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The Crown Odontoblasts of Rat Molars from Primary Dentinogenesis to Complete Eruption

Department of Human Anatomy and Histology, Section "E. Allara" - Viale Pieraccini 6, 50139 Firenze, Italy

G. Mancini

Department of Human Anatomy and Histology, Section "E. Allara" - Viale Pieraccini 6, 50139 Firenze, Italy

F. Galeotti

Department of Human Anatomy and Histology, Section "E. Allara" - Viale Pieraccini 6, 50139 Firenze, Italy

E. Francini

Department of Human Anatomy and Histology, Section "E. Allara" - Viale Pieraccini 6, 50139 Firenze, Italy

P. Pierleoni

Institute of Odontognathostomatology of the University of Florence - Viale Morgagni 85, 50134 Firenze, Italy

The involution of crown odontoblasts after primary dentinogenesis in teeth of limited eruption is discussed. The odontoblasts of rat first lower molars were analyzed morphometrically from the tenth day to the 40th day of age, i.e., from the late phase of primary dentinogenesis to complete eruption. All the organelles underwent atrophy, but at different rates. In particular, the membranes of the endoplasmic reticulum decreased progressively in surface area from day 10 to day 40, whereas those of the Golgi apparatus decreased significantly between day 10 and day 14, and then remained practically unchanged in size. The volume of the lysosome compartment never increased beyond that during primary dentinogenesis. The profile length of the endoplasmic reticulum in each observed cell section was taken as an estimate of secretory activity. At day 40, this organelle was smaller in ~95% of the cells than it had been in any cell at day 10. These results suggest that cell atrophy may occur without any increase in the degradation processes of the cytoplasmic components and that the organelles along the secretory pathway may have independent regulatory systems. In the odontoblasts, as in several types of secretory epithelial cells, only a small fraction of the cells is engaged in appreciable secretory activity. This occurs, however, when the overall activity of the same cell population is relatively low.

