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Journal of Dental Research
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Plaque Fluid as a Bacterial Milieu

W.M. Edgar

School of Dentistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, England

S.M. Higham

School of Dentistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, England

Studies of the extracellular, free concentrations of substrates, growth factors, inhibitors, and end-products of metabolism to which the intact plaque microflora is exposed in situ can assist in the understanding of factors controlling plaque pathogenicity. Information is becoming increasingly available from analysis of fluid separated by centrifugation of plaques collected at various intervals after an intra-oral pulse of dietary or experimental substrate, or different procedures or treatments having cariostatic potential. Such analytical results give more information than those obtained by analysis of aqueous or other extracts, because they yield values of substrate concentration representing those occurring at the bacterial cell surface.

The largest body of information concerns extracellular levels of acid end-products of sugar catabolism in relation to food quality or sequence, and of amino acids and other products of nitrogen metabolism, in relation to studies of the detailed metabolic events of the Stephan curve, and of the demineralizing effect of the plaque environment. Areas where little information is available and which merit further study include plaque clearance of salivary and other components with anti-caries activity (e.g., antibodies, enzymes, fluorides, cations, other antimicrobials, etc.), and substrate concentrations to determine gradients for diffusion into and out of plaque.

REFERENCES

  • Birkeland, J.M. and Charlton, G. (1976): Effect of pH on the Fluoride Ion Activity of Plaque, Caries Res 10:72-80.[Medline] [Order article via Infotrieve]
  • Cole, M.F.; Bowden, G.H.; Korts, D.C.; and Bowen, W.H. (1978): The Effect of Pyridoxine, Phytate and Invert Sugar on Production of Plaque Acids in situ in the Monkey (M. fascicularis), Caries Res 12:190-201.[Medline] [Order article via Infotrieve]
  • Creanor, S.L.; MacFarlane, T.W.; Mackenzie, D.; Weetman, D.A.; Strang, R.; and Stephen, K.W. (1986): Microbiology and Acid/Anion Profiles of Enamel Surface Plaque from an in situ Caries Appliance, Caries Res 20:392-397.[Medline] [Order article via Infotrieve]
  • Curtis, M. and Eastoe, J. (1978a): A Preliminary Investigation into the Identity of a Major Ninhydrin-positive Component of Dental Plaque Fluid from the Monkey Macaca fascicularis, Arch Oral Biol 23:421-423.[CrossRef][Medline] [Order article via Infotrieve]
  • Curtis, M. and Eastoe, J. (1978b): Comparison of Free Amino Acid Pools in Dental Plaque Fluid from Monkeys (Macaca fascicularis) fed on High and Low Sugar Diets, Arch Oral Biol 23:989-992.[CrossRef][Medline] [Order article via Infotrieve]
  • Curtis, M.A. and Kemp, C.W. (1984): Nitrogen Metabolism in Dental Plaque. In: Cariology Today, B. Guggenheim, Ed., Basel: Karger, pp. 212-222.
  • Dawes, C. and Dibdin, G.H. (1986): A Theoretical Analysis of the Effects of Plaque Thickness and Initial Salivary Sucrose Concentration on Diffusion of Sucrose into Dental Plaque and its Conversion to Acid during Salivary Clearance, J Dent Res 65:89-94.[Abstract/Free Full Text]
  • Distler, W. and Kroncke, A. (1983): The Acid Pattern in Human Dental Plaque, J Dent Res 62:87-91.[Abstract/Free Full Text]
  • Dodds, M.W.J. and Edgar, W.M. (1986): Effects of Dietary Sucrose Levels on pH Fall and Acid-anion Profile in Human Dental Plaque after a Starch Mouth-rinse, Arch Oral Biol 31:509-512.[CrossRef][Medline] [Order article via Infotrieve]
  • Edgar, W.M. (1981): Isophoretic Analysis of Major Acids in Human Plaque Fluid Before and After Sucrose Consumption, Zahn Mund Kieferheilkd 69:744 (Abst).
  • Edgar, W.M.; Bowen, W.H.; and Cole, M.F. (1981a): Development of Rampant Dental Caries, and Composition of Plaque Fluid and Saliva in Irradiated Primates, J Oral Pathol 10:284-295.[CrossRef][Medline] [Order article via Infotrieve]
  • Edgar, W.M.; Bowen, W.H.; and Cole, M.F. (1982): Protein Components in Saliva and Plaque Fluid from Irradiated Primates, J Oral Pathol 11:252-259.[CrossRef][Medline] [Order article via Infotrieve]
  • Edgar, W.M.; Cockburn, M.A.; and Jenkins, G.N. (1981b): Uptake of Fluoride and its Inhibitory Effects in Oral Microorganisms in Culture, Arch Oral Biol 26:615-623.[CrossRef][Medline] [Order article via Infotrieve]
