Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kishimoto, E.
Right arrow Articles by Kent, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kishimoto, E.
Right arrow Articles by Kent, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Polymorphism of Submandibular-Sublingual Salivary Proteins which Promote Adhesion of Streptococcus mutans Serotype-c Strains to Hydroxyapatite

E. Kishimoto

Department of Preventive Dentistry, Okayama University Dental School, Shikata-cho, Okayama 700, Japan

D.I. Hay

Department of Biochemistry, Forsyth Dental Center, 140 Fenway, Boston, Massachusetts 02115

R. Kent

Department of Biostatistics, Forsyth Dental Center, 140 Fenway, Boston, Massachusetts 02115

Previously, we showed that human submandibular-sublingual (SMSL) salivas contain one or more proteins, Mr circa 300,000 daltons, which specifically promote adhesion of Streptococcus mutans serotype-c strains to hydroxyapatite. Also, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the adhesion-promoting proteins (APPs) exhibit heterogeneity. The aims of the present study were to determine whether APPs are generally present in human SMSL salivary secretions and to characterize the noted heterogeneity. Acid-stimulated SMSL saliva samples were obtained from 54 Japanese subjects, and M, values were obtained by SDS-PAGE. APPs were present in all saliva samples examined, though at significantly different concentrations. The APPs occurred as either single (20 subjects) or double bands (34 subjects), with a mean Mr (88 bands) of 297 kD and a range of 248-338 kD. A plot of the frequency distribution of the APPs according to Mr showed a trimodal distribution, with mean Mr values, standard deviations, and ranges for the three groups being 265 (S.D., 6.9; range, 248-278), 293 (S.D., 6.7; range, 280-305), and 320 (S.D., 7.0; range, 310-338) kD. Variations of Mr within groups may be attributed to experimental variation, although microheterogeneity cannot be excluded. Differences between groups can best be explained in terms of three polymorphic proteins, with low (L), intermediate (I), and high (H) Mr values. Six phenotypes were detected with L, I, H, LI, LH, and IH M r bands. A Hardy-Weinberg analysis showed that the phenotype data fit a single-gene, three-alleles model. The widespread occurrence of the APPs and their potential to modulate colonization of teeth by an oral pathogen suggest that the noted variations in concentrations and phenotypes may be of biological significance.

