Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Hormia, M.
Right arrow Articles by Virtanen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hormia, M.
Right arrow Articles by Virtanen, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Expression of Integrins in Human Gingiva

M. Hormia

Department of Periodontology, University of Helsinki, Mannerheimintie 172, SF-00300 Helsinki, Finland

J. Ylänne

Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, SF-00170 Helsinki, Finland

I. Virtanen

Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, SF-00170 Helsinki, Finland

The distribution of the {alpha} 1-{alpha}6 subunits of β1 integrins was studied by using a panel of monoclonal antibodies in indirect immunofluorescence microscopy. The results showed that the β1 subunit was expressed at the cell membrane of basal cells of gingival epithelium, throughout the cells of junctional epithelium (JE), and in cells of connective tissue, including endothelial cells. The {alpha}1 subunit was expressed in vascular endothelial cells and, more faintly, in inflammatory cells in gingival connective tissue. The {alpha}4 subunit was expressed selectively in inflammatory cells, and the {alpha}5 subunit was expressed in cells throughout gingival connective tissue. An overall cell membrane immunoreactivity for the {alpha}2 and {alpha}3 subunits was shown in basal cells of gingival epithelium and in cells of JE, corresponding to the epithelial localization of the β1 subunit. The {alpha}6 subunit was polarized to the basal aspects of basal epithelial cells, but was also present in an overall cell surface distribution in basal cells and in cells of JE. The β4 integrin subunit was mainly expressed at the basal aspects of basal cells in gingival epithelium and JE. The results indicate that the {alpha}2/β1, {alpha}3/β 1, {alpha}6/β1, and {alpha}6/β 4 integrins are all expressed in human gingival epithelium. Of these, the {alpha}6/β4 integrin complex is the major candidate for mediation of the attachment of epithelial cells to the basement membrane facing the connective tissue and probably also the tooth.

