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The Possible Role of Oral Epithelial Cells in Tissue-type Plasminogen Activator-related Fibrinolysis in Human Saliva

Department of Oral and Maxillofacial Surgery, Århus University Hospital, Nørrebrogade, DK-8000 Århus C, Denmark

J. Gram

Section of Coagulation and Fibrinolysis, Department of Clinical Chemistry, Ribe County Hospital, Østergade, DK-6700 Esbjerg, Denmark

J. Jespersen

Section of Coagulation and Fibrinolysis, Department of Clinical Chemistry, Ribe County Hospital, Østergade, DK-6700 Esbjerg, Denmark

We studied the fibrinolytic activities of the following subfractions of unstimulated human whole saliva on plasminogen-rich fibrin plates: (1) submandibular saliva, (2) parotid saliva, and (3) smears of buccal epithelial cells from ten healthy males. A cell-bound plasminogen activator could be demonstrated in the sediments of all three subfractions of whole saliva. The incorporation of antibodies (goat IgG) against human two-chain tissue-type plasminogen activator (t-PA) could quench the assessed fibrinolytic activities, whereas additional experiments suggested the absence of urokinase-like and F XII-dependent activators of fibrinolysis. The determinations in growth medium from buccal-epithelial cell culture of t-PA antigen by means of enzyme-linked immunosorbent assay showed the presence of t-PA.

These clinical and experimental findings suggest that buccal-epithelial cells produce t-PA, while the activity of t-PA in parotid and submandibular saliva is very low.

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Journal of Dental Research, Vol. 69, No. 6, 1283-1286 (1990)
DOI: 10.1177/00220345900690061201


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Sindet-Pedersen, S. Articles by Jespersen, J. Search for Related Content PubMed PubMed Citation Articles by Sindet-Pedersen, S. Articles by Jespersen, J. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?