REFERENCES

  • Appelbaum, B.; Golub, E.; Holt, S.C.; and Rosan, B. (1979): In vitro Studies of Dental Plaque Formation: Adsorption of Oral Streptococci to Hydroxyapatite, Infect Immun 25:717-728.[Abstract/Free Full Text]
  • Azen, E.A. (1977): Salivary Peroxidase (SAPX): Genetic Modification and Relationship to the Proline-Rich (Pr) and Acidic (Pa) Proteins, Biochem Genet 15:9-29.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen, E.A. (1978): Genetic Protein Polymorphisms in Human Saliva: An Interpretive Review, Biochem Genet 16:79-99.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen, E.A. and Denniston, C.L. (1974): Genetic Polymorphism of Human Salivary Proline-rich Proteins: Further Genetic Analysis, Biochem Genet 12:109-120.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen, E.A.; Hurley, C.K.; and Denniston, C. (1979): Genetic Polymorphism of the Major Parotid Salivary Glycoprotein (Gl) with Linkage to the Genes for Pr, Db and Pa, Biochem Genet 17:257-279.[Medline] [Order article via Infotrieve]
  • Azen, E.A. and Maeda, N. (1988): Molecular Genetics of Human Salivary Proteins and their Polymorphisms. In: Advances in Human Genetics, Vol. 17, H. Harris and K. Hirshorn, Eds., New York: Plenum Press, Chapter 5, pp. 141-199.
  • Azen, E.A. and Oppenheim, F.G. (1973): Genetic Polymorphism of Proline-rich Human Salivary Proteins, Science 180:1067-1069.[Abstract/Free Full Text]
  • Babu, J.P. and Dabbous, M.K. (1986): Interaction of Salivary Fibronectin with Oral Streptococci, J Dent Res 65:1094-1100.[Abstract/Free Full Text]
  • Babu, J.P.; Simpson, W.A.; Courtney, H.S.; and Beachey, E.H. (1983): Interaction of Human Plasma Fibronectin with Cariogenic and Non-Cariogenic Oral Streptococci, Infect Immun 41:162-168.[Abstract/Free Full Text]
  • Clark, W.B.; Bammann, L.L.; and Gibbons, R.J. (1977): Comparative Estimate of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces, Infect Immun 19:846-853.
  • Douglas, C.W.I. and Russell, R.R.B. (1984): The Adsorption of Human Salivary Components to Strains of the Bacterium Streptococcus mutans, Arch Oral Biol 29:751-757.[CrossRef][Medline] [Order article via Infotrieve]
  • Ellen, R.P. (1985): Specificity of Attachment as a Tissue-Tropic Influence of Oral Bacteria. In: Molecular Basis of Oral Microbial Adhesion, S.E. Mergenhagen and B. Rosan, Eds., Washington, DC: Am Soc Microbiol, pp. 33-39.
  • Ericson, T. (1967): Adsorption to Hydroxyapatite of Proteins and Conjugated Proteins from Human Saliva, Caries Res 1:52-58.[Medline] [Order article via Infotrieve]
  • Ericson, T. (1968): Salivary Glycoproteins: Composition and Adsorption to Hydroxyapatite in Relation to the Formation of Dental Pellicles and Calculus, Acta Odontol Scand 26:3-21.[CrossRef][Medline] [Order article via Infotrieve]
  • Ericson, T. and Rundegren, J. (1983): Characterization of a Salivary Agglutinin Reacting with Serotype c Strain of Streptococcus mutans, Eur J Biochem 133:255-261.[Medline] [Order article via Infotrieve]
  • Gibbons, R.J. (1980): Adhesion of Bacteria to Surfaces in the Mouth. In: Microbial Adhesion to Surfaces, R.C.W. Berkeley, J.M. Lynch, J. Melling, P.R. Rutter, and B. Vincent, Eds., London: Soc. Chem. Industry, pp. 351-388.
  • Gibbons, R.J.; Cohen, L.; and Hay, D.I. (1986): Strains of Streptococcus mutans and Streptococcus sobrinus Attach to Different Pellicle Receptors, Infect Immun 52:555-561.[Abstract/Free Full Text]
  • Gibbons, R.J. and Hay, D.I. (1988): Human Salivary Acidic Proline-rich Proteins and Statherin Promote the Attachment of Actinomyces viscosus LY7 to Apatitic Surfaces, Infect Immun 56:439-445.[Abstract/Free Full Text]
  • Gibbons, R.J.; Moreno, E.C.; and Spinell, D.M. (1976): Model Delineating the Effects of a Salivary Pellicle on the Adsorption of Streptococcus mitior onto Hydroxyapatite, Infect Immun 14:1109-1112.[Abstract/Free Full Text]
  • Gibbons, R.J. and Qureshi, J.V. (1978): Selective Binding of Blood Group-reactive Salivary Mucins by Streptococcus mutans and Other Oral Organisms, Infect Immun 22:665-671.[Abstract/Free Full Text]