REFERENCES

  • Akiyama, S.K.; Yamada, S.S.; Chen, W.-T.; and Yamada, K.M. (1989): Analysis of Fibronectin Receptor Function with Monoclonal Antibodies: Roles in Cell Adhesion, Migration, Matrix Assembly, and Cytoskeletal Organization, J Cell Biol 109:863-875.
  • Buck, C.A. and Horwitz, A.F. (1987): Cell Surface Receptors for Extracellular Matrix Molecules, Ann Rev Cell Biol 3:179-205.[CrossRef]
  • Carter, W.G.; Wayner, E.A.; Bouchard, T.S.; and Kaur, P. (1990): The Role of Integrins {alpha}2β1 and {alpha}3β1 in Cell-Cell and Cell-Substrate Adhesion of Human Epidermal Cells, J Cell Biol 110:1387-1404.[Abstract/Free Full Text]
  • Deporter, D.A. and Brown, D.Y. (1980): Fine Structural Observations on the Mechanism of Loss of Attachment During Experimental Periodontal Disease in the Rat, J Periodont Res 15:304-313.[CrossRef][Medline] [Order article via Infotrieve]
  • Ekblom, P.; Vestweber, D.; and Kemler, R. (1986): Cell-Matrix Interactions and Cell Adhesion During Development, Ann Rev Cell Biol 2:27-47.[CrossRef]
  • Gehlsen, K.R.; Dickerson, K.; Argraves, W.S.; Engvall, E.; and Ruoslahti, E. (1989): Subunit Structure of a Laminin-binding Integrin and Localization of its Binding Site on Laminin, J Biol Chem 264:19034-19038.[Abstract/Free Full Text]
  • Gehlsen, K.R.; Dillner, L.; Engvall, E.; and Ruoslahti, E. (1988): The Human Laminin Receptor is a Member of the Integrin Family of Cell Adhesion Receptors, Science 241:1228-1229.[Abstract/Free Full Text]
  • Giltay, J.C.; Brinkman, H.-J.M.; Modderman, P.W.; Von Dem Borne, A.E.; and Van Mourik, J.A. (1989): Human Vascular Endothelial Cells Express a Membrane Protein Complex Immunochemically Indistinguishable from the Platelet VLA-2 (Glycoprotein Ia-IIa) Complex, Blood 73:1235-1241.[Abstract/Free Full Text]
  • Hay, E.D. (1983): Cell and Extracellular Matrix: Their Organization and Mutual Dependence, Mod Cell Biol 2:509-548.
  • Hemler, M.E.; Crouse, C.; and Sonnenberg, A. (1989): Association of the VLA 6 Subunit with a Novel Protein. A Possible Alternative to the Common VLA β1 Subunit on Certain Cell Lines, J Biol Chem 264:6529-6535.[Abstract/Free Full Text]
  • Hemler, M.E.; Huang, C.; and Schwarz, L. (1987a): The VLA Protein Family. Characterization of Five Distinct Cell Surface Hetero-dimers Each with a Common 130,000 Molecular Weight β Subunit, J Biol Chem 262:3300-3309.[Abstract/Free Full Text]
  • Hemler, M.E.; Huang, C.; Takada, Y.; Schwarz, L.; Strominger, J.L.; and Clabby, M.L. (1987b): Characterization of the Cell Surface Heterodimer VLA-4 and Related Peptides, J Biol Chem 262:11478-11485.[Abstract/Free Full Text]
  • Hemler, M.E.; Sanchez-Madrid, F.; Flotte, T.J.; Krensky, A.M.; Burakoff, S.J.; Bhan, A.K.; Springer, T.A.; and Strominger, J.L. (1984): Glycoproteins of 210,000 and 130,000 M.W. on Activated T Cells: Cell Distribution and Antigenic Relation to Components on Resting Cells and T Cell Lines, J Immunol 132:3011-3018.[Abstract]
  • Hynes, R.O. (1986): Structure of Integrin, a Glycoprotein Involved in Transmembrane Linkage Between Fibronectin and Actin, Cell 46:271-282.[CrossRef][Medline] [Order article via Infotrieve]
  • Hynes, R.O. (1987): Integrins: A Family of Cell Surface Receptors, Cell 48:549-554.[CrossRef][Medline] [Order article via Infotrieve]
  • Ignotz, R.A. and Massague, J. (1987): Cell Adhesion Protein Receptors as Targets for Transforming Growth Factor-β Action, Cell 51:189-197.[CrossRef][Medline] [Order article via Infotrieve]
  • Kajiji, S.; Davceva, B.; and Quaranta, V. (1987): Six Monoclonal Antibodies to Human Pancreatic Cancer Antigens, Cancer Res 47:1367-1376.[Abstract/Free Full Text]
  • Kajiji, S.; Tamura, R.N.; and Quaranta, V. (1989): A Novel Integrin ({alpha}Eβ4) from Human Epithelial Cells Suggests a Fourth Family of Integrin Adhesion Receptors, EMBO J 8:673-680.[Medline] [Order article via Infotrieve]
  • Kramer, R.H. and Marks, N. (1989): Identification of Integrin Collagen Receptors on Human Melanoma Cells, J Biol Chem 264:4684-4688.[Abstract/Free Full Text]
