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Effect of pH on the Growth and Proteolytic Activity of Porphyromonas gingivalis and Bacteroides intermedius
N. Takahashi
Department of Oral Sciences and Dental Research Institute, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455
C.F. Schachtele
Department of Oral Sciences and Dental Research Institute, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455
The effect of pH on the growth and proteolytic activity of the type strain and fresh isolates of Porphyromonas gingivalis and Bacteroides intermedius was investigated. B. intermedius strains grew with and without glucose at a pH as low as 5.0. These bacteria grew almost as well as Streptococcus mutans at pH 5.0 and better than Actinomyces viscosus at pH 5.5 and 5.0. Some B. intermedius strains raised the culture pH when grown at a low pH without glucose. In contrast, P. gingivalis strains grew only at pH 6.5 to 7.0. The P. gingivalis strains had proteolytic activities against azocoll, azocasein, and azoalbumin, while the B. intermedius strains degraded azocasein and azoalbumin, but not azocoll. B. intermedius showed maximum proteolytic activity at pH 7.0, and high activity over a wide pH range. In contrast, the optimum pH of proteolytic activity in P. gingivalis was pH 7.5 to 8.0. The P. gingivalis activities were more sensitive than those of B. intermedius to low pH. The capacity of B. intermedius to degrade proteins to more readily metabolizable substrates at low pH might explain the growth of this bacterium in an acidic environment. These differences between B. intermedius and P. gingivalis could explain their capacity to survive at different sites in the oral cavity and indicate how B. intermedius might positively influence the growth of P. gingivalis in subgingival plaque.
REFERENCES
- Gibson, S.A.W. and MacFarlane, G.T. (1988): Studies on the Proteolytic Activity of Bacteroides fragilis, J Gen Microbiol 134:19-27.[Abstract/Free Full Text]
- Hamilton, I.R.; McKee, A.S.; and Bowden, G.H. (1989): Growth and Metabolic Properties of Bacteroides intermedius in Anaerobic Continuous Culture, Oral Microbiol Immunol 4:89-97.[Medline]
[Order article via Infotrieve]
- Harper, D.S. and Loesche, W.J. (1984): Growth and Acid Tolerance of Human Dental Plaque Bacteria, Arch Oral Biol 29:842-848.
- Holdeman, L.V.; Kelley, R.W.; and Moore, W.E.C. (1984): Family I Bacteroidaceae, Pribram 1933. In: Bergey's Manual of Determinative Bacteriology, 9th ed., N.R. Krieg and J.C. Holt, Eds., Baltimore: Williams and Wilkins, pp. 602-662.
- Johnson, J.L. and Holdeman, L.V. (1983): Bacteroides intermedius comb. nov. and Descriptions of Bacteroides corporis sp. nov., Int J Syst Bacteriol 33:15-25.[Abstract/Free Full Text]
- Kiel, R.A.; Kornman, K.S.; and Robertson, P.B. (1983): Clinical and Microbiological Effects of Localized Ligature-induced Periodontitis on Non-ligated Sites in the Cynomolgus Monkey, J Periodont Res 18:200-211.[CrossRef][Medline]
[Order article via Infotrieve]
- Kornman, K.S.; Holt, S.C.; and Robertson, P.B. (1981): The Microbiology of Ligature-induced Periodontitis in the Cynomolgus Monkey, J Periodont Res 16:363-371.[CrossRef][Medline]
[Order article via Infotrieve]
- Loesche, W.J.; Syed, S.A.; Schmidt, E.; and Morrison, E.C. (1985): Bacterial Profiles of Subgingival Plaques in Periodontitis, J Periodontol 56:447-456.[Medline]
[Order article via Infotrieve]
- Mayrand, D. and Grenier, D. (1985): Detection of Collagenase Activity in Oral Bacteria, Can J Microbiol 31:134-138.[Medline]
[Order article via Infotrieve]
- Mayrand, D. and McBride, B.C. (1980): Ecological Relationships of Bacteria Involved in a Simple, Mixed Anaerobic Infection, Infect Immun 27:44-50.[Abstract/Free Full Text]
- McDermid, A.S.; McKee, A.S.; and Marsh, P.D. (1988): Effect of Environmental pH on Enzyme Activity and Growth of Bacteroides gingivalis W50, Infect Immun 56:1096-1100.[Abstract/Free Full Text]
- Millet, J. (1970): Characterization of Proteinases Excreted by Bacillus subtilis Marburg Strain During Sporulation, J Appl Bacteriol 33:207-219.[Medline]
[Order article via Infotrieve]
