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An Intra-oral Appliance Study of the Plaque Microflora Associated with Early Enamel Demineralization
L.M.D. Macpherson
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
T.W. MacFarlane
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
K.W. Stephen
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
An intra-oral appliance model was used to investigate the composition of the plaque microflora associated with early enamel demineralization. Enamel sections, with exposed windows, were mounted on lower removable appliances, and the devices were worn by volunteers for three-week periods under three experimental conditions. These were: (1) "normal" plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each volunteer's own mutans streptococci onto the test sites and sucrose applications as described for (2). After 21 days, the plaque overlying each window was removed, and the bacterial composition was determined. Changes in mineral content of the associated enamel were measured by microradiography and microdensitometry, and the total mineral loss (Az) that had occurred at each site was calculated. The 144 sites studied were divided into four demineralization groups by Az value, with an increase in mineral loss from group 1 to group 4.
A progressive and significant increase in the isolation frequency of mutans streptococci occurred from Az group 1 to group 4 sites. These organisms were isolated from the plaque of every location with enamel mineral loss of over 1000 Az units, but were not detected in 27% of the group 3 sites. Lactobacilli comprised 2% to 3% of the total cultivable microflora in groups 1-3 sites, but were found in significantly higher proportions (18%) at those enamel sites experiencing the most extensive mineral loss (group 4). No significant relationship was found between demineralization and the levels of Actinomyces species or Veillonella.
This model allows plaque bacterial composition to be related to the very early stages of enamel demineralization, and the results support the view that lactobacilli are associated with the more established lesions.
REFERENCES
- Arends, J. and Christoffersen, J. (1986): The Nature of Early Caries Lesions in Enamel, J Dent Res 65:2-11.[Abstract/Free Full Text]
- Bowden, G.H.; Hardie, J.M.; McKee, A.S.; Marsh, P.D.; Fillery, E.D.; and Slack, G.L. (1976): The Microflora Associated with Developing Carious Lesions of the Distal Surfaces on the Upper First Premolars in 13-14 Year Old Children. In: Microbial Aspect of Dental Caries, Vol. 1, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Sp. Supp. Microbiology Abstracts, Washington, D.C.: Information Retrieval, Inc., pp. 223-242.
- Boyar, R.M. and Bowden, G.H. (1985): The Microflora Associated with the Progression of Incipient Carious Lesions in Teeth of Children Living in a Water-fluoridated Area, Caries Res 19:298-306.[Medline]
[Order article via Infotrieve]
- Boyar, R.M.; Thylstrup, A.; Holmen, L.; and Bowden, G.H. (1989): The Microflora Associated with the Development of Initial Enamel Decalcification below Orthodontic Bands in vivo in Children Living in a Fluoridated-water Area, J Dent Res 68:1734-1738.[Abstract/Free Full Text]
- Carlsson, P.; Olsson, B.; and Bratthall, D. (1985): The Relationship Between the Bacterium Streptococcus mutans in the Saliva and Dental Caries in Children in Mozambique, Arch Oral Biol 30:265-268.[CrossRef][Medline]
[Order article via Infotrieve]
- De Groot, J.F.; Borggreven, J.M.P.M.; and Driessens, F.C.M. (1986): Some Aspects of Artificial Caries Lesion Formation of Human Dental Enamel in vitro, J Biol Buccale 14:125-131.[Medline]
[Order article via Infotrieve]
- Duchin, S. and Van Houte, J. (1978): Relationship of Streptococcus mutans and Lactobacilli to Incipient Smooth Surface Dental Caries in Man, Arch Oral Biol 23:779-786.[CrossRef][Medline]
[Order article via Infotrieve]
- Emilson, C.G. and Krasse, B. (1985): Support for and Implications of the Specific Plaque Hypothesis, Scand J Dent Res 93:96-104.[Medline]
[Order article via Infotrieve]
- Gallagher, I.H.C. and Pearce, E.I.F. (1979): The Microbiology of Accelerated Experimental Dental Caries in Man, J Dent Res (Sp Iss D): No. 35.