  • Kunicki, T.J.; Nugent, D.J.; Staats, S.J.; Orchekowski, R.P.; Wayner, E.A.; and Carter, W.G. (1988): The Human Fibroblast Class II Extracellular Matrix Receptor Mediates Platelet Adhesion to Collagen and is Identical to the Platelet Glycoprotein la-IIa Complex, J Biol Chem 263:4516-4519.[Abstract/Free Full Text]
  • Laitinen, L.; Hormia, M.; and Virtanen, I. (1990): Psophocarpus tetragonolobus Agglutinin Reveals N-Acetyl Galactosaminyl Residues Confined to Endothelial Cells and Some Epithelial Cells in Human Tissues, J Histochem Cytochem 38:875-884.[Abstract]
  • Languino, L.R.; Gehlsen, K.R.; Wayner, E.; Carter, W.G.; Engvall, E.; and Ruoslahti, E. (1989): Endothelial Cells Use {alpha}2β1 Integrin as a Laminin Receptor, J Cell Biol 109:2455-2462.[Abstract/Free Full Text]
  • Larjava, H.; Peltonen, J.; Akiyama, S.K.; Yamada, S.S.; Gralnick, H.R.; Uitto, J.; and Yamada, K.M. (1990): Novel Function for β1 Integrins in Keratinocyte Cell-Cell Interactions, J Cell Biol 110:803-815.[Abstract/Free Full Text]
  • Liesi, P.; Dahl, D.; and Vaheri, A. (1983): Laminin is Produced by Early Rat Astrocytes in Primary Culture, J Cell Biol 96:920-924.[Abstract/Free Full Text]
  • Mackenzie, I.C. (1988): Factors Influencing the Stability of the Gingival Sulcus. In: Periodontology Today, B. Guggenheim, Ed., Basel: Karger AG, pp. 41-49.
  • Page, R.C. and Schroeder, H.E. (1976): Pathogenesis of Inflammatory Periodontal Disease. A Summary of Current Work, Lab Invest 34:235-249.[Medline] [Order article via Infotrieve]
  • Peltonen, J.; Larjava, H.; Jaakkola, S.; Gralnick, H.; Akiyama, S.K.; Yamada, S.S.; Yamada, K.M.; and Uitto, J. (1989): Localization of Integrin Receptors for Fibronectin, Collagen, and Laminin in Human Skin. Variable Expression in Basal and Squamous Cell Carcinomas, J Clin Invest 84:1916-1923.[Medline] [Order article via Infotrieve]
  • Pytela, R.; Piersbacher, M.D.; and Ruoslahti, E. (1985): Identification and Isolation of a 140 kD Surface Glycoprotein Expected of a Fibronectin Receptor, Cell 40:191-198.[CrossRef][Medline] [Order article via Infotrieve]
  • Ruoslahti, E. (1988): Fibronectin and its Receptor, Ann Rev Biochem 57:375-413.[CrossRef][Medline] [Order article via Infotrieve]
  • Ruoslahti, E. and Giancotti, F.G. (1989): Integrins and Tumor Cell Dissemination, Cancer Cells 1:119-126.[Medline] [Order article via Infotrieve]
  • Salonen, J. and Santti, R. (1985): Ultrastructural and Immunohistochemical Similarities in the Attachment of Human Oral Epithelium to the Tooth in vivo and to an Inert Substrate in an Explant Culture, J Periodont Res 20:176-184.[CrossRef][Medline] [Order article via Infotrieve]
  • Singer, I.I.; Scott, S.; Kawka, D.W.; Kazazis, D.M.; Gailit, J.; and Ruoslahti, E. (1988): Cell Surface Distribution of Fibronectin and Vitronectin Receptors Depends on Substrate Composition and Extracellular Matrix Accumulation, J Cell Biol 106:2171-2182.[Abstract/Free Full Text]
  • Smith, B.; Caffesse, R.; Nasjleti, C.; Kon, S.; and Castelli, W. (1987): Effects of Citric Acid and Fibronectin and Laminin Application in Treating Periodontitis, J Clin Periodontol 14:396-402.[CrossRef][Medline] [Order article via Infotrieve]
  • Sonnenberg, A.; Janssen, H.; Hogervorst, F.; Calafat, J.; and Hilgers, J. (1987): A Complex of Platelet Glycoproteins Ic and IIa Identified by a Rat Monoclonal Antibody, J Biol Chem 262:10376-10383.[Abstract/Free Full Text]
  • Sonnenberg, A.; Modderman, P.W.; and Hogervorst, F. (1988): Laminin Receptor on Platelets is the Integrin VLA-6, Nature 336:487-489.[CrossRef][Medline] [Order article via Infotrieve]
  • Terranova, V.P.; Franzetti, L.C.; Hic, S.; Diflorio, R.M.; Lyall, R.M.; Wikesjo, U.M.E.; Baker, P.J.; Christers-Son, L.A.; and Genco, R.J. (1986): A Biochemical Approach to Periodontal Regeneration: Tetracycline Treatment of Dentin Promotes Fibroblast Adhesion and Growth, J Periodont Res 21:330-337.[CrossRef][Medline] [Order article via Infotrieve]
