Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Azen, E.A.
Right arrow Articles by Warner, T.F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azen, E.A.
Right arrow Articles by Warner, T.F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

mRNAs for PRPs, Statherin, and Histatins in von Ebner's Gland Tissues

E.A. Azen

Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, Department of Medicine, University of Wisconsin, Madison, Wisconsin 53706

G. Hellekant

Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin 53706

L.M. Sabatini

Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706

T.F. Warner

Department of Pathology, University of Wisconsin, Madison, Wisconsin 53706

A search was made for expression of genes for proline-rich proteins (PRPs) and other salivary-type proteins, including statherin and histatins, in taste-bud tissues of mice and primates because of previous genetic findings in mice (Azen et al., 1986) that Prp and taste genes for certain bitter substances are either the same or closely linked. Taste-bud tissues and other tissues were tested for specific mRNAs with labeled DNA probes by Northern blotting and in situ hybridization. It was found that PRP mRNAs were present in von Ebner's glands of mice and macaques, and that there was a much greater degree of PRP mRNA induction in mouse parotid (16-fold) than in von Ebner's gland (two-fold) after in vivo isoproterenol stimulation. This difference may be due, in part, to differences in autonomic nerve innervation. Statherin and histatin mRNAs were found in macaque taste-bud tissues containing von Ebner's gland, and statherin protein was found in human von Ebner's gland by immunohistochemistry. The finding of PRP gene expression in von Ebner's gland, whose secretions have been suggested to play a role in taste stimulation, adds further support to a possible function of PRPs in bitter tasting. The possible functions of statherin and histatins in von Ebner's gland secretions may be related to statherin's regulation of salivary calcium and histatins' antibacterial and antifungal properties.