  • Gibbons, R.J. and Spinell, D.M. (1970): Salivary Induced Aggregation of Plaque Bacteria. In: Dental Plaque, W.D. McHugh, Ed., Edinburgh: E. & S. Livingstone, Inc., pp. 207-215.
  • Gibbons, R.J. and Van Houte, J. (1975): Bacterial Adherence in Oral Microbial Ecology, Ann Rev Microbiol 29:19-44.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons, R.J. and Van Houte, J. (1980): Bacterial Adherence and Formation of Dental Plaques. In: Bacterial Adherence, E.H. Beachey, Ed., London: Chapman and Hall, pp. 61-104.
  • Hay, D.I. (1967): The Adsorption of Salivary Proteins by Hydroxyapatite and Enamel, Arch Oral Biol 12:937-946.[CrossRef][Medline] [Order article via Infotrieve]
  • Hay, D.I. (1973): The Interactions of Human Parotid Salivary Proteins with Hydroxyapatite, Arch Oral Biol 18:1517-1529.[CrossRef][Medline] [Order article via Infotrieve]
  • Hay, D.I.; Gibbons, R.J.; and Spinell, D.M. (1971): Characteristics of some High Molecular Weight Constituents with Bacterial Aggregating Activity from Whole Saliva and Dental Plaque, Caries Res 5:111-123.[Medline] [Order article via Infotrieve]
  • Hillman, J.D.; Van Houte, J.; and Gibbons, R.J. (1970): Sorption of Bacteria to Human Enamel Powder, Arch Oral Biol 15:899-903.[CrossRef][Medline] [Order article via Infotrieve]
  • Kishimoto, E.; Hay, D.I.; and Gibbons, R.J. (1989): A Human Salivary Protein Which Promotes Adhesion of Streptococcus mutans Serotype c Strains to Hydroxyapatite, Infect Immun 57:3702-3707.[Abstract/Free Full Text]
  • Laemmli, U.K. (1970): Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature 277:680-685.
  • Leach, S.A.; Critchley, P.; Kolendo, A.B.; and Saxton, C.A. (1967): Salivary Glycoproteins as Components of the Enamel Integuments, Caries Res 1:104-111.[Medline] [Order article via Infotrieve]
  • Levine, M.J.; Herzberg, M.C.; Levine, M.S.; Ellison, S.A.; Stinson, M.W.; Li, H.C.; and Van Dyke, T. (1978): Specificity of Salivary-Bacterial Interactions: Role of Terminal Sialic Acid Residues in the Interaction of Salivary Glycoproteins with Streptococcus sanguis and Streptococcus mutans, Infect Immun 19:107-115.[Abstract/Free Full Text]
  • Malamud, D.; Christensen, C.M.; Navazesh, M.; and Davis, C. (1988): Bacterial Agglutinin Activity in the Saliva of Human Identical and Fraternal Twins, Arch Oral Biol 33:801-805.[CrossRef][Medline] [Order article via Infotrieve]
  • Mandel, I.D. (1978): Salivary Factors in Caries Prediction. In: Methods of Caries Prediction, B.G. Bibby and R.J. Shern, Eds., Spec Suppl, Microbial Abstracts, New York and London: Information Retrieval, Inc., pp. 147-158.
  • McGaughey, C.; Field, B.D.; and Stowell, E.C. (1971): Effects of Salivary Proteins on the Adsorption of Cariogenic Streptococci by Hydroxyapatite, J Dent Res 50:917-922.[Abstract/Free Full Text]
  • McGaughey, C. and Stowell, E.C. (1967): The Adsorption of Human Salivary Proteins and Porcine Submaxillary Mucin by Hydroxyapatite, Arch Oral Biol 12:815-828.[CrossRef][Medline] [Order article via Infotrieve]
  • Merritt, A.D.; Rivas, M.L.; Bixler, D.; and Newell, R. (1973): Salivary (Amy1) and Pancreatic (Amy2) Amylase: Electrophoretic Characterizations and Genetic Studies, Am J Hum Genet 25:510-522.[Medline] [Order article via Infotrieve]
  • Morrissey, J.H. (1981): Silver Stain for Proteins in Polyacrylamide Gels: A Modified Procedure with Enhanced Uniform Sensitivity, Anal Biochem 117:307-310.[CrossRef][Medline] [Order article via Infotrieve]
  • Murray, P.A.; Levine, M.J.; Tabak, L.A.; and Reddy, M.S. (1982): Specificity of Salivary-Bacterial Interactions: II. Evidence for a Lectin on Streptococcus sanguis with Specificity for a NeuAc-{alpha}-2,3-Gal-β-1,3-GaINAc Sequence, Biochem Biophys Res Commun 106:390-396.[CrossRef][Medline] [Order article via Infotrieve]
