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Journal of Dental Research
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Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat

D.F. Turner

Department of Oral Biology, The University of Michigan Dental School, Ann Arbor, Michigan 48109-1078

C.F. Marfurt

Department of Anatomy, Indiana University School of Medicine, Northwest Center for Medical Education, Gary, Indiana 46408

C. Sattelberg

Department of Oral Biology, The University of Michigan Dental School, Ann Arbor, Michigan 48109-1078

Vascular injection of the macromolecular tracer, horseradish peroxidase (HRP), was used to study the permeability of the odontoblast cell layer in developing and mature rat molar teeth, and to investigate the effect of cavity preparations on the permeability of this epithelioid cell layer in adult animals. HRP injected into the vascular system of normal animals 28 days of age and older was localized histochemically (from 5 to 90 min after injection) throughout the extracellular spaces of the maxillary dental pulps; however, the tracer did not penetrate beyond the tight junctions at the apical region of the odontoblast cell layer, and was absent from the predentin and dentin. In contrast, HRP injected into very young neonatal animals (e.g., day 3) resulted in free passage of HRP between odontoblasts and into the overlying predentin and dentin. When Class V cavities had been prepared in adult maxillary molars after HRP was injected into the blood stream, HRP reaction product penetrated the predentin and dentin immediately beneath the cavity preparation; however, adjacent, un-traumatized areas of predentin and dentin in the operated teeth were devoid of reaction product. These results provide evidence that: (1) a physiological barrier develops between the distal segments of odontoblast cell bodies in normal rat molar teeth between days 15 and 28 of postnatal life, and this barrier prevents the passage of macromolecules from the pulp into the predentin and dentin; and (2) this barrier is perturbed following routine restorative procedures in adult animals.