REFERENCES

• Bacallao, R.; Antony, C.; Dotti, C.; Karsenti, E.; Stelzer, E.H.K.; and Simons, K. (1989): The Subcellular Organization of Madin-Darby Canine Kidney Cells During the Formation of a Polarized Epithelium, J Cell Biol 109:2817-2832.[Abstract/Free Full Text]
• Baddeley, A.J.; Gundersen, H.J.G.; and Cruz Orive, L.M. (1986): Estimation of Surface Area from Vertical Sections, J Microsc 142:259-276.[Medline] [Order article via Infotrieve]
• Bronckers, A.L.J.J.; Lyaruu, D.M.; Bervoets, T.J.M.; and Woltgens, J.H.M. (1988): The Effect of Colchicine on Protein Secretion by Differentiating Odontoblasts and Ameloblasts in the Hamster Tooth in vitro as Shown by Radioautography with 3H-Proline, Cell Tissue Res 252:631-638.[CrossRef][Medline] [Order article via Infotrieve]
• Couve, E. (1986): Ultrastructural Changes During the Life Cycle of Human Odontoblasts, Arch Oral Biol 31:643-651.[CrossRef][Medline] [Order article via Infotrieve]
• Francioni, E.; Defraia, E.; Pierleoni, P.; and Romagnoli, P. (1987): The Odontoblasts of Human, Fully Erupted Teeth, Acta Anat 130:33, Abst. No. 135.
• Galeotti, F.; Fallai, S.; Pagavino, G.; Pierleoni, P.; and Romagnoli, P. (1990): The Intercellular Substance of the Pulp of Human, Fully Erupted, Permanent Premolar Teeth, Z Mikr-Anat Forsch, in press.
• Garant, P.R. and Cho, M.T. (1985): Ultrastructure of the Odontoblast. In: The Chemistr md Biology of Mineralized Tissues, W.T. Butler, Ed., Birmingham, AL: EBSCO Media, pp. 21-32.
• Garant, P.R.; Szabo, G.; and Nalbandian, J. (1968): The Fine Structure of the Mouse Odontoblast, Arch Oral Biol 13:857-876.[CrossRef][Medline] [Order article via Infotrieve]
• Gartner, L.P.; Seibel, W.; Hiatt, J.L.; and Provenza, D.V. (1979): A Fine Structural Analysis of Mouse Molar Odontoblast Maturation, Acta Anat 103:16-33.[Medline] [Order article via Infotrieve]
• Herzog, V.; Fuchs, S.; and Romagnoli, P. (1988): Transcytosis in Thyroid Follicle Cells is Selectively Stimulated by TSH, Eur J Cell Biol 47 (Suppl. 23):26, Abst. No. 69.
• Holland, G.R. (1985): The Odontoblast Process: Form and Function, J Dent Res 64:499-514.[Medline] [Order article via Infotrieve]
• Karnovsky, M.J. (1967): The Ultrastructural Basis of Capillary Permeability Studied with Peroxidase as a Tracer, J Cell Biol 35:213-236.[Abstract/Free Full Text]
• Larsson, A. and Bloom, G. (1973): Studies on Dentinogenesis in the Rat. Fine Structure of Developing Odontoblasts and Pre-dentine in Relation to the Mineralization Process, Z Anat Entwickl-Gesch 139:227-246.[CrossRef][Medline] [Order article via Infotrieve]
• Leblond, C.P. and Wright, G.M. (1981): Steps in the Elaboration of Collagen by Odontoblasts and Osteoclasts, Meth Cell Biol 23:167-189.[CrossRef][Medline] [Order article via Infotrieve]
• Lentner, C.; Lentner, C.; and Wink, A. (1982): Geigy Scientific Tables, Vol. 2, 8th ed., Basel: Ciba-Geigy, pp. 201-210.
• Nogueira, T.O.; Zorn, T.M.T.; Stene, T.; and Koppary, H.S. (1988): Autoradiographic Investigation of Effects of High-dose Colchicine on Dentinogenesis in Rat Incisors, Acta Anat 131:45-49.
• Rodriguez-Boulan, E. and Salas, P.J.I. (1989): External and Internal Signals for Epithelial Cell Surface Polarization, Ann Rev Physiol 51:741-754.[CrossRef][Medline] [Order article via Infotrieve]
• Romagnoli, P. (1984): The Golgi Apparatus and Lysosomes of Rat Pancreatic Acinar Cells Following Refeeding, Histochem J 16:855-868.[CrossRef][Medline] [Order article via Infotrieve]
• Romagnoli, P. (1988): Increases in Apical Plasma Membrane Surface Paralleling Enzyme Secretion from Exocrine Pancreatic Acinar Cells, Pancreas 3:189-192.[Medline] [Order article via Infotrieve]
• Ruch, J.V.; Karcher-Djuricic, V.; Staubli, A.; and Fabre, M. (1975): Effects de la Cytocalasine B et de la Colchicine sur les Cytodifférenciations Dentaires in vitro, Arch Anat Microsc Morph Exp 64:113-134.[Medline] [Order article via Infotrieve]
• Schour, I. and Massler, M. (1949): The Teeth. In: The Rat in Laboratory Investigation, E.J. Farris and J.Q. Griffith, Eds., New York: Hafner Publishing Co., pp. 104-165.
• Schuit, F.C.; In't Veld, P.A.; and Pipeleers, D.G. (1988): Glucose Stimulates Proinsulin Biosynthesis by a Dose-dependent Recruitment of Pancreatic Beta Cells, Proc Natl Acad Sci USA 85:3865-3869.[Abstract/Free Full Text]
• Spurr, A.R. (1969): A Low-viscosity Epoxy Resin Embedding Medium for Electron Microscopy, J Ultrastruct Res 26:31-43.[CrossRef][Medline] [Order article via Infotrieve]
• Takuma, S. and Nagai, N. (1971): Ultrastructure of Rat Odontoblasts in Various Stages of their Development and Maturation, Arch Oral Biol 16:993-1011.[CrossRef][Medline] [Order article via Infotrieve]
• Torneck, C.D. (1986): Dentin-pulp Complex. In: Oral Histology, Development, Structure and Function, A.R. Ten Cate, Ed., St. Louis: C.V. Mosby, pp. 140-166.
• Van Deurs, B.; Hansen, S.H.; Petersen, O.W.; Lokken Melby, E.; and Sandvig, K. (1990): Endocytosis, Intracellular Transport and Transcytosis of the Toxic Protein Ricin by a Polarized Epithelium, Eur J Cell Biol 51:96-109.[Medline] [Order article via Infotrieve]
• Warshawsky, H. and Josephsen, K. (1981): The Behaviour of Substances Labeled with 3H-Proline and 3H-Fucose in the Cellular Processes of Odontoblasts and Ameloblasts, Anat Rec 200:1-10.[CrossRef][Medline] [Order article via Infotrieve]
• Warshawsky, H. and Moore, G. (1967): A Technique for the Fixation and Decalcification of Rat Incisors for Electron Microscopy, J Histochem Cytochem 15:542-549.[Abstract]
• Weibel, E.R. (1979): Stereological Methods, Vol. 1, London: Academic Press, pp. 116-134.
• Weinstock, M. and Leblond, C.P. (1974): Synthesis, Migration, and Release of Precursor Collagen by Odontoblasts as Visualised by Radioautography after [3H]Proline Administration, J Cell Biol 60:92-127.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 69, No. 12, 1857-1862 (1990)
DOI: 10.1177/00220345900690121301


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