|
Sign In to gain access to subscriptions and/or personal tools.
|
The Effect of Sucrose Application and Implantation of Mutans Streptococci on the Microbial Composition of Three-week Experimental Plaque—An in situ Study
L.M.D. Macpherson
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
T.W. MacFarlane
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
T.C. Aitchison
Department of Statistics, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, United Kingdom
K.W. Stephen
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom
This study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. Lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. These were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans streptococci onto the enamel test sites and sucrose applications, as described above. With the exception of slightly higher proportions of Gram-negative bacilli associated with slab plaque following sucrose application, no significant differences in percentage or absolute counts of organisms were found between normal and sucrose plaques. The inoculation of mutans streptococci, combined with extra-oral sucrose applications, was associated with significantly higher percentages and absolute mean counts of both mutans streptococci and lactobacilli, and lower proportions of S. sanguis and S. oralis. Although the isolation frequency of mutans streptococci increased in all subjects and the overall mean proportion rose following inoculation, considerable inter-subject variation was seen in mean percentage counts of these organisms isolated from the three-week plaque samples.
REFERENCES
- Arneberg, P.; Øgaard, B.; Scheie, A.A.; and Rolla, G. (1984): Selection of Streptococcus mutans and Lactobacilli in an Intra-oral Caries Model, J Dent Res 63:1197-1200.[Abstract/Free Full Text]
- Bibby, B.G. (1976): Influence of Diet on the Bacterial Composition of Plaques. In: Microbial Aspects of Dental Caries, Vol II, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Washington, D.C.: Information Retrieval, Inc., pp. 477-490.
- Borden, L.W.; Ostrom, C.A.; and Koulourides, T. (1980): Establishment of Potentially Cariogenic Streptococci in an Experimental Human Plaque. I: Streptococcus mutans, J Dent Res 59:588-593.[Free Full Text]
- Bowden, G.H.; Hardie, J.M.; and Slack, G.L. (1975): Microbial Variations in Approximal Dental Plaque, Caries Res 9:253-277.[Medline]
[Order article via Infotrieve]
- Brudevold, F.; Attarzadeh, F.; Tehrani, A.; Van Houte, J.; and Russo, J. (1984): Development of a New Intraoral Demineralization Test, Caries Res 18:421-429.[Medline]
[Order article via Infotrieve]
- Creanor, S.L.; Strang, R.; Telfer, S.; MacDonald, I.; Smith, M.J.; and Stephen, K.W. (1986): In situ Appliance for the Investigation of Enamel De- and Remineralisation. A Pilot Study, Caries Res 20:385-391.
- Edwardsson, S. (1986): Microorganisms Associated with Dental Caries. In: Textbook of Cariology, A. Thylstrup and O. Fejerskov, Eds., Copenhagen: Munksgaard, pp. 107-130.
- Emilson, C.G. and Krasse, B. (1985): Support for and Implications of the Specific Plaque Hypothesis, Scand J Dent Res 93:96-104.[Medline]
[Order article via Infotrieve]
- Folke, L.E.A.; Gawronski, T.H.; Staat, R.H.; and Harris, R.S. (1972): Effect of Dietary Sucrose on Quantity and Quality of Plaque, Scand J Dent Res 80:529-533.[Medline]
[Order article via Infotrieve]
- Geddes, D.A.M.; Cooke, J.A.; Edgar, W.M.; and Jenkins, G.N. (1978): The Effect of Frequent Sucrose Mouthrinsing on the Induction in vivo of Caries-like Changes in Human Dental Enamel, Arch Oral Biol 23:663-665.[Medline]
[Order article via Infotrieve]
- Hamada, S. and Slade, H.D. (1980): Biology, Immunology and Cariogenicity of Streptococcus mutans, Microbiol Rev 44:331-384.[Free Full Text]
- Holmen, L.; Thylstrup, A.; Øgaard, B.; and Kragh, F. (1985): A Polarized Light Microscopic Study of Progressive Stages of Enamel Caries in vivo, Caries Res 19:348-354.[Medline]
[Order article via Infotrieve]
- Jordan, H.V.; Englander, H.R.; Engler, W.O.; and Kul-Czyk, S. (1972): Observations on the Implantation and Transmission of Streptococcus mutans in Humans, J Dent Res 51:515-518.[Abstract/Free Full Text]
- Macpherson, L.M.D.; MacFarlane, T.W.; and Stephen, K.W. (1990): An Intra-oral Appliance Study of the Plaque Microflora Associated with Early Enamel Demineralization, J Dent Res 69:1712-1716.[Abstract/Free Full Text]
- Ostrom, C.A.; Koulourides, T.; Hickman, F.; and McGhee, J.R. (1977): Microbial Characterization of an Experimental Cariogenic Plaque in Man, J Dent Res 56:550-558.[Abstract/Free Full Text]
- Rugg-Gunn, A.J. (1983): Diet and Dental Caries. In: The Prevention of Dental Disease, J.J. Murray, Ed., Oxford: Oxford Medical Publications, pp. 3-82.