  • Terranova, V.P. and Wikesjo, U.M.E. (1988): Chemotaxis of Cells Isolated from Periodontal Tissues to Different Biological Response Modifiers, Adv Dent Res 2:215-222.[Abstract/Free Full Text]
  • Wayner, E.A. and Carter, W.G. (1987): Identification of Multiple Cell Adhesion Receptors for Collagen and Fibronectin in Human Fibrosarcoma Cells Possessing Unique a and Common β Subunits, J Cell Biol 105:1873-1884.[Abstract/Free Full Text]
  • Wayner, E.A.; Carter, W.G.; Piotrowicz, R.S.; and Kunicki, T.J. (1988): The Function of Multiple Extracellular Matrix Receptors in Mediating Cell Adhesion to Extracellular Matrix: Preparation of Monoclonal Antibodies to the Fibronectin Receptor that Specifically Inhibit Cell Adhesion to Fibronectin and React with Platelet Glycoproteins Ic-IIa, J Cell Biol 107:1881-1891.[Abstract/Free Full Text]
  • Wayner, E.A.; Garcia-Pardo, A.; Humphries, M.J.; McDonald, J.A.; and Carter, W.G. (1989): Identification and Characterization of the T-Lymphocyte Adhesion Receptor for an Alternative Cell Attachment Domain (CS-1) in Plasma Fibronectin, J Cell Biol 109:1321-1330.[Abstract/Free Full Text]
  • Welch, M.P.; Odland, G.F.; and Clark, R.A.F. (1990): Temporal Relationships of F-Actin Bundle Formation, Collagen and Fibronectin Matrix Assembly, and Fibronectin Receptor Expression in Wound Contraction, J Cell Biol 110:133-145.[Abstract/Free Full Text]
  • Werb, Z.; Tremble, P.M.; Behrendtsen, 0.; Crowley, E.; and Damsky, C.H. (1989): Signal Transduction Through the Fibronectin Receptor Induces Collagenase and Stromelysin Gene Expression, J Cell Biol 109:887-889.
  • Ylanne, J. and Virtanen, I. (1989): The M, 140,000 Fibronectin Receptor Complex in Normal and Virus-transformed Human Fibroblasts and in Fibrosarcoma Cells: Identical Localization and Function, Int J Cancer 43:1126-1136.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 12, 1817-1823 (1990)
DOI: 10.1177/00220345900690120601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
S. M. Sheets, J. Potempa, J. Travis, C. A. Casiano, and H. M. Fletcher
Gingipains from Porphyromonas gingivalis W83 Induce Cell Adhesion Molecule Cleavage and Apoptosis in Endothelial Cells
Infect. Immun., March 1, 2005; 73(3): 1543 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
G. Thomas and P.M. Speight
Cell Adhesion Molecules and Oral Cancer
Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(6): 479 - 498.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Katz, V. Sambandam, J. H. Wu, S. M. Michalek, and D. F. Balkovetz
Characterization of Porphyromonas gingivalis-Induced Degradation of Epithelial Cell Junctional Complexes
Infect. Immun., March 1, 2000; 68(3): 1441 - 1449.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
J. M. Crawford and K. Watanabe
Cell Adhesion Molecules in Inflammation and Immunity: Relevance to Periodontal Diseases
Critical Reviews in Oral Biology & Medicine, January 1, 1994; 5(2): 91 - 123.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
M. Hormia, I. Virtanen, and V. Quaranta
Immunolocalization of Integrin {alpha}6{beta}4 in Mouse Junctional Epithelium Suggests an Anchoring Function to both the Internal and the External Basal Lamina
Journal of Dental Research, August 1, 1992; 71(8): 1503 - 1508.
[Abstract] [PDF]

Home page
 JDRHome page
V.-J. Uitto, H. Larjava, J. Peltonen, and D.M. Brunette
Expression of Fibronectin and Integrins in Cultured Periodontal Ligament Epithelial Cells
Journal of Dental Research, May 1, 1992; 71(5): 1203 - 1211.
[Abstract] [PDF]

Home page
 CROBMHome page
V.-J. Uitto and H. Larjava
Extracellular Matrix Molecules and their Receptors: An Overview with Special Emphasis on Periodontal Tissues
Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(3): 323 - 354.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Hormia, M.
Right arrow Articles by Virtanen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hormia, M.
Right arrow Articles by Virtanen, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?