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Journal of Dental Research
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Phenotypic Changes of Rabbit Mandibular Condylar Cartilage Cells in Culture

F.E. Engel

Department of Orthodontics The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78284

A.G. Khare

Department of Orthopedics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78284

B.D. Boyan

Department of Orthopedics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78284

The present study describes the behavior of mandibular condylar cartilage (MCC) cells as a function of time in primary culture, since it is not yet clear whether these cells maintain their phenotype in culture. MCC cells from New Zealand white rabbits were seeded at high density and cultured in DMEM containing 50 µg/mL ascorbic acid and 10% fetal bovine serum. These cells appeared as a heterogeneous population and changed their shape, size, and refractivity as cultures aged. Cartilage-like cells, which always dominated the culture, were infiltrated with a minority of fibroblast-like cells. Cell number increased progressively, and cultures reached confluence at nine days. Antibody activity for cartilage-specific glycosaminoglycan was determined by ELISA assay. This reaction reached a maximum at six days and decreased thereafter. Cultures stained with Alcian blue (pH 1.0) supported these results. Cytoplasmic mRNA analysis indicated that the transcription of type II collagen gene was present at all time points. Type I collagen and alkaline phosphatase mRNA levels showed progressive increases from 12 h to nine days, with significantly higher values in cells cultured for six, nine, and 12 days than in cells collected from earlier time points. These results suggest that in our present culture system, MCC cells undergo phenotypic changes that resemble their maturation processes in vivo.