- Hardie, J.M.; Thomson, P.L.; South, R.J.; Marsh, P.D.; Bowden, G.H.; McKee, A.S.; Fillery, E.D.; and Slack, G.L. (1977): A Longitudinal Epidemiological Study on Dental Plaque and the Development of Caries-Interim Results After Two Years, J Dent Res 56 (Sp Iss C): C90-C98.[Free Full Text]
- Holmen, L.; Thylstrup, A.; Øgaard, B.; and Kragh, F. (1985): A Polarized Light Microscopic Study of Progressive Stages of Enamel Caries in vivo, Caries Res 19:348-354.[Medline]
[Order article via Infotrieve]
- Koulourides, T.; Bodden, R.; Keller, S.; Manson-Hing, L.; Lastra, J.; and Housch, T. (1976): Cariogenicity of Nine Sugars Tested with an Intraoral Device in Man, Caries Res 10:427-441.[Medline]
[Order article via Infotrieve]
- Lang, N.P.; Hotz, P.R.; Gusberti, F.A.; and Joss, A. (1987): Longitudinal Clinical and Microbiological Study on the Relationship Between Infection with Streptococcus mutans and the Development of Caries in Humans, Oral Microbiol Immunol 2:39-47.[CrossRef][Medline]
[Order article via Infotrieve]
- Loesche, W.J.; Eklund, S.; Earnest, R.; and Burt, B. (1984): Longitudinal Investigation of Bacteriology of Human Fissure Decay: Epidemiological Studies in Molars Shortly After Eruption, Infect Immun 46:765-772.[Abstract/Free Full Text]
- Loesche, W.J.; Rowan, J.; Straffon, L.H.; and Loos, P.J. (1975): Association of Streptococcus mutans with Human Dental Decay, Infect Immun 11:1252-1260.[Abstract/Free Full Text]
- Loesche, W.J. and Straffon, L.H. (1979): Longitudinal Investigation of the Role of Streptococcus mutans in Human Fissure Decay, Infect Immun 26:498-507.[Abstract/Free Full Text]
- Macpherson, L.M.D.; MacFarlane, T.W.; Aitchison, T.C.; and Stephen, K.W. (1990): The Effect of Sucrose Application and Implantation of Mutans Streptococci on the Microbial Composition of Three-week Experimental Plaque-An in situ Study J Dent Res 69:1706-1711.
- Marsh, P.D.; Featherstone, A.; McKee, A.S.; Halls-Worth, A.S.; Robinson, C.; Weatherell, J.A.; Newman, H.N.; and Pitfer, A.F.V. (1989): A Microbiological Study of Early Caries of Approximal Surfaces in Schoolchildren, J Dent Res 68:1151-1154.[Abstract/Free Full Text]
- Meiers, J.C.; Wirthlin, M.R.; and Shklair, I.L. (1982): A Microbiological Analysis of Human Early Carious and Non-carious Fissures, J Dent Res 61:460-464.[Abstract/Free Full Text]
- Milnes, A.R. and Bowden, G.H.W. (1985): The Microflora Associated with Developing Lesions of Nursing Caries, Caries Res 19:289-297.[Medline]
[Order article via Infotrieve]
- Ostrom, C.A.; Koulourides, T.; Hickman, F.; and McGhee, J.R. (1977): Microbial Characterization of an Experimental Cariogenic Plaque in Man, J Dent Res 56:550-558.[Abstract/Free Full Text]
- Strang, R.; Damato, F.A.; Creanor, S.L.; and Stephen, K.W. (1987): The Effect of Baseline Lesion Mineral Loss on in situ Remineralization, J Dent Res 66:1644-1646.[Abstract/Free Full Text]
- Strang, R.; Damato, F.A.; and Stephen, K.W. (1988): Comparison of in vitro Demineralization of Enamel Sections and Slabs, Caries Res 22:348-349.[Medline]
[Order article via Infotrieve]
- Van Houte, J.; Gibbons, R.J.; and Pulkkinen, A.J. (1972): Ecology of Human Oral Lactobacilli, Infect Immun 6:723-729.[Abstract/Free Full Text]
- Walter, R.G. (1982): A Longitudinal Study of Caries Development in Initially Caries-free Naval Recruits, J Dent Res 61:1405-1407.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 69, No. 11,
1712-1716 (1990)
DOI: 10.1177/00220345900690110201
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