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Journal of Dental Research
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Changes in Protein Secretion by Rat Submandibular Glands in Response to Isoproterenol, a-Methylnoradrenaline, and Clonidine during Post-natal Development

E. Tanaka

The First Department of Prosthodontics and Department of Oral Biochemistry, Fukuoka Dental College, 700, Ta, Sawara-ku, Fukuoka-shi, 814-01, Japan

T. Habu

The First Department of Prosthodontics and Department of Oral Biochemistry, Fukuoka Dental College, 700, Ta, Sawara-ku, Fukuoka-shi, 814-01, Japan

A. Letic-Gavrilovic

The First Department of Prosthodontics and Department of Oral Biochemistry, Fukuoka Dental College, 700, Ta, Sawara-ku, Fukuoka-shi, 814-01, Japan

K. Abe

The First Department of Prosthodontics and Department of Oral Biochemistry, Fukuoka Dental College, 700, Ta, Sawara-ku, Fukuoka-shi, 814-01, Japan

We studied developmental changes in salivary volumes and proteins secreted by the submandibular glands of male rats at weekly intervals from two to ten weeks of age in response to the β1-, {alpha}1-, and {alpha}2- adrenoceptor agonists, isoproterenol (IPR), a-methylnoradrenaline (a-mNA), and clonidine (Clonid). The types of proteins in saliva samples were determined and compared by isoelectric-focusing electrophoresis with the Phast system in both the gradient pH -3.5-to-5 and pH-3.5-to-9 gels by means of silver staining. Salivary volume and protein concentration in saliva samples elicited by IPR and a-mNA were positively related to the weight of the submandibular glands up to six or seven weeks of age, whereas in saliva elicited by Clonid, no relation was found. The isoelectric-focusing electrophoretic patterns of proteins secreted by the glands in response to three stimuli were different from each other during post-natal development. Within one stimulation, differences were also observed at two and three weeks of age for Clonid, and from seven weeks of age for the three stimuli, respectively. The a-type proteins, but not the β-type proteins, were very similar to those in extracts from glands of rats at seven weeks of age. Almost all of the a-type proteins, but not the β-type proteins, reacted with antibodies to two proteases. We conclude that functional maturation precedes morphological maturation in the submandibular glands of rats.