REFERENCES

  • Adams, S.L., Pallante, K.M.; and Pacifici, M. (1989): Effects of Cell Shape on Type X Collagen Gene Expression in Hypertrophic Chondrocytes, Connect Tissue Res 20:223-232.[Medline] [Order article via Infotrieve]
  • Archer, C.W.; Rooney, P.; and Wolpert, L. (1982): Cell Shape and Cartilage Differentiation of Early Chick Limb Bud Cells in Culture, Cell Differ 11:245-261.[CrossRef][Medline] [Order article via Infotrieve]
  • Benya, F.D.; Padilla, S.R.; and Nimni, M.E. (1978): Independent Regulation of Collagen Types by Chondrocytes during the Loss of Differentiated Function in Culture, Cell 15:1313-1321.[CrossRef][Medline] [Order article via Infotrieve]
  • Blackwood, H.J.J. (1966): Growth of the Mandibular Condyle of the Rat Studied with Tritiated Thymidine, Arch Oral Biol 11:493-500.[CrossRef][Medline] [Order article via Infotrieve]
  • Boyan, B.D.; Schwartz, Z.; Swain, L.D.; Carnes, D.L.; and Zislis, T. (1988): Differential Expression of Phenotype by Resting Zone and Growth Region Costoehondral Chondrocytes in vitro, Bone 9:185-194.
  • Brigham, G.P.; Scaletta, L.J.; Johnston, L.E.; and Oc-Chino, J.C. (1977): Antigenic Differences among Condylar Epiphyseal and Nasal Septal Cartilage. In: The Biology of Occlusal Development, Monograph 7, Craniofacial Growth Series, J.A. McNamara, Ed., Ann Arbor: Center for Human Growth and Development, The University of Michigan, pp. 313-331.
  • Campo, R.D. and Romano, J.E. (1986): Changes in Cartilage Proteoglycans Associated with Calcification, Calcif Tissue Int 39:175-184.[Medline] [Order article via Infotrieve]
  • Castagnola, P.; Dozin, B.; Moro, G.; and Cancedda, R. (1988): Changes in the Expression of Collagen Genes Show Two Stages in Chondrocyte Differentiation in vitro, J Cell Biol 106:461-467.[Abstract/Free Full Text]
  • Charlier, J.P. and Petrovic, A.G. (1967): Recherche sur la Mandibule de Rat en Culture d'Organes: le Cartilage Condylien a-t-il un Potentiel de Croissance Independent, Orthodonthie Française 38:165-175.
  • Copray, J.C.V.M. and Duterloo, H.S. (1986): A Comparative Study on the Growth of Craniofacial Cartilages in vitro, Eur J Orthod 8:157-166.[Abstract/Free Full Text]
  • Copray, J.C.V.M.; Jansen, H.W.B.; and Duterloo, H.S. (1983): Growth of the Mandibular Condylar Cartilage of the Rat in Serumfree Organ Culture, Arch Oral Biol 28:967-974.[Medline] [Order article via Infotrieve]
  • Copray, J.C.V.M.; Jansen, H.W.B.; and Duterloo, H.S. (1985): The Role of Biomechanical Factors in Condylar Cartilage Growth and Remodeling in vitro. In: Developmental Aspects of Temporomandibular Joint Disorders, Monograph 16, Craniofacial Growth Series, D.S. Carlson, J.A. McNamara, and K.A. Ribbens, Eds., Ann Arbor: Center for Human Growth and Development, The University of Michigan, pp. 235-269.
  • Copray, J.C.V.M.; Jansen, H.W.B.; and Duterloo, H.S. (1986): Growth and Growth Pressure of Mandibular Condylar and some Primary Cartilages of the Rat in vitro, Am J Orthod Dentofac Orthop 90:19-28.[CrossRef][Medline] [Order article via Infotrieve]
  • Couchman, J.R.; Caterson, B.; Christner, J.E.; and Baker, J.R. (1984): Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues, Nature 307:650-652.[CrossRef][Medline] [Order article via Infotrieve]
  • Durkin, J.F.; Heeley, J.D.; and Irving, J.T. (1979): Cartilage of the Mandibular Condyle. In: Temporomandibular Joint Function and Dysfunction, G.A. Zarb and G.E. Carlson, Eds., St. Louis: C.V. Mosby Co., pp. 43-100.
  • Ebersole, J.L.; Taubman, M.A.; and Smith, D.J. (1985): Local Antibody Responses in Periodontal Diseases, J Periodontol 56 (Suppl. 11):51-55.[Medline] [Order article via Infotrieve]
  • Fallon, M.D.; Whyte, M.P.; and Teitelbaum, S.L. (1980): Stereospecific Inhibition of Alkaline Phosphatase by L-Tetramisole Prevents in vitro Calcification, Lab Invest 43:489-494.[Medline] [Order article via Infotrieve]
  • Folkman, J. and Moscona, A. (1978): Role of Cell Shape in Growth Control, Nature 273:345-349.[CrossRef][Medline] [Order article via Infotrieve]
  • Gerstenfeld, L.C.; Kelly, C.M.; Von Deck, M.; and Lian, J.D. (1990): Comparative Morphological and Biochemical Analysis of Hypertrophic, Non Hypertrophic and 1,25-dihydroxyvitamin D3 Treated Non Hypertrophic Chondrocytes, Connect Tissue Res 24:29-39.[Medline] [Order article via Infotrieve]
  • Gospodarowicz, D.; Greenburg, G.; and Birdwell, C.R. (1978): Determination of Cellular Shape by the Extracellular Matrix and its Correlation with the Control of Cellular Growth, Cancer Res 38:4155-4171.[Abstract/Free Full Text]
  • Kato, Y.; Iwamoto, M.; Koike, T.; Suzuki, F.; and Takano, Y. (1988): Terminal Differentiation and Calcification in Rabbit Chondrocyte Cultures Grown in Centrifuge Tubes: Regulation by Transforming Growth Factor β and Serum Factors, Proc Natl Acad Sci USA 85:9552-9556.[Abstract/Free Full Text]
  • Kuettner, K.E.; Pauli, B.U.; Gail, G.; Memoli, V.A.; and Schenk, R.K. (1982): Synthesis of Cartilage Matrix by Mammalian Chondrocytes in vitro. I. Isolation, Culture Characteristics and Morphology, J Cell Biol 93:743-750.
  • Leboy, P.S.; Oshima, O.; and Shapiro, I.M. (1990): Changes in Gene Expression and Metabolism in Chick Growth Cartilage, Connect Tissue Res 22:258.