- Moore, L.V.H.; Moore, W.E.C.; Cato, E.P.; Smibert, R.M.; Burmeister, J.A.; Best, A.M.; and Ranney, R.R. (1987): Bacteriology of Human Gingivitis, J Dent Res 66:989-995.[Abstract/Free Full Text]
- Moore, W.E.C.; Holdeman, L.V.; Cato, E.P.; Smibert, R.M.; Burmeister, J.A.; and Ranney, R.R. (1983): Bacteriology of Moderate (Chronic) Periodontitis in Mature Adult Humans, Infect Immun 42:510-515.[Abstract/Free Full Text]
- Moore, W.E.C.; Holdeman, L.V.; Cato, E.P.; Smibert, R.M.; Burmeister, J.A.; Palcanis, K.C.; and Ranney, R.R. (1985): Comparative Bacteriology of Juvenile Periodontitis, Infect Immun 48:507-519.[Abstract/Free Full Text]
- Rudek, W. and Haque, R.-U. (1976): Extracellular Enzymes of the Genus Bacteroides, J Clin Microbiol 4:455-60.[Abstract/Free Full Text]
- Schachtele, C.F. and Jensen, M.E. (1982): Comparison of Methods for Monitoring Changes in the pH of Human Dental Plaque, J Dent Res 61:1117-1125.[Abstract/Free Full Text]
- Shah, H.N. and Collins, M.D. (1988): Proposal of Reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a New Genus, Porphyromonas, Int J Syst Bacteriol 38:128-131.[Abstract/Free Full Text]
- Shah, H.N. and Williams, R.A.D. (1987): Utilization of Glucose and Amino Acids by Bacteroides intermedius and Bacteroides gingivalis, Curr Microbiol 15:241-246.[CrossRef]
- Shah, H.N.; Williams, R.A.D.; Bowden, G.H.; and Hardie, J.M. (1976): Comparison of the Biochemical Properties of Bacteroides melaninogenicus from Human Dental Plaque and Other Sites, J Appl Bacteriol 41:473-492.[Medline]
[Order article via Infotrieve]
- Slots, J. (1977): The Predominant Cultivable Microflora of Advanced Periodontitis, Scand J Dent Res 85:114-121.[Medline]
[Order article via Infotrieve]
- Slots, J. (1982): Importance of Black-pigmented Bacteroides in Human Periodontal Disease. In: Host-parasite Interactions in Periodontal Diseases, R.J. Genco and S.E. Mergenhagen, Eds., Washington D.C.: American Society for Microbiology, pp. 27-45.
- Slots, J.; Moenbo, D.; Langebaek, J.; and Frandsen, A. (1978): Microbiota of Gingivitis in Man, Scand J Dent Res 86:174-181.[Medline]
[Order article via Infotrieve]
- Socransky, S.S.; Dzink, J.L.; and Smith, C.M. (1985): Chemically Defined Medium for Oral Microorganisms, J Clin Microbiol 22:303-305.[Abstract/Free Full Text]
- Socransky, S.S.; Haffajee, A.D.; Dzink, J.L.; and Hillman, J.D. (1988): Associations Between Microbial Species in Subgingival Plaque Samples, Oral Microbiol Immunol 3:1-7.[Medline]
[Order article via Infotrieve]
- Spiegel, C.A.; Hayduk, S.E.; Minah, G.E.; and Krywolap, G.N. (1979): Black-Pigmented Bacteroides from Clinically Characterized Periodontal Sites, J Periodont Res 14:376-382.[CrossRef][Medline]
[Order article via Infotrieve]
- Sundqvist, G.; Carlsson, J.; and Hanstrom, L. (1987): Collagenolytic Activity of Black-Pigmented Bacteroides Species, J Periodont Res 22:300-306.[CrossRef][Medline]
[Order article via Infotrieve]
- Tanner, A.C.R.; Haffer, C.; Bratthall, G.T.; Visconti, R.A.; and Socransky, S.S. (1979): A Study of the Bacteria Associated with Advancing Periodontitis in Man, J Clin Periodontol 6:278-307.[CrossRef][Medline]
[Order article via Infotrieve]
- Wahren, A. and Gibbons, R.J. (1970): Amino Acid Fermentation by Bacteroides melaninogenicus, Antonie van Leeuwenhoek 36:149-159.[CrossRef][Medline]
[Order article via Infotrieve]
- White, D. and Mayrand, D. (1981): Association of Oral Bacteroides with Gingivitis and Adult Periodontitis, J Periodont Res 16:259-265.[CrossRef][Medline]
[Order article via Infotrieve]
- Wijeyeweera, R.L. and Kleinberg, I. (1989): Arginolytic and Ureolytic Activities of Pure Cultures of Human Oral Bacteria and Their Effects on the pH Response of Salivary Sediment and Dental Plaque in vitro, Arch Oral Biol 34:43-53.[CrossRef][Medline]
[Order article via Infotrieve]
- Zambon, J.J.; Reynolds, H.S.; and Slots, J. (1981): Black-pigmented Bacteroides sp. in the Human Oral Cavity, Infect Immun 32:198-203.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 69, No. 6,
1266-1269 (1990)
DOI: 10.1177/00220345900690060801
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