REFERENCES

  • Ann, D.K. and Carlson, D.M. (1985): The Structure and Organization of a Proline-rich Protein Gene of a Mouse Multigene Family, J Biol Chem 29:15863-15872.
  • Ann, D.K.; Clements, S.; Johnstone, E.M.; and Carlson, D.M. (1987): Induction of Tissue-specific Proline-rich Protein Multigene Families in Rat and Mouse Parotid Glands by Isoproterenol, J Biol Chem 15:899-904.
  • Ann, D.K.; Smith, M.K.; and Carlson, D.M. (1988): Molecular Evolution of the Mouse Proline-rich Protein Multigene Family, J Biol Chem 263:10887-10893.[Abstract/Free Full Text]
  • Avenet, P. and Lindemann, B. (1987): Patch-Clamp Study of Isolated Taste Receptor Cells of the Frog, J Membr Biol 97:223-240.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen, E.A. (1990): Linkage Studies of Genes for Salivary Proline-rich Proteins and Bitter Taste in Mouse and Man. In: Chemical Senses: Genetics of Perception and Communication, C.J. Wysocki and M.R. Kare, Eds., New York: Marcel-Dekker.
  • Azen, E.A.; Davisson, T.M.; Cherry, M.; and Taylor, B.A. (1989): Prp (Proline-Rich Protein) Genes Linked to Markers Es-12 (Esterase-12), Ea-10 (Erythrocyte Alloantigen) and Loci on Distal Mouse Chromosome 6, Genomics 5:415-422.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen, E.A.; Lush, I.E.; and Taylor, B.A. (1986): Close Linkage of Mouse Genes for Salivary Proline-rich Proteins (PRPs) and Taste, Trends in Genetics 2:199-200.
  • Azen, E.A.; Lyons, K.M.; McGonigal, T.; Barrett, N.L.; Clements, L.S.; Maeda, N.; Vanin, E.F.; Carlson, D.M.; and Smithies, O. (1984): Clones From the Human Gene Complex Coding for Salivary Proline-rich Proteins, Proc Natl Acad Sci USA 81:5561-5565.[Abstract/Free Full Text]
  • Bennick, A. (1982): Salivary Proline-rich Proteins, Mol Cell Biochem 45:83-99.[Medline] [Order article via Infotrieve]
  • Burstone, M.S. (1956): Esterase of the Salivary Glands, J Histochem Cytochem 4:130-139.[Medline] [Order article via Infotrieve]
  • Dickinson, D.P.; Ridall, A.L.; and Levine, M.J. (1987): Human Submandibular Gland Statherin and Basic Histidine-rich Peptide are Encoded by Highly Abundant mRNAs Derived From a Common Ancestral Sequence, Biochem Biophys Res Commun 149:784-790.[Medline] [Order article via Infotrieve]
  • Feinberg, A.P. and Vogelstein, B. (1983): A Technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Acitivity, Anal Biochem 132:6-13.[CrossRef][Medline] [Order article via Infotrieve]
  • Gurkan, S. and Bradley, R.M. (1987): Autonomic Control of von Ebner's Lingual Salivary Glands and Implications for Taste Sensation, Brain Res 419:287-293.[CrossRef][Medline] [Order article via Infotrieve]
  • Gurkan, S. and Bradley, R.M. (1988): Effects of Electrical Stimulation of Autonomic Nervous System on Degranulation of von Ebner's Gland Acini, Brain Res 473:127-133.[Medline] [Order article via Infotrieve]
  • Hamosh, M. and Scow, R.O. (1973): Lingual Lipase and its Role in the Digestion of Dietary Lipid, J Clin Invest 52:88-95.[Medline] [Order article via Infotrieve]
  • Hay, D.I.; Smith, D.J.; Schluckebier, S.K.; and Moreno, E.C. (1984): Relationship Between Concentration of Human Salivary Statherin and Inhibition of Calcium Phosphate Precipitation in Stimulated Human Parotid Saliva, J Dent Res 63:857-863.[Abstract/Free Full Text]
  • Kalmus, H. (1971): Genetics of Taste. In: Handbook of Sensory Physiology, Vol. IV, Chemical Senses, Part 2, L.M. Beidler, Ed., Berlin: Springer-Verlag, pp. 165-179.
  • Kim, H.-S. and Maeda, N. (1986): Structures of Two HaeIII-Type Genes in the Salivary Proline-rich Protein Multigene Family, J Biol Chem 261:6712-6718.[Abstract/Free Full Text]
  • Kinnamon, S.C. and Roper, S.D. (1988): Membrane Properties of Isolated Mudpuppy Taste Cells, J Gen Physiol 91:351-371.[Abstract/Free Full Text]
  • Lush, I.E. and Holland, G. (1988): The Genetics of Tasting in Mice. V. Glycine and Cycloheximide, Genet Res Camb 52:207-212.[Medline] [Order article via Infotrieve]
  • Mehansho, H.; Butler, L.G.; and Carlson, D.M. (1987): Dietary Tannins and Salivary Proline-rich Proteins: Interactions, Induction and Defense Mechanisms. In: Annual Review of Nutrition, Vol. 7, R.E. Olson, E. Beutler, and H.P. Broquist, Eds., Palo Alto, California: Annual Reviews, Inc., pp. 423-440.
  • Mehansho, H.; Clements, S.; Sheares, B.T.; Smith, S.; and Carlson, D.M. (1985): Induction of Proline-rich Glycoprotein Synthesis in Mouse Salivary Glands by Isoproterenol and by Tannins, J Biol Chem 260:4418-4423.[Abstract/Free Full Text]
  • Minaguchi, K. and Bennick, A. (1989): Genetics of Human Salivary Proteins, J Dent Res 68:2-15.[Free Full Text]