  • McNamara, J.A., Jr. (1981): Functional Determinants of Craniofacial Size and Shape. In: Craniofacial Biology, Monograph 10, Craniofacial Growth Series, D.S. Carlson, Ed., Ann Arbor: Center for Human Growth and Development, The University of Michigan, pp. 181-221.
  • Meikle, M.C. (1973): In vivo Transplantation of the Mandibular Joint of the Rat; an Autoradiographic Investigation into Cellular Changes at the Condyle, Arch Oral Biol 18:1011-1020.[CrossRef][Medline] [Order article via Infotrieve]
  • Petrovic, A.G.; Stutzmann, J.J.; and Oudet, C.L. (1975): Control Processes in the Postnatal Growth of the Condylar Cartilage of the Mandible. In: Determinants of Mandibular Growth and Form, Monograph 4, Craniofacial Growth Series, J.A. McNamara, Ed., Ann Arbor: Center for Human Growth and Development, The University of Michigan, pp. 101-153.
  • Rifas, L.; Uitto, J.; Memoli, V.A.; Kuettner, K.E.; Henry, R.W.; and Peck, W.A. (1982): Selective Emergence of Differentiated Chondrocytes during Serumfree Culture of Cells Derived from Fetal Rat Calvaria, J Cell Biol 92:493-504.[Abstract/Free Full Text]
  • Rigby, D.W.J.; Dieckman, M.; Rhodes, C.; and Berg, P. (1977): Labeling Deoxyribonucleic Acid to High Specific Activity in vitro by Nick Translation with RNA Polymerase I, J Mol Biol 113:237-251.[CrossRef][Medline] [Order article via Infotrieve]
  • Schwartz, Z.; Schlader, D.L.; Ramirez, V.; Kennedy, M.B.; and Boyan, B.D. (1989): Effects of Vitamin D Metabolites on Collagen Production and Cell Proliferation of Growth Zone and Resting Zone Cartilage Cells in vitro, J Bone Min Res 4:199-207.[Medline] [Order article via Infotrieve]
  • Silbermann, M. and Frommer, J. (1973): Phosphatases within the Cartilage of the Mandibular Condyle of the Mouse, J Anat 116:335-345.[Medline] [Order article via Infotrieve]
  • Silbermann, M.; Reddi, H.; Hand, A.R.; Leapman, R.; Von Der Mark, K.; and Franzen, A. (1987a): Further Characterization of the Extracellular Matrix in the Mandibular Condylar Cartilage in Neonatal Mice, J Anat 151:169-188.[Medline] [Order article via Infotrieve]
  • Silbermann, M.; Tenenbaum, H.; Livne, E.; Leapman, R.; Von Der Mark, K.; and Reddi, A.H. (1987b): The in vitro Behavior of Fetal Condylar Cartilage in Serum-free Hormone-supplemented Medium, Bone 8:117-126.
  • Stutzman, J. and Petrovic, A. (1979): Intrinsic Regulation of the Condylar Cartilage Growth Rate, Europ J Orthod 1:41-54.
  • Suzuki, F.; Takase, T.; Takigawa, M.; Uchida, A.; and Shi-Momura, Y. (1981): Stimulation of the Initial Stage of Endochondral Ossification: in vitro Sequential Culture of Growth Cartilage Cells and Bone Marrow Cells, Proc Natl Acad Sci USA 78:2368-2372.[Abstract/Free Full Text]
  • Takano, T.; Takigawa, M.; Shirai, E.; Nakagawa, K.; Sakuda, M.; and Suzuki, F. (1987): The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Sphenooccipital Synchondrosis in Culture, J Dent Res 66:84-87.[Abstract/Free Full Text]
  • Takigawa, M.; Okada, M.; Takano, T.; Ohmae, H.; Sakuda, M.; and Suzuki, F. (1984): Studies on Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Sphenooccipital Synchondrosis in Culture. 1. Morphology, Growth, Glycosaminoglycan Synthesis and Responsiveness to Bovine Parathyroid Hormone (1-34), J Dent Res 63:19-22.[Abstract/Free Full Text]
  • Vaananen, H.K.; Morris, D.C.; and Anderson, H.C. (1983): Calcification of Cartilage Matrix in Chondrocyte Cultures Derived from Rachitic Rat Growth Plate Cartilage, Metab Bone Dis Rel Res 5:87-92.
  • Von Der Mark, K. and Conrad, G. (1979): Cartilage Cell Differentiation, Clin Orthop Rel Res 139:185-205.
  • Von Der Mark, K.; Gauss, V.; Von Der Mark, H.; and Muller, P. (1977): Relationship between Cell Shape and Type of Collagen Synthesized as Chondrocytes Lose their Cartilage Phenotype in Culture, Nature 267:531-532.[CrossRef][Medline] [Order article via Infotrieve]
  • Weiss, M.J.; Henthorn, P.S.; Lafferty, M.A.; Slaughter, C.; Raducha, M.; and Harris, H. (1986): Isolation and Characterization of a cDNA Encoding a Human Liver/Bone/Kidney-type Alkaline Phosphatase, Proc Natl Acad Sci USA 83:7182-7186.[Abstract/Free Full Text]
  • Weiss, A.; Livne, E.; Von Der Mark, K.; Heinegard, D.; and Silbermann, M. (1988): Growth and Repair of Cartilage: Organ Culture System Utilizing Chondroprogenitor Cells of Condylar Cartilage in Newborn Mice, J Bone Min Res 3:93-100.[Medline] [Order article via Infotrieve]
  • Weiss, A.; Von Der Mark, K.; and Silbermann, M. (1986): A Tissue Culture System Supporting Cartilage Cell Differentiation, Extracellular Mineralization and Subsequent Bone Formation, using Mouse Condylar Progenitor Cells, Cell Differ 19:103-113.[Medline] [Order article via Infotrieve]
  • White, B.A. and Bancroft, F.C. (1982): Cytoplasmic Dot Hybridization, J Biol Chem 257:8569-8572.[Abstract/Free Full Text]
  • Williams, G. and Jackson, D.S. (1956): Two Organic Fixatives for Acid Mucopolysaccharides, Stain Technology 31:189-191.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 11, 1753-1758 (1990)
DOI: 10.1177/00220345900690110801
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