Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Stookey, G.K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Stookey, G.K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Evaluation of Mutagenicity of Restorative Dental Materials Using the Ames Salmonella/Microsome Test

Y. Li

Indiana University School of Dentistry, 415 Lansing Street, Indianapolis, Indiana 46202

T.W. Noblitt

Indiana University School of Dentistry, 415 Lansing Street, Indianapolis, Indiana 46202

A.J. Dunipace

Indiana University School of Dentistry, 415 Lansing Street, Indianapolis, Indiana 46202

G.K. Stookey

Indiana University School of Dentistry, 415 Lansing Street, Indianapolis, Indiana 46202

Compounds of five commercially available dental material kits were examined for mutagenic potential by use of the Ames Salmonella/microsome test. Dimethyl sulfoxide (DMSO) was used as the solvent for all materials, except MONO-LOK2 Primer and Right-On Activator, which were dissolved in 95% ethanol. The Tenure, Fuji, and Gluma materials were initially screened at 2 mg (solids) or 2 µL (liquids) per plate, without S9 (Aroclor 1254-induced rat liver microsomes), and dose-response studies were conducted for those materials showing potential mutagenicity. The components of three dental kits, MONO-LOK2, Right-On, and Gluma, were further examined, both with and without S9 activation, at doses of 0.2, 4, 20, 100, 500, and 2500 µg (nL for liquid components) per plate. The data showed no mutagenic potential for the components of Fuji Glass Ionomer (Type III), Tenure Dentin Bonding System, and MONO-LOK2 kits, or Right-On Paste. However, Gluma 3, a component of the Gluma/Lumifor Bonding System, exhibited mutagenic activity in a dose-response manner. The mutagenicity of Gluma 3 was demonstrated in repeated experiments. Gluma 4 and the resin of the Gluma dental kit, as well as Right-On Activator, caused a slight increase in the number of revertants, and judgment regarding their mutagenicity could not be made conclusively. The results of this study indicate that some commercial dental materials may not have been adequately tested for mutagenicity, and that a reliable test program for evaluation of mutagenicity of dental materials is needed so that their safe use in dental practice can be ensured.