  • Mira, E. (1965): Cytochemical Localization of Oxidative and Hydrolytic Enzymes in von Ebner's Glands, Acta Oto-Laryngol 59:88-96.[Medline] [Order article via Infotrieve]
  • Miyamoto, T.; Okada, Y.; and Sato, T. (1988): Ionic Basis of Receptor Potential of Frog Taste Cells Induced by Acid Stimuli, J Physiol Lond 405:699-711.[Abstract/Free Full Text]
  • O'Hanlon, K.; Weissbecker, K.; Cortessis, V.; Spence, M.A.; and Azen, E.A. (1989): Genes for Salivary Proline-rich Proteins and Taste for Phenylthiourea are not Closely Linked in Humans, Cytogenet Cell Genet 49:315-317.
  • Okada, Y.; Miyamoto, T.; and Sato, T. (1986): Contribution of the Receptor and Basolateral Membranes to the Resting Potential of a Frog Taste Cell, Jpn J Physiol 36:139-150.[Medline] [Order article via Infotrieve]
  • Sabatini, L.M. and Azen, E.A. (1989): Histatins, a Family of Salivary Histidine-rich Proteins, are Encoded by at Least Two Loci (His1 and His2), Biochem Biophys Res Commun 106:495-502.
  • Sabatini, L.M.; Carlock, L.R.; Johnson, G.W.; and Azen, E.A. (1987): cDNA Cloning and Chromosomal Localization of a Gene for Statherin, a Regulator of Calcium in Saliva, Am J Hum Genet 41:1048-1060.[Medline] [Order article via Infotrieve]
  • Sabatini, L.M.; Warner, T.F.; Saitoh, E.; and Azen, E.A. (1989): Tissue Distributions of RNAs for Cystatins, Histatins, Statherin, and Proline-rich Salivary Proteins in Humans and Macaques, J Dent Res 68:1138-1145.
  • Schlesinger, D.H.; Buku, A.; Wyssbrod, H.R.; and Hay, D.I. (1987): Chemical Synthesis of Phosphoseryl-Phosphoserine, a Partial Analogue of Human Salivary Statherin,.a Protein Inhibitor of Calcium Phosphate Precipitation in Human Saliva, Int J Pept Protein Res 30:257-262.[Medline] [Order article via Infotrieve]
  • Schlesinger, D.H. and Hay, D.I. (1986): Complete Covalent Structure of a Proline-rich Phosphoprotein, PRP-2, an Inhibitor of Calcium Phosphate Crystal Growth from Human Parotid Saliva, Int J Pept Protein Res 27:373-379.[Medline] [Order article via Infotrieve]
  • Schmale, H.; Holtgreve-Grez, H.; and Christiansen, H. (1990): Possible Role of Salivary Gland Protein in Taste Reception Indicated by Homology to Lipophilic-Ligand Carrier Proteins, Nature 343:366-399.[CrossRef][Medline] [Order article via Infotrieve]
  • Shingai, T. and Beidler, L.M. (1985): Interstrain Differences in Bitter Taste Responses in Mice, Chemical Senses 10:51-55.[Abstract/Free Full Text]
  • Taylor, T. and Erlandsen, S.L. (1973): Peroxidase Localization in von Ebner's Gland in Man, J Dent Res 52:635.[Free Full Text]
  • Tonosaki, K. and Funakoshi, M. (1988): Cyclic Nucleotides May Mediate Taste Transduction, Nature 331:354-356.[CrossRef][Medline] [Order article via Infotrieve]
  • Tremblay, G. and Charest, J. (1968): Modified Starch Gel Film Method for the Histochemical Localization of Amylase Activity, J Histochem Cytochem 16:147-148.[Medline] [Order article via Infotrieve]
  • Whitney, G. and Harder, D.B. (1986): Phenylthiocarbamide (PTC) Preference among Laboratory Mice: Understanding of a Previous "Unreplicated" Report, Behavior Genetics 16:605-610.[CrossRef][Medline] [Order article via Infotrieve]
  • Ziemer, M.A.; Swain, W.F.; Rutter, W.J.; Clements, S.; Ann, D.K.; and Carlson, D.M. (1984): Nucleotide Sequence Analysis of a Proline-rich Protein cDNA and Peptide Homologies of Rat and Human Proline-rich Proteins, J Biol Chem 259:10475-10480.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 69, No. 11, 1724-1730 (1990)
DOI: 10.1177/00220345900690110401
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
M. Ahmad, M. Piludu, F. G. Oppenheim, E. J. Helmerhorst, and A. R. Hand
Immunocytochemical Localization of Histatins in Human Salivary Glands
J. Histochem. Cytochem., March 1, 2004; 52(3): 361 - 370.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
H. Tsai and L.A. Bobek
Human Salivary Histatins: Promising Anti-Fungal Therapeutic Agents
Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(4): 480 - 497.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
L. C.P.M. Schenkels, E. C.I. Veerman, and A. V. Nieuw Amerongen
Biochemical Composition of Human Saliva in Relation To Other Mucosal Fluids
Critical Reviews in Oral Biology & Medicine, January 1, 1995; 6(2): 161 - 175.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
D. K. Ann and H. H. Lin
Macaque Salivary Proline-Rich Protein: Structure, Evolution, and Expression
Critical Reviews in Oral Biology & Medicine, January 1, 1993; 4(3): 545 - 551.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Azen, E.A.
Right arrow Articles by Warner, T.F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azen, E.A.
Right arrow Articles by Warner, T.F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?