  • Prakobphol, A.; Levine, M.J.; Tabak, L.A.; and Reddy, M.S. (1982): Purification of a Low-Molecular Weight, Mucin-Type Glycoprotein from Human Submandibular-Sublingual Saliva, Carbohyd Res 108:111-122.[CrossRef][Medline] [Order article via Infotrieve]
  • Pruitt, K.M.; Caldwell, R.C.; Jamieson, A.D.; and Taylor, R.E. (1969): The Interaction of Salivary Proteins with Tooth Surface, J Dent Res 48:818-823.[Abstract/Free Full Text]
  • Rolla, G. (1969): The Adsorption of Radioactive Salivary Glycoprotein to Hydroxylapatite in vitro, J Periodont Res 4:165-166.[Medline] [Order article via Infotrieve]
  • Simpson, W.A.; Courtney, H.S.; and Ofek, I. (1987): Interactions of Fibronectin with Streptococci: The Role of Fibronectin as a Receptor for Streptococcus pyogenes, Rev Infect Dis 9 (Suppl. 4): S351-S359.
  • Slomiany, B.L.; Murty, V.L.N.; Mandel, I.D.; Zalesna, G.; and Slomiany, A. (1989): Physico-chemical Characteristics of Mucus Glycoproteins and Lipids of the Human Oral Mucosal Mucus Coat in Relation to Caries Susceptibility, Arch Oral Biol 34:229-237.[CrossRef][Medline] [Order article via Infotrieve]
  • Sonju, T. and Rolla, G. (1973): Chemical Analysis of the Acquired Pellicle Formed in Two Hours on Cleaned Human Teeth in vivo, Caries Res 7:30-38.[Medline] [Order article via Infotrieve]
  • SRB, A.M.; Owen, R.D.; and Edgar, R.S. (1965): General Genetics, 2nd ed., San Francisco: W.H. Freeman & Co., pp. 399-408.
  • Stinson, M.W.; Levine, M.J.; Cavese, J.M.; Prakobphol, A.; Murray, P.A.; Tabak, L.A.; and Reddy, M.S. (1982): Adherence of Streptococcus sanguis to Salivary Mucin Bound to Glass, J Dent Res 61:1390-1393.[Abstract/Free Full Text]
  • Tabak, L.A.; Levine, M.J.; Mandel, I.D.; and Ellison, S.A. (1982): Role of Salivary Mucins in the Protection of the Oral Cavity, J Oral Pathol 11:1-17.[Medline] [Order article via Infotrieve]
  • Tabak, L.A.; Levine, M.J.; Jain, N.K.; Bryan, A.R.; Cohen, R.E.; Monte, L.D.; Zawacki, S.; Nancollas, G.H.; Slomiany, A.; and Slomiany, B.L. (1985): Adsorption of Human Salivary Mucins to Hydroxyapatite, Arch Oral Biol 30:423-427.[CrossRef][Medline] [Order article via Infotrieve]
  • Van Houte, J. (1980): Bacterial Adhesion in the Mouth. In: Dental Plaque and Surface Interactions in the Oral Cavity, S.A. Leach, Ed., London: Information Retrieval, Limited, pp. 69-100.
  • Ward, J.C.; Merritt, A.D.; and Bixler, D. (1971): Human Salivary Amylase: Genetics of Electrophoretic Variants, Am J Hum Genet 23:403-409.[Medline] [Order article via Infotrieve]
  • Williams, R.C. and Gibbons, R.J. (1972): Inhibition of Bacterial Adherence by Secretory Immunoglobulin A: A Mechanism of Antigen Disposal, Science 177:696-699.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 69, No. 11, 1741-1745 (1990)
DOI: 10.1177/00220345900690110601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
A. Carlen, P. Bratt, C. Stenudd, J. Olsson, and N. Stromberg
Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces
Journal of Dental Research, January 1, 1998; 77(1): 81 - 90.
[Abstract] [PDF]

Home page
 ADRHome page
D.I. Hay
Salivary Factors in Caries Models
Advances in Dental Research, November 1, 1995; 9(3): 239 - 243.
[Abstract] [PDF]

Home page
 JDRHome page
A. Carlen and J. Olsson
Monoclonal Antibodies Against a High-molecular-weight Agglutinin Block Adherence to Experimental Pellicles on Hydroxyapatite and Aggregation of Streptococcus mutans
Journal of Dental Research, April 1, 1995; 74(4): 1040 - 1047.
[Abstract] [PDF]

Home page
 ADRHome page
C.W.I. Douglas
Bacterial-Protein Interactions in the Oral Cavity
Advances in Dental Research, July 1, 1994; 8(2): 254 - 262.
[Abstract] [PDF]

Home page
 CROBMHome page
F. A. Scannapieco
Saliva-Bacterium Interactions in Oral Microbial Ecology
Critical Reviews in Oral Biology & Medicine, January 1, 1994; 5(3): 203 - 248.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kishimoto, E.
Right arrow Articles by Kent, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kishimoto, E.
Right arrow Articles by Kent, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?