REFERENCES

  • American Dental Association, Council On Dental Materials And Devices (1972): Recommended Standard Practices for Biological Evaluation of Dental Materials, JAm Dent Assoc 84:382-387.
  • American Dental Association, Council On Dental Materials, Instruments, And Equipment (1982): Addendum to American National Standards Institute/American Dental Association Document No. 41 for Recommended Standard Practices for Biological Evaluation of Dental Materials, JAm Dent Assoc 104:680.
  • American National Standards Institute/American Dental Association (1979): Document No. 41 for Recommended Standard Practices for Biological Evaluation of Dental Materials, J Am Dent Assoc 99:697-698.[Medline] [Order article via Infotrieve]
  • American National Standards Institute (1989 ): Final Actions on American National Standards: Dental Materials, ANSI Report 23:8, March 17.
  • Ames, B.N.: McCann, J.; and Yamasaki, E. (1975): Methods for Detecting Carcinogens and Mutagens with the Salmonella/ Mammalian-Microsome Mutagenicity Test, Mutat Res 34:317-364.
  • Ames, B.N. and Yanofsky, C. (1971): The Detection of Chemical Mutagens with Enteric Bacteria. In: Chemical Mutagens, Principles and Methods of Their Detection, Vol. 1, A. Hollaender, Ed., New York: Plenum Press, pp. 267-287.
  • Anderson, D.A.F.; Ferracane, J.L.; Zimmermann, E.R.; and Kaga, M. (1988): Cytotoxicity of Variably Cured Light-activated Dental Composites, J Dent Res 67:226, Abst. No. 905.
  • Asmussen, E. and Bowen, R.L. (1987): Effect of Acidic Pretreatment on Adhesion to Dentin Mediated by Gluma, J Dent Res 6:1386-1388.
  • Athas, W.F.; Gutzke, G.E.; Kubinski, Z.O.; and Kubinski, H. (1979): In vitro Studies on the Carcinogenic Potential of Orthodontic Bonding Materials, Ecotoxicol Environ Safety 3:401-410.[CrossRef][Medline] [Order article via Infotrieve]
  • Craig, R.G. and Hanks, C.T. (1985): Cytotoxicity of Experimental Casting Alloys by Cell Culture Testing, J Dent Res 68:322, Abst. No. 1121.
  • Cross, N.G.; Taylor, R.F.; and Nunez, L.J. (1983): "Single-Step" Orthodontic Bonding Systems: Possible Mutagenic Potential, Am J Orthod 84:344-350.[CrossRef][Medline] [Order article via Infotrieve]
  • De Serres, F.J. and Matsushima, T. (1987): Meeting Report: Deployment of Short-Term Assays for Environmental Mutagens and Carcinogens, Mutat Res 182:173-184.[Medline] [Order article via Infotrieve]
  • Federation Dentaire Internationale, Commission On Dental Materials, Instruments, Equipment And Therapeutics (1980): Recommended Standard Practices for Biological Evaluation of Dental Materials, Int Dent J 30:140-188.[Medline] [Order article via Infotrieve]
  • Fredericks, H.E. (1981): Mutagenicity Potential of Orthodontic Bonding Materials, Am J Orthod 80:316-324.[CrossRef][Medline] [Order article via Infotrieve]
  • International Organization For Standardization ( 1984): Biological Evaluation of Dental Materials, Technical Report 7405, Geneva: ISO.
  • Kaga, M.; Seals, N.S.; Hanawa, T.; Ferracane, J.L.; and Okabe, T. (1987): Cytotoxicity of Amalgams, J Dent Res 6:134, Abst. No. 217.
  • Lemons, J.E.; Lucas, L.C.; Henson, P.; and Hill, C. (1988): Biocompatibility of Copper Base Alloys, J Dent Res 67:262, Abst. No. 1196.
  • Levin, D.E.; Hollstein, M.; Christman, M.F.; Schwiers, E.A.; and Ames, B.N. (1982): A New Salmonella Tester Strain (TA102) with A-T Base Pairs at the Site of Mutation Detects Oxidative Mutagens, Proc Natl Acad Sci USA 79:7445-7449.[Abstract/Free Full Text]
  • Lewis, B.B. and Chestner, S.B. (1981): Formaldehyde in Dentistry: A Review of Mutagenic and Carcinogenic Potential, JAm Dent Assoc 103:429-434.
  • Li, Y.; Noblitt, T.W.; Dunipace, A.J.; and Stookey, G.K. (1989): Mutagenicity Studies of Dental Materials Using the Ames Test, J Dent Res 68:322, Abst. No. 1226.
  • Marnett, L.J.; Hurd, H.K.; Hollstein, M.; Levin, D.E.; Esterbauer, H.; and Ames, B.N. (1985): Naturally Occurring Carbonyl Compounds are Mutagens in Salmonella Tester Strain TA104, Mutat Res 148:25-34.[Medline] [Order article via Infotrieve]
  • Maron, D.M. and Ames, B.N. (1983): Revised Methods for the Salmonella Mutagenicity Test, Mutat Res 113:173-215.[CrossRef][Medline] [Order article via Infotrieve]
  • Miller, E.G.; Washington, V.H.; Bowles, W.H.; and Zimmermann, E.R. (1986): Mutagenic Potential of Some Chemical Components of Dental Materials, Dent Mater 2:163-165.[CrossRef][Medline] [Order article via Infotrieve]
  • National Institute Of Dental Research (1984 ): Workshop: Biocompatibility of Metals in Dentistry, J Am Dent Assoc 109:469-471.[Medline] [Order article via Infotrieve]
  • Orstavik, D. and Hongslo, J.K. (1985): Mutagenicity of Endodontic Sealers, Biomaterials 6:129-132.[CrossRef][Medline] [Order article via Infotrieve]
  • Phillips, R.W. (1988): Changing Trends in Dental Biomaterials Science, Int J Oral Maxillofac Imp 3:79-80.
  • Ruiz-Rubio, M.; Alejander-Duran, E.; and Pueyo, C. (1985): Oxidative Mutagens Specific for A-T Base Pairs Induce Forward Mutations to L-arabinose Resistance in Salmonella typhimurium, Mutat Res 147:153-163.[CrossRef][Medline] [Order article via Infotrieve]
  • Sokal, R.R. and Rohlf, F.J. (1982): Biometry, the Principle and Practice of Statistics in Biological Research, 2nd ed., San Francisco: W.H. Freeman and Co.
  • Snodgrass, J.G. (1977): The Number Game, Statistics for Psychology, Baltimore: The Williams and Wilkins Co.
  • Stanford, J.W. (1989): Control of Safety and Efficacy ADA Specifications Program, J Dent Res 68:174, Abst. No. S2.
  • Woolverton, C.T.; Fotos, P.; Mokas, M.; and Mermi-Gas, M. (1986): Evaluation of Eugenol for Mutagenicity by the Mouse Micronucleus Test, J Oral Pathol 15:450-453.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 5, 1188-1192 (1990)
DOI: 10.1177/00220345900690051301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
W. Geurtsen
Biocompatibility of Resin-Modified Filling Materials
Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(3): 333 - 355.
[Abstract] [Full Text] [PDF]

Home page
 ADRHome page
S. C. Bayne
Dental Composites/Glass Ionomers: Clinical Reports
Advances in Dental Research, September 1, 1992; 6(1): 65 - 77.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Stookey, G.K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Stookey, G.K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?