G62.
• Korc, M.; Owerbach, D.; Quinto, C.; and Rutter, W.J. (1981b): Pancreatic Islet-Acinar Cell Interaction: Amylase Messenger RNA Levels are Determined by Insulin, Science 213:351-353.[Abstract/Free Full Text]
• Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
• Maniatis, T.; Fritsch, E.F.; and Sanibrook, J. (1982): Molecular Cloning, A Laboratory Manual, New York, NY: Cold Spring Harbor Lab.
• McPherson, M.A. and Hales, C.N. (1978): Control of Amylase Biosynthesis and Release in the Parotid Gland of the Rat, Biochem J 176:855-863.[Medline] [Order article via Infotrieve]
• Okabayashi, Y.; Moessner, J.; Logdson, C.D.; Goldfine, I.D.; and Williams, J.A. (1987): Insulin and Other Stimulants have Nonparallel Translational Effects on Protein Synthesis, Diabetes 36:1054-1060.[Abstract]
• Osborn, L; Rosenberg, M.P.; Keller, S.A.; and Meisler, M.H. (1987): Tissue-specific and Insulin-dependent Expression of a Pancreatic Amylase Gene in Transgenic Mice, Mol Cell Biol 7:326-334.[Abstract/Free Full Text]
• Osborn, L; Rosenberg, M.P.; Keller, S.A.; Ting, C.-N.; and Meisler, M.H. (1988): Insulin Response of a Hybrid Amylase/CAT Gene in Transgenic Mice, J Biol Chem 263:16519-16522.[Abstract/Free Full Text]
• Palla, J.C.; Ben Abdeljlil, A.; and Desnuelle, P. (1967): Comparative Study of the Control of Amylase Biosynthesis in Rat Pancreas and Parotid Glands, Biochim Biophys Acta 136:563-565.
• Schibler, U.; Hagenbuchle, 0.; Wellauer, P.K.; and Pittet, A.C. (1983): Two Promoters of Different Strengths Control the Transcription of the Mouse Alpha-Amylase Gene Amy-1 in the Parotid Gland and the Liver, Cell 33:501-508.[CrossRef][Medline] [Order article via Infotrieve]
• Schick, J.; Verspohl, R.; Kern, H.; and Scheele, G. (1984a): Two Distinctive Adaptive Responses in the Synthesis of Exocrine Pancreas Results in Coordinate and Anticoordinate Regulation of Protein Synthesis, Am J Physiol 247:G611-G616.
• Schick, J.; Kern, H.; and Scheele, G. (1984b): Hormonal Stimulation in the Exocrine Pancreas Results in Coordinate and Anticoordinate Regulation of Protein Synthesis, J Cell Biol 99:1569-1574.[Abstract/Free Full Text]
• Sierra, F.; Pittet, A.; and Schibler, U. (1986): Different Tissue-specific Expression of the Amylase Gene Amy-1 in Mice and Rats, Mol Cell Biol 6:4067-4076.[Abstract/Free Full Text]
• Snook, J.T. (1968): Effect of Diet, Adrenalectomy, Diabetes , and Actinomycin D on Exocrine Pancreas, Am J Physiol 215:1329-1333.
• Soling, H.D. and Unger, K.O. (1972): The Role of Insulin in the Regulation of -Amylase Synthesis in the Rat Pancreas, Eur J Clin Invest 2:199-212.[Medline] [Order article via Infotrieve]
• Wiebauer, K.; Gumucio, D.L.; Jones, J.M.; Caldwell, R.M.; Hartle, H.T.; and Meisler, M.H. (1985): A 78-kilobase Region of Mouse Chromosome 3 Contains Salivary and Pancreatic Amylase Genes and a Pseudogene, Proc Natl Acad Sci USA 82:5446-5449.[Abstract/Free Full Text]
• Williams, J.A. and Goldfine, I.D. (1986): The Insulin-acinar Relationship. In: The Exocrine Pancreas: Biology, Pathology, and Diseases, V.L.W. Go, Ed., New York: Raven Press, pp. 347-360.
• Young, R.A.; Hagenbuchle, O.; and Schibler, U. (1981): A Single Mouse Alpha-Amylase Gene Specifies Two Different Tissue-specific mRNAs, Cell 23:451-458.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 8, 1500-1504 (1990)
DOI: 10.1177/00220345900690081001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kim, S.K. Articles by Allen, E.D. Search for Related Content PubMed PubMed Citation Articles by Kim, S.K. Articles by Allen, E.D. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB STREPTOZOTOCIN Social Bookmarking                         What's this?