- Scheie, A.A.; Arneberg, P.; Rstavik, D.; and Afseth, J. (1984): Microbial Composition, pH-depressing Capacity and Acidogenicity of 3-week Smooth Surface Plaque Developed on Sucrose-regulated Diets in Man, Caries Res 18:74-86.[Medline]
[Order article via Infotrieve]
- Schmidhuber, S.; Kilpper-Bals, R.; and Schleifer, K.H. (1987): A Taxonomic Study of Streptococcus mitis, S. oralis and S. sanguis, Syst Applied Microbiol 10:74-77.
- Shklair, I.L. and Keene, H.J. (1974): A Biochemical Scheme for the Separation of the Five Varieties of Streptococcus mutans, Arch Oral Biol 19:1079-1081.[CrossRef][Medline]
[Order article via Infotrieve]
- Staat, R.H.; Gawronski, T.H.; Cressey, D.E.; Harris, R.S.; and Folke, L.E.A. (1975): Effect of Dietary Sucrose Levels on the Quantity and Microbial Composition of Human Dental Plaque, J Dent Res 54:872-880.[Abstract/Free Full Text]
- Strang, R.; Damato, F.A.; and Stephen, K.W. (1988): Comparison of in vitro Demineralization of Enamel Sections and Slabs, Caries Res 22:348-349.[Medline]
[Order article via Infotrieve]
- Svanberg, M. and Krasse, B. (1981): Oral Implantation of Saliva-treated Streptococcus mutans in Man, Arch Oral Biol 26:197-201.[CrossRef][Medline]
[Order article via Infotrieve]
- Svanberg, M.L. and Loesche, W.J. (1978): Intraoral Spread of Streptococcus mutans in Man, Arch Oral Biol 23:557-561.[CrossRef][Medline]
[Order article via Infotrieve]
- Syed, S.A. and Loesche, W.J. (1978): Bacteriology of Human Experimental Gingivitis: Effect of Plaque Age, Infect Immun 21:821-829.[Abstract/Free Full Text]
- Tehrani, A.; Goulet, D.; Brudevold, F.; and Attarzadeh, F. (1986): Enamel Demineralization and the Length of Intra-oral Exposure to Different Concentrations of Glucose or Sucrose, J Dent Res 65:139-145.[Abstract/Free Full Text]
- Van Houte, J. (1980): Bacterial Specificity in the Etiology of Dental Caries, Int Dent J 30:305-326.[Medline]
[Order article via Infotrieve]
- Von Der Fehr, F.R.; Löe, H.; and Theilade, E. (1970): Experimental Caries in Man, Caries Res 4:131-148.[Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 69, No. 11,
1706-1711 (1990)
DOI: 10.1177/00220345900690110101
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
G.H.W. Bowden and Y.H. Li
Nutritional Influences on Biofilm Development
Advances in Dental Research,
April 1, 1997;
11(1):
81 - 99.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C.H. Sissons
Artificial Dental Plaque Biofilm Model Systems
Advances in Dental Research,
April 1, 1997;
11(1):
110 - 126.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P.D. Marsh
The Role of Microbiology in Models of Dental Caries
Advances in Dental Research,
November 1, 1995;
9(3):
244 - 254.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.M. ten Cate and P.D. Marsh
Procedures for Establishing Efficacy of Antimicrobial Agents for Chemotherapeutic Caries Prevention
Journal of Dental Research,
March 1, 1994;
73(3):
695 - 703.
[Abstract]
[PDF]
|
|
|
|
|