REFERENCES

  • Adams, J.C. (1977): Technical Considerations on the Use of Horseradish Pcroxidasc as a Neuronal Marker, Neuroscience 2:141-145.[CrossRef][Medline] [Order article via Infotrieve]
  • Arwill, T. (1967): Studies on the Ultrastructure of Dental Tissues, Odont Revy 18:191-208.
  • Arwill, T. (1968): The Ultrastructurc of the Pulpo-Dentinal Border Zone. In: Dentin and Pulp, N.B.B. Symons, Ed., Baltimorc: Williams and Wilkins Co., pp. 147-167.
  • Bishop, M.A. (1985): Evidence for Tight Junctions Between Odontoblasts in the Rat Incisor, Cell Tissue Res 239:137-140.[Medline] [Order article via Infotrieve]
  • Bishop, M.A. (1987): An Investigation of Pulp Capillaries and Tight Junctions Between Odontoblasts in Cats, Anat Embryol 177:131-138.[Medline] [Order article via Infotrieve]
  • Brannstrom, M. (1963): Dentin Sensitivity and Aspiration of Odontoblasts, J Am Dent Assoc 66:366-370.[Medline] [Order article via Infotrieve]
  • Brannstrom, M. and Astrom, A. (1964): A Study on the Mechanism of Pain Elicited from the Dentin, J Dent Res 43:619-625.[Free Full Text]
  • Byers, M.R. (1977): Fine Structure of Trigeminal Receptors in Rat Teeth. In: Pain in the Trigeminal Region, D.J. Anderson and B. Matthews, Eds., North Holland: Elsevier, pp. 13-24.
  • Byers, M.R. (1979): Large and Small Trigeminal Nerve Endings and Their Associations With Odontoblasts in Rat Molar Dentin and Pulp, Adv Pain Res Ther 3:265-270.
  • Byers, M.R. (1984): Dentttl Sensory Receptors, Int Rev Neurobiol 25:39-93.[Medline] [Order article via Infotrieve]
  • Carson, K.A. and Mesulam, M.-M. (1982): Electron Microscopic Demonstration of Neural Connections Using Horseradish Peroxidase: a Comparison of the Tetramethylbenzidine Procedure with Seven Other Histochemical Methods, J Histochem Cytochem 30:425-435.[Abstract]
  • Calle, H. (1985): Intercellular Junctions Between Human Odontoblasts, Acta Anat 122:138-144.[Medline] [Order article via Infotrieve]
  • Dermietzel, R.; Meller, K.; Tetzlaff, W.; and Waelsh, M. (1977): In vivo and in vitro Formation of the Junctional Complex in Choroid Epithclium. A Frcczc-ctching Study, Cell Tissue Res 181:427-441.[Medline] [Order article via Infotrieve]
  • Eda, S. and Saito, T. (1978): Electron Microscopy of Cells Displaced into the Dentinal Tubules due to Dry Cavity Preparation, J Oral Puthol 7:326-335.
  • Farquhar, M.G. and Palade, G.E. (1963): Junctional Complexes in Various Epithclia, J Cell Biol 17:375-408.[Abstract/Free Full Text]
  • Frank, R.M. (1968): Ultrastructural Relationship Between the Odontoblast, its Process and the Nerve Fibre. In: Dentine and Pulp. Their Structure and Reactions, N.B.B. Symons, Ed., Baltimore: Williams and Wilkins Co., pp. 115-146.
  • Garant, P.R.; Szabo, G.; and Nalbandian, J. (1968): The Fine Structure of the Mouse Odontoblast, Arch Oral Biol 13:857-876.[CrossRef][Medline] [Order article via Infotrieve]
  • Graham, R.C. and Karnovsky, M.J. (1966): The Early Stages of Absorption of Injected Horseradish Peroxidase in the Proximal Tubule of Mouse Kidney: Ultrastructural Cytochemistry by a New Technique, J Histochem Cytochem 14:291-302.[Abstract]
  • Goodenough, D.A. and Revel, J.P. (1970): A Fine Structural Analysis of Intercellular Junctions in the Mouse Liver, J Cell Biol 45:272-290.[Abstract/Free Full Text]
  • Grynszpan-Wynograd, O. and Nicolas, G. (1980): Intercellular Junctions in the Adrenal Medulla: A Comparative Freeze Fracture Study, Tissue Cell 12:661-672.[CrossRef][Medline] [Order article via Infotrieve]
  • Hirvonen, T.J. and Narhi, M. (1986): The Effect of Dentinal Stimulation on Pulp Nerve Function and Pulp Morphology in the Dog, J Dent Res 65:1290-1293.[Abstract/Free Full Text]
  • Holland, G.R. (1975): Membrane Junctions on Cat Odontoblasts, Arch Oral Biol 20:551-552.[Medline] [Order article via Infotrieve]
  • Holland, G.R. (1976): Lanthanum Hydroxide Labelling of Gap Junctions in the Odontoblast Layer, Anat Rec 186:121-126.
  • Holland, G.R. (1980): Non-myclinated Nerve Fibers and Their Terminals in the Subodontoblast Plexus of the Feline Dental Pulp, J Anat 130:457-467.[Medline] [Order article via Infotrieve]
  • Holland, G.R. (1987): The Effect of Nerve Section on the Incidence and Distribution of Gap Junctions in the Odontoblast Layer of the Cat, Anat Rec 218:458-465.[CrossRef][Medline] [Order article via Infotrieve]
  • Iguchi, Y.; Yamamura, T.; Ichikawa, T.; Hashimoto, S.; Horiuchi, T.; and Shimono, M. (1984): Intcrccllular Junctions in Odontoblasts of the Rat Incisor Studied with Freeze-Fracture, Arch Oral Biol 29:487-497.[CrossRef][Medline] [Order article via Infotrieve]
  • Jessen, H. (1967): The Ultrastructure of Odontoblasts in Pcrfusion Fixed, Demineralized Incisors of Adult Rats, Acta Odontol Scand 25:491-523.[Medline] [Order article via Infotrieve]
  • Johnson, G.; Olgart, S.; and Brannstrom, M. (1973): Outward Fluid Flow in Dentin Under a Physiologic Pressure Gradient: Experiments in vitro, Oral Surg Oral Med Oral Pathol 35:238-248.[Medline] [Order article via Infotrieve]
  • Koling, A.; Rask-Andersen, H.; and Bagger-Sjoback, D. (1981): Membrane Junctions on Odontoblasts. A Freeze-Fracture Study, Acta Odontol Scand 39:355-360.
  • Koling, A. and Rask-Andersen, H. (1984a): Membrane Junctions Between Odontoblasts and Associated Cells. A Freeze-Fracture Study of the Human Odontoblastic Cell Layer with Special Reference to its Nerve Supply, Acta Odontol Scand 42:13-22.[Medline] [Order article via Infotrieve]
  • Koling, A. and Rask-Andersen, H. (1984b): Membrane Structures in the Pulp Dentin Border Zone. A Frceze-fracture Study of Demineralized Human Teeth, Acta Odontol Scand 138:355-360.
  • Marfurt, C.F. and Turner, D.F. (1983): Sensory Nerve Endings in the Rat Orofacial Region Labeled by the Anterograde and Transganglionic Transport of Horseradish Peroxidase: a New Method for Tracing Peripheral Nerve Fibers, Brain