Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Loos, B.G.
Right arrow Articles by Dickinson, D.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loos, B.G.
Right arrow Articles by Dickinson, D.P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Genetic Heterogeneity of Porphyromonas (Bacteroides) gingivalis by Genomic DNA Fingerprinting

B.G. Loos

Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, New York 14214

D. Mayrand

Groupe de Recherche en Ecologie Buccale, Département de Biochimie, Faculté des Sciences et de Genie, Université Laval, Quebec City, Quebec, Canada GlK 7P4

R.J. Genco

Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, New York 14214

D.P. Dickinson

Department of Biological Chemistry, University of Texas Health Science Center, Dental Branch, Houston, Texas 77025

This study describes the use of total genomic DNA fingerprinting with the use of restriction endonucleases to characterize clinical isolates of Porphyromonas gingivalis (Bacteroides gingivalis) obtained from patients with periodontitis or with root-canal infections. The majority of independent isolates had a unique DNA fingerprint, indicating extensive genetic heterogeneity within this species. Twenty-nine distinct DNA fingerprints were found among the 33 isolates investigated. This is in contrast to biotyping and serotyping, where only one type and three types, respectively, have been reported. The observed heterogeneity indicates that DNA fingerprinting is a sensitive measure of genetic dissimilarity between P. gingivalis isolates and is able to characterize individual isolates. These results have ecological implications, indicating that there is considerable natural diversity in the global population of P. gingivalis, and that there are likely to be relatively large numbers of genetically distinct clonal lines. Furthermore, DNA fingerprinting is a sensitive and powerful tool for longitudinal and cross-sectional epidemiological studies. This technique provides far greater discrimination between isolates than either biotyping or serotyping, and will be most helpful in, for example, the analysis of distribution of clonal lines within one periodontal patient, or the analysis of the transmission to and turnover of strain populations within a patient population, since the probability of two strains with the same DNA fingerprint being found by chance is small.