REFERENCES

  • Abe, K. (1987): Autonomic Effects on Protein Secretion by Rat Submandibular Salivary Glands, Comp Biochem Physiol 88C:241-248.
  • Abe, K. and Dawes, C. (1978): The Effects of Electrical and Pharmacological Stimulation on the Types of Proteins Secreted by Rat Parotid and Submandibular Glands, Arch Oral Biol 23:367-372.[CrossRef][Medline] [Order article via Infotrieve]
  • Abe, K. and Dawes, C. (1980): The Secretion of Protein and of Some Electrolytes in Response to a- and (3-adrenergic Agonists by Rat Parotid and Submandibular Salivary Glands Enlarged by Chronic Treatment with Isoproterenol, J Dent Res 59:1081-1089.[Abstract/Free Full Text]
  • Abe, K. and Dawes, C. (1982): Dopamine-induced Secretion of Protein and of Some Electrolytes by Rat Submandibular and Parotid Glands, Arch Oral Biol 27:635-643.[CrossRef][Medline] [Order article via Infotrieve]
  • Abe, K. and Dawes, C. (1984): The Effects of {alpha}-methylnoradrenaline on Protein and Electrolyte Secretion by Rat Submandibular and Parotid Glands, Comp Biochem Physiol 78C:383-389.
  • Abe, K. and Dawes, C. (1985): The Effects of Oxymetazoline on Secretion of Protein and Some Electrolytes by Rat Submandibular and Parotid Glands, Comp Biochem Physiol 80C:347-353.[CrossRef][Medline] [Order article via Infotrieve]
  • Abe, K.; Hidaka, S.; Ishibashi, K.; Yanabu, M.; Kamogashira, K.; Itoh, T.; and Matsumoto, M. (1987): Developmental Changes in the Volumes, Protein, and Some Electrolyte Concentrations of Male and Female Rat Submandibular Saliva Secreted in Response to Methoxamine and Pilocarphie, J Dent Res 66:745-750.[Abstract/Free Full Text]
  • Abe, K.; Yoneda, K.; Fujita, R.; Yokota, Y.; and Dawes, C. (1980): The Effects of Epinephrine, Norepinephrine, and Phenylephrine on the Types of Proteins Secreted by Rat Salivary Glands, J Dent Res 59:1627-1634.[Abstract/Free Full Text]
  • Abe, S. (1985): Sex Differences of the {alpha}1-Adrenoceptor Functions with Aging in Rat Submandibular Glands, Jpn J Oral Biol 27:909-932 (in Japanese).
  • Barka, T. and Van der Noen, H. (1974): Adenylate Cyclase Activity in Rat Submandibular Gland During Postnatal Development, Life Sci 14:267-280.[Medline] [Order article via Infotrieve]
  • Baum, B.J.; Kuyatt, B.L.; and Humphreys, S. (1983): Protein Production and Processing in Young Adult and Aged Rat Submandibular Gland Cells in vitro, Mech Aging Dev 23:123-136.
  • Bunge, R.; Johnson, M.; and Ross, C.D. (1978): Nature and Nurture in Development of the Autonomic Neurons, Science 199:1409-1416.[Abstract/Free Full Text]
  • Bylund, D.B.; Martinez, J.R.; Camden, J.; and Jones, S.B. (1982): Autonomic Receptors in the Developing Submandibular Glands of Neonatal Rats, Arch Oral Biol 27:945-950.[CrossRef][Medline] [Order article via Infotrieve]
  • Champlain, J.; DE Malmfors, T.; Olson, L.; and Sachs, C. (1970): Ontogenesis of Peripheral Adrenergic Neurons in the Rat: Pre- and Postnatal Observations, Acta Physiol Scand 80:276-288.[Medline] [Order article via Infotrieve]
  • Cutler, L.S. and Chaudhry, A.P. (1974): Cytodifferentiation of the Acinar Cells of the Rat Submandibular Gland, Dev Biol 41:31-41.[Medline] [Order article via Infotrieve]
  • Cutler, L.S. and Chaudhry, A.P. (1975): Cytodifferentiation of Striated Duct Cells and Secretory Cells of the Convoluted Granular Tubules of the Rat Submandibular Gland, Am J Anat 143:201-217.[CrossRef][Medline] [Order article via Infotrieve]
  • Cutler, L.S.; Christian, C.P.; and Bottaro, B. (1981): Development of Stimulus-Secretion Coupling in Salivary Glands, Mech Cell Biol 23:531-545.
  • Denny, P.C. and Cope, P. (1977): Age-specific Differentiations During the Development of the Rat Submandibular Gland, Differentiation 8:105-111.
  • Fukuda, M. (1967): Histochemical Studies on the Rat Submaxillary Gland During Post-natal Development, Histochemistry 8:342-354.
  • Grand, R.J.; Chong, D.A.; and Ryan, S.J. (1975): Postnatal Development of Adenylate Cyclase in Rat Salivary Glands: Patterns of Hormonal Sensitivity, Am J Physiol 228:608-612.[Abstract/Free Full Text]
  • Gresik, E.W. (1980): Postnatal Developmental Changes in Submandibular Glands of Rats and Mice, J Histochem Cytochem 28:860-870.[Abstract]