  • Edgar, W.M.; Dodds, M.W.J.; and Higham, S.M. (1986a): Changes in Carboxylic Acid and Free Amino Acid Profiles in Human Dental.Plaque after a Carbohydrate Challenge in situ, Biochem Soc Trans 14:977.
  • Edgar, W.M.; Dodds, M.W.J.; and Higham, S.M. (1986b): The Control of Plaque pH and its Significance in Relation to the Evaluation of Food Cariogenicity. In: Factors Relating to Demineralisation and Remineralisation of the Teeth, S.A. Leach, Ed., Oxford: IRL Press, pp. 115-126.
  • Edgar, W.M. and Tatevossian, A. (1969): The Aqueous Phase of Plaque. In: Tooth Enamel II, M.V. Stack and R. Fearnhead, Eds., Bristol: John Wright, pp. 229-232.
  • Geddes, D.A.M. (1975): Acids Produced by Human Dental Plaque Metabolism in situ, Caries Res 9:98-109.[Medline] [Order article via Infotrieve]
  • Geddes, D.A.M. (1984): Current View of Plaque Acidogenicity. In: Cariology Today, B. Guggenheim, Ed., Basel: Karger, pp. 199-204.
  • Gilmour, M.N.; Greene, G.C.; Zahn, L.M.; Sparmann, C.D.; and Pearlman, J. (1976): C1-C4 Monocarboxylic and Lactic Acids in Dental Plaques Before and After Exposure to Sucrose in vivo. In: Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Sp. Supp. MicrobiolAbstr, Washington, DC: Information Retrieval, Inc., pp. 491-520.
  • Higham, S.M. and Edgar, W.M. (1988): Lactate Dehydrogenase Activity in Human Dental Plaque and its Effect on Metabolism, J Dent Res 67:675, Abst. No. 288.
  • Higham, S.M. and Edgar, W.M. (1989a): Human Dental Plaque pH, and the Organic Acid and Free Amino Acid Profiles in Plaque Fluid, After Sucrose Rinsing, Arch Oral Biol 34:329-334.
  • Higham, S.M. and Edgar, W.M. (1989b): Effects of Parafilm and Cheese Chewing on Human Dental Plaque pH and Metabolism, Caries Res 23:42-48.[Medline] [Order article via Infotrieve]
  • Hsu, S.D. and Cole, M.F. (1983): Integrity of Host Defense Factors in Dental Plaque Fluid, J Dent Res 62:279, Abst. No. 989.
  • Jenkins, G.N. and Edgar, W.M. (1977): The Distribution and Forms of F in Saliva and Plaque, Caries Res 11 (Suppl. 1):226-242.[Medline] [Order article via Infotrieve]
  • Jenkins, G.N.; Edgar, W.M.; and Ferguson, D.B. (1969): The Distribution and Metabolic Effects of Human Plaque Fluorine, Arch Oral Biol 14:105-119.[CrossRef][Medline] [Order article via Infotrieve]
  • Keevil, C.W.; McDermid, A.S.; Marsh, P.D.; and Ell-Wood, D.C. (1986): Protonmotive Force Driven 6-Deoxyglucose Uptake by the Oral Pathogen Streptococcus mutans Ingbritt, Arch Microbiol 146:118-124.[CrossRef][Medline] [Order article via Infotrieve]
  • Keevil, C.W.; West, A.A.; Bourne, N.; and Marsh, P.D. (1984): Inhibition of the Synthesis and Secretion of Extracellular Glucosyl- and Fructosyltransferase in Streptococcus sanguis by Sodium Ions, J Gen Microbiol 130:77-82.[Abstract/Free Full Text]
  • Margolis, H.C.; Duckworth, J.H.; and Moreno, E.C. (1988a): Composition of Pooled Resting Plaque Fluid from Caries-free and Caries-susceptible Individuals, J Dent Res 67:1468-1475.[Abstract/Free Full Text]
  • Margolis, H.C.; Duckworth, J.H.; and Moreno, E.C. (1988b): Composition and Buffer Capacity of Pooled Starved Plaque Fluid from Caries-free and Caries-susceptible Individuals, J Dent Res 67:1476-1482.[Abstract/Free Full Text]
  • Moreno, E.C. and Margolis, H.C. (1988): Composition of Human Plaque Fluid, J Dent Res 67:1181-1189.[Abstract/Free Full Text]
  • Newman, H.N. (1979): The Approximal Apical Border of Plaque on Children's Teeth. 2. Adhesion, Interbacterial Connections and Carbohydrate Metabolism, J Periodontol 50:568-576.
  • Singer, D.L. and Kleinberg, I. (1983): The Free Amino Acids in Human Dental Plaque, Arch Oral Biol 28:873-878.[CrossRef][Medline] [Order article via Infotrieve]
  • Tatevossian, A. and Gould, C.T. (1976): The Composition of the Aqueous Phase in Human Dental Plaque, Arch Oral Biol 21:319-326.[CrossRef][Medline] [Order article via Infotrieve]
  • Tatevossian, A. and Newbrun, E. (1979): Enzymic Activities in the Aqueous Phase of Human Dental Plaque, Arch Oral Biol 24:657-662.[CrossRef][Medline] [Order article via Infotrieve]
  • Tatevossian, A. and Newbrun, E. (1981): Electrophoretic and Immunoelectrophoretic Studies of Proteins in the Aqueous Phase of Human Dental Plaque, Arch Oral Biol 26:275-280.[CrossRef][Medline] [Order article via Infotrieve]
  • Vratsanos, S.M. and Mandel, I.D. (1982): A Comparative Plaque Acidogenesis of Caries-resistant vs. Caries-susceptible Adults, J Dent Res 61:465-468.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 69, No. 6, 1332-1336 (1990)
DOI: 10.1177/00220345900690062101
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This article has been cited by other articles:


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