REFERENCES

  • Allan, I.; Loeb, M.R.; and Moxon, E.R. (1987): Limited Genetic Diversity of Haemophilus influenzae (type b), Microb Pathog 2:139-145.[CrossRef][Medline] [Order article via Infotrieve]
  • Barua, P.K.; Dyer, D.W.; and Neiders, M.E. (1989): Response of Bacteroides gingivalis to Iron Limitation, J Dent Res 68:358, Abst. No. 1415.
  • Caufield, P.W. and Walker, T.M. (1989): Genetic Diversity within Streptococcus mutans Evident from Chromosomal DNA Restriction Fragment Polymorphisms, J Clin Microbiol 27:274-278.[Abstract/Free Full Text]
  • Christersson, L.A.; Zambon, J.J.; Dunford, R.G.; Grossi, S.G.; and Genco, R.J. (1989): Specific Subgingival Bacteria and Diagnosis of Gingivitis and Periodontitis, J Dent Res 68:1633-1639.
  • Coykendall, A.L.; Kaczmarek, F.S.; and Slots, J. (1980): Genetic Heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) (Finegold and Bames 1977) (Approved list, 1980) and Proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov., Int J Syst Bacteriol 30:559-564.[Abstract/Free Full Text]
  • Denning, D.W.; Baker, C.J.; Troup, N.J.; and Tompkins, L.S. (1989): Restriction Endonuclease Analysis of Human and Bovine Group B Streptococci for Epidemiological Study, J Clin Microbiol 27:1352-1356.[Abstract/Free Full Text]
  • Dickinson, D.P.; Rivoli, P.S.; Brewster, J.M.; and Genco, R.J. (1987): Plasmids in Black-pigmented Members of the Genus Bacteroides, J Dent Res 66:223, Abst. No. 933.
  • Dickinson, D.P.; Loos, B.G.; Dryja, D.M.; and Bernstein, J.M. (1988): Restriction Fragment Mapping of Branhamella catarrhalis: A New Tool to Study the Epidemiology of this Middle Ear Pathogen, J Infect Dis 158:205-208.[Medline] [Order article via Infotrieve]
  • Dzink, J.L.; Socransky, S.S.; and Haffajee, A.D. (1988): The Predominant Cultivable Microbiota of Active and Inactive Lesions of Destructive Periodontal Diseases, J Clin Periodontol 15:316-323.[CrossRef][Medline] [Order article via Infotrieve]
  • Ebersole, J.L.; Taubman, M.A.; Smith, D.J.; and Frey, D.E. (1986): Human Immune Responses to Oral Microorganisms: Patterns of Systemic Antibody Levels to Bacteroides Species, Infect Immun 51:507-513.[Abstract/Free Full Text]
  • Fisher, J.G.; Zambon, J.J.; and Genco, R.J. (1987): Identification of Serogroup-specific Antigens among Bacteroides gingivalis Components, J Dent Res 66:222, Abst. No. 927.
  • Fisher, J.G. and Zambon, J.J. (1989): The Effect of Hemin Concentration on the Outer Membrane of Bacteroides gingivalis, J Dent Res 68:358, Abst. No. 1414.
  • Goodson, J.M.; Tanner, A.C.R.; Haffajee, A.D.; Sor-Berger, G.C.; and Socransky, S.S. (1982): Patterns of Progression and Regression of Advanced Destructive Periodontal Disease, J Clin Periodontol 9:427-481.
  • Grenier, D. and Mayrand, D. (1987): Selected Characteristics of Pathogenic and Nonpathogenic Strains of Bacteroides gingivalis, J Clin Microbiol 25:738-740.[Abstract/Free Full Text]
  • Grothues, D.; Koopmann, U.; Von Der Hardt, H.; and Tummler, B. (1988): Genome Fingerprinting of Pseudomonas aeruginosa Indicates Colonization of Cystic Fibrosis Siblings with Closely Related Strains, J Clin Microbiol 26:1973-1977.[Abstract/Free Full Text]
  • Haapasalo, M.; Ranta, H.; Ranta, K.; and Shah, H. (1986): Black-pigmented Bacteroides spp. in Human Apical Periodontitis, Infect Immun 53:149-153.[Abstract/Free Full Text]
  • Haffajee, A.D.; Socransky, S.S.; and Goodson, J.M. (1983): Comparison of Different Data Analyses for Detecting Changes in Attachment Levels, J Clin Periodontol 10:513-521.
  • Kulkarni, G.V.; Chan, K.H.; and Sandham, H.J. (1989): An Investigation into the Use of Restriction Endonuclease Analysis for the Study of Transmission of Mutans Streptococci, J Dent Res 68:1155-1161.[Abstract/Free Full Text]