  • Heukeshoven, J. and Dernick, R. (1985): Simplified Method for Silver Staining of Proteins in Polyacrylamide Gels and the Mechanism of Silver Staining, Electrophoresis 6:103-112.[CrossRef]
  • Ikeno, K.; Kuzuya, H.; and Ishiguro, I. (1984): Developmental Changes of Trypsin-like Protease Activity in the Submandibular Glands of Male and Female Young Growing Rats, Arch Oral Biol 29:669-674.[Medline] [Order article via Infotrieve]
  • Inanaga, T.; Habu, T.; Tanaka, E.; Taniguchi, T.; Nish-Iura, T.; Ishibashi, K.; Naruse, S.; and Abe, K. (1988): Age Changes in Secretory Function of Male and Female Rat Parotid Glands in Response to Methoxamine and Pilocarpine, J Dent Res 67:565-573.[Abstract/Free Full Text]
  • Ito, H.; Baum, B.J.; and Roth, G.S. (1981): β-adrenergic Regulation of Rat Parotid Gland Exocrine Protein Secretion During Aging, Mech Aging Dev 15:177-188.
  • Jacoby, F. and Leeson, C.R. (1959): The Postnatal Development of the Rat Submaxillary Gland, J Anat 93:201-216.[Medline] [Order article via Infotrieve]
  • Kamogashira, K.; Yanabu, M.; Ichikawa, K.; Itoh, T.; Matsumoto, M.; Ishibashi, K.; and Abe, K. (1988): The Effects of Upper Incisor Separation on the Submandibular and Sublingual Glands of Rats, J Dent Res 67:602-610.[Abstract/Free Full Text]
  • Kim, S.K.; Han, S.S.; and Nasjleti, C.E. (1970): The Fine Structure of Secretory Granules in Submandibular Glands of the Rat During Early Postnatal Development, Anat Rec 168:463-476.[Medline] [Order article via Infotrieve]
  • Laemmli, U.K. (1970): Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature 227:680-685.[CrossRef][Medline] [Order article via Infotrieve]
  • Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
  • Martinez, J.R. and Camden, J. (1983): Volume and Composition of Pilocarpine- and Isoproterenol-stimulated Submandibular Saliva of Early Postnatal Rats, J Dent Res 62:543-547.[Abstract/Free Full Text]
  • Matthews, R.W. (1974): The Effects of Autonomic Stimulation Upon the Rat Submandibular Gland, Arch Oral Biol 19:989-994.[CrossRef][Medline] [Order article via Infotrieve]
  • Menaker, L. and Miller, S.A. (1973): Changes in Nucleic Acid and Protein Content During Development of the Rat Submandibular Gland, Arch Oral Biol 18:1325-1336.[Medline] [Order article via Infotrieve]
  • Muenzer, J.; Bildstein, C.; Gleason, M.; and Carlson, D.M. (1979a): Purification of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats, J Biol Chem 254:5623-5628.[Abstract/Free Full Text]
  • Muenzer, J.; Bildstein, C.; Gleason, M.; and Carlson, D.M. (1979b): Properties of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats, J Biol Chem 254:5629-5634.[Free Full Text]
  • Ørstavik, T.B.; Nustad, K.; and Brandtzaeg, P. (1977): A Biochemical and Immunohistochemical Study of Kallikrein in Normal and Isoproterenol-stimulated Rat Salivary Glands During Postnatal Development, Arch Oral Biol 22:495-502.[Medline] [Order article via Infotrieve]
  • Sreebny, L.M.; Meyer, J.; Bachem, E.; and Weinmann, J.P. (1955): Postnatal Changes in Proteolytic Activity and in the Morphology of the Submaxillary Gland in Male and Female Albino Rats, Growth 19:57-74.[Medline] [Order article via Infotrieve]
  • Srinivasan, R. and Chang, W.W.L. (1975): The Development of the Granular Convoluted Duct in the Rat Submandibular Gland, Anat Rec 182:29-40.[CrossRef][Medline] [Order article via Infotrieve]
  • Towbin, H.; Staehelin, T.; and Gordon, J. (1979): Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications, Proc Natl Acad Sci USA 76:4350-4354.[Abstract/Free Full Text]
  • Yamashina, S. and Barka, T. (1974): Peroxidase Activity in the Developing Rat Submandibular Gland, Lab Invest 31:82-89.
  • Yoshida, Y.; Sprecher, R.L.; Schneyer, C.A.; and Schneyer, L.H. (1967): Role of β-Receptors in Sympathetic Regulation of Electrolytes in Rat Submaxillary Saliva, Proc Soc Exp Biol Med 126:912-916.[CrossRef]
  • Yu, J.H.; Mark, M.R.; and Redman, R.S. (1987): Effect of Clonidine on Secretion of Fluid and Ions by the Parotid and Submandibular Glands of the Rat, Arch Oral Biol 32:27-33.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 69, No. 1, 60-66 (1990)
DOI: 10.1177/00220345900690011001
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