  • Laliberte, M. and Mayrand, D. (1983): Characterization of Black-pigmented Bacteroides Strains Isolated from Animals, JAppl Microbiol 55:247-252.
  • Langenberg, W.; Rauws, E.A.J.; Widjojokusumo, A.; Tytgat, G.N.J.; and Zanen, H.C. (1986): Identification of Campylobacter pyloridis Isolates by Restriction Endonuclease DNA Analysis, J Clin Microbiol 24:414-417.[Abstract/Free Full Text]
  • Laughon, B.E.; Syed, S.A.; and Loesche, W.J. (1982): API ZYM System for Identification of Bacteroides spp., Capnocytophaga spp., and Spirochetes of Oral Origin, J Clin Microbiol 15:97-102.[Abstract/Free Full Text]
  • Loesche, W.J.; Syed, S.A.; Schmidt, E.; and Morrison, E.C. (1985): Bacterial Profiles of Subgingival Plaques in Periodontitis, J Periodontol 56:447-456.[Medline] [Order article via Infotrieve]
  • Loos, B.G.; Beltran, P.; Genco, R.J.; and Selander, R.K. (1989a): Genetic Relationships among Bacteroides gingivalis Isolates, J Dent Res 68:256, Abst. No. 598.
  • Loos, B.G.; Bernstein, J.M.; Dryja, D.M.; Murphy, T.F.; and Dickinson, D.P. (1989b): Determination of the Epidemiology and Transmission of Nontypeable Haemophilus influenzae in Children with Otitis Media by Comparison of Total Genomic DNA Restriction Fingerprints, Infect Immun 57:2751-2757.[Abstract/Free Full Text]
  • Loos, B.G.; Genco, R.J.; and Dickinson, D.P. (1989c): Epidemiology of Bacteroides gingivalis using DNA Restriction Fragment Pattern Analyses, J Dent Res 68:985, Abst. No. 944.
  • Maniatis, T.; Fritsch, E.F.; and Sambrook, J. (1982): Molecular Cloning: a Laboratory Manual, Cold Spring Harbor, NY: Cold Harbor Spring Laboratory.
  • Mayrand, D. and Holt, S.C. (1988): Biology of Asaccharolytic Black-pigmented Bacteroides Species, Microbiol Rev 52:134-152.[Free Full Text]
  • Moore, W.E.C.; Ranney, R.R.; and Holdeman, L.V. (1982): Subgingival Microflora in Periodontal Disease: cultural studies. In: Host-parasite Interactions in Periodontal Disease, R.J. Genco and S. Mergenhagen, Eds., Washington, DC: American Society for Microbiology, pp. 13-26.
  • Mouton, C.; Hammond, P.G.; Slots, J.; and Genco, R.J. (1981): Serum Antibodies to Oral Bacteroides asaccharolyticus (Bacteroidesgingivalis): Relationship to Age and Periodontal Disease, Infect Immun 31:182-192.[Abstract/Free Full Text]
  • Musser, J.M.; Granoff, D.M.; Pattison, P.E.; and Selander, R.K. (1985): A Population Genetic Framework for the Study of Invasive Diseases Caused by Serotype b Strains of Haemophilus influenzae, Proc Natl Acad Sci USA 82:5078-5082.[Abstract/Free Full Text]
  • Ochman, H. and Selander, R.K. (1984): Evidence for Clonal Population Structure in Escherichia coli, Proc Natl Acad Sci USA 81:198-201.[Abstract/Free Full Text]
  • Ørskov, F. and Ørskov, I. (1983): Summary of a Workshop on the Clone Concept in the Epidemiology, Taxonomy, and Evolution of the Enterobacteriaceae and Other Bacteria, J Infect Dis 148:346-357.[Medline] [Order article via Infotrieve]
  • Parent, R.; Mouton, C.; Lamonde, L.; and Bouchard, D. (1986): Human and Animal Serotypes of Bacteroides gingivalis Defined by Crossed Immunoelectrophoresis, Infect Immun 51:909-918.[Abstract/Free Full Text]
  • Sako, K.; Takazoe, I.; and Okuda, K. (1988): Isolation and Characterization of Plasmid DNA from Bacteroides Strains Isolated from the Human Oral Cavity, Oral Microbiol Immunol 3:72-76.[CrossRef][Medline] [Order article via Infotrieve]
  • Scherer, S. and Stevens, D.A. (1987): Application of DNA Typing Methods to Epidemiology and Taxonomy of Candida Species, J Clin Microbiol 25:675-679.[Abstract/Free Full Text]
  • Selander, R.K.; Caugant, D.A.; Ochman, H.; Musser, J.M.; Gilmour, M.N.; and Whittam, T.S. (1986): Methods of Multilocus Enzyme Electrophoresis for Bacterial Population Genetics and Systematics, Appl Environ Microbiol 51:873-884.[Free Full Text]
  • Selander, R.K.; Musser, J.M.; Caugant, D.M.; Gilmour, M.N.; and Whittam, T.S. (1987): Population Genetics of Pathogenic Bacteria. Mini-review, Microb Pathog 3:1-7.[CrossRef][Medline] [Order article via Infotrieve]
  • Shah, H.N. and Collins, M.D. (1988): Proposal for Reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis and Bacteroides endodontalis in a New Genus, Porphyromonas, Int J Syst Bacteriol 38:128-131.
  • Slots, J. and Gibbons, R.J. (1978): Attachment of Bacteroides melaninogenicus Subsp. asaccharolyticus to Oral Surfaces and its Possible Role in Colonization of the Mouth and of Periodontal Pockets, Infect Immun 19:254-264.[Abstract/Free Full Text]
  • Slots, J. (1981): Enzymatic Characterization of Some Oral and Nonoral Gram-negative Bacteria with the API ZYM System, J Clin Microbiol 14:288-294.[Abstract/Free Full Text]
  • Slots, J. and Reynolds, H.S. (1982): Long-wave UV Light Fluorescence for Identification of Black-pigmented Bacteroides spp., J Clin Microbiol 16:1148-1151.[Abstract/Free Full Text]
  • Slots, J.; Bragd, L.; Wikstrom, M.; and Dahlen, G. (1986): The Occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in Destructive Periodontal Disease in Adults, J Clin Periodontol 13:570-577.[CrossRef][Medline] [Order article via Infotrieve]
  • Slots, J. and Listgarten, M.A. (1988): Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus acinomycetemcomitans in Human Periodontal Diseases, J Clin Periodontol 15:85-93.[CrossRef][Medline] [Order article via Infotrieve]
  • Syed, S.A. (1980): Characteristics of Bacteroides asaccharolyticus from Dental Plaque of Beagle Dogs, J Clin Microbiol 11:522-526.[Abstract/Free Full Text]
  • Tanner, A.C.R.; Socransky, S.S.; and Goodson, J.M. (1984): Microbiota of Periodontal Pockets Losing Crestal Alveolar Bone, J Periodont Res 19:279-291.[CrossRef][Medline] [Order article via Infotrieve]
  • Tew, J.G.; Marshall, D.R.; Moore, W.E.C.; Best, A.M.; Palcanis, K.G.; and Ranney, R.R. (1985): Serum Antibody Reactive with Predominant Organisms in the Subgingival Flora of Young Adults with Generalized Severe Periodontitis, Infect Immun 48:303-311.[Abstract/Free Full Text]
  • Van Winkelhoff, A.J.; Carlee, A.W.; and De Graaff, J. (1985): Bacteroides endodontalis and Other Black-pigmented Bacteroides Species in Odontogenic Abscesses, Infect Immun 49:494-497.[Abstract/Free Full Text]
  • Van Winkelhoff, A.J.; Van Steenbergen, T.J.M.; and De Graaff, J. (1988): The Role of Black-pigmented Bacteroides in Human Oral Infections, J Clin Periodontol 15:145-155.[CrossRef][Medline] [Order article via Infotrieve]
  • Wachsmuth, K. (1986): Molecular Epidemiology of Bacterial Infections: Examples of Methodology and of Investigations of Outbreaks, Rev Infect Dis 8:682-692.[Medline] [Order article via Infotrieve]
  • White, D. and Mayrand, D. (1981): Association of Oral Bacteroides with Gingivitis and Adult Periodontitis, J Periodont Res 16:259-265.[CrossRef][Medline] [Order article via Infotrieve]
  • Wilson, K. (1987): Preparation of Genomic DNA from Bacteria. In: Current Protocols in Molecular Biology, F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J. A. Smith, and K. Struhl, Eds., New York, NY: Greene Publishing Associates and Wiley-Interscience, John Wiley & Sons, pp. 2.4.1-2.4.5.
  • Wren, B.W. and Tabaqchali, S. (1987): Restriction Endonuclease DNA Analysis of Clostridium difficile, J Clin Microbiol 25:2402-2404.[Abstract/Free Full Text]
  • Zambon, J.J.; Reynolds, H.S.; and Slots, J. (1981): Black-pigmented Bacteroides sp. in the Human Oral Cavity, Infect Immun 32:198-203.[Abstract/Free Full Text]
  • Zambon, J.J.; Sunday, G.J.; and Smutko, J.S. (1990): Molecular Genetic Analysis of Actinobacillus actinomycetemcomitans Ecology, J Periodontol 61:75-80.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 8, 1488-1493 (1990)
DOI: 10.1177/00220345900690080801
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
C. O. Igboin, A. L. Griffen, and E. J. Leys
Porphyromonas gingivalis Strain Diversity
J. Clin. Microbiol., October 1, 2009; 47(10): 3073 - 3081.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. Koehler, H. Karch, T. Beikler, Thomas. F. Flemmig, S. Suerbaum, and H. Schmidt
Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination
Microbiology, September 1, 2003; 149(9): 2407 - 2415.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. V. Califano, T. Kitten, J. P. Lewis, F. L. Macrina, R. D. Fleischmann, C. M. Fraser, M. J. Duncan, and F. E. Dewhirst
Characterization of Porphyromonas gingivalis Insertion Sequence-Like Element ISPg5
Infect. Immun., September 1, 2000; 68(9): 5247 - 5253.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. W. Rumpf, A. L. Griffen, and E. J. Leys
Phylogeny of Porphyromonas gingivalis by Ribosomal Intergenic Spacer Region Analysis
J. Clin. Microbiol., May 1, 2000; 38(5): 1807 - 1810.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
E. J. Leys, J. H. Smith, S. R. Lyons, and A. L. Griffen
Identification of Porphyromonas gingivalis Strains by Heteroduplex Analysis and Detection of Multiple Strains
J. Clin. Microbiol., December 1, 1999; 37(12): 3906 - 3911.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
R. W. Rumpf, A. L. Griffen, B.-G. Wen, and E. J. Leys
Sequencing of the Ribosomal Intergenic Spacer Region for Strain Identification of Porphyromonas gingivalis
J. Clin. Microbiol., August 1, 1999; 37(8): 2723 - 2725.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
J. P. Lewis and F. L. Macrina
Localization of HArep-Containing Genes on the Chromosome of Porphyromonas gingivalis W83
Infect. Immun., May 1, 1999; 67(5): 2619 - 2623.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. A. Hanley, J. Aduse-Opoku, and M. A. Curtis
A 55-Kilodalton Immunodominant Antigen of Porphyromonas gingivalis W50 Has Arisen via Horizontal Gene Transfer
Infect. Immun., March 1, 1999; 67(3): 1157 - 1171.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
R. J. Lamont and H. F. Jenkinson
Life Below the Gum Line: Pathogenic Mechanisms of Porphyromonas gingivalis
Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1244 - 1263.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
G. H.W. Bowden and I. R. Hamilton
Survival of Oral Bacteria
Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(1): 54 - 85.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
G. Bowden, J. Johnson, and C. Schachtele
Characterization of Actinomyces with Genomic DNA Fingerprints and rRNA Gene Probes
Journal of Dental Research, August 1, 1993; 72(8): 1171 - 1179.
[Abstract] [PDF]

Home page
 JDRHome page
B.G. Loos and D.W. Dyer
Restriction Fragment Length Polymorphism Analysis of the Fimbrillin Locus, fimA, of Porphyromonas gingivalis
Journal of Dental Research, May 1, 1992; 71(5): 1173 - 1181.
[Abstract] [PDF]

Home page
 JDRHome page
B.G. Loos, A.J. Van Winkelhoff, R.G. Dunford, R.J. Genco, J. De Graaff, D.P. Dickinson, and D.W. Dyer
A Statistical Approach to the Ecology of Porphyromonas gingivalis
Journal of Dental Research, February 1, 1992; 71(2): 353 - 358.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Loos, B.G.
Right arrow Articles by Dickinson, D.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loos, B.G.
Right arrow Articles by Dickinson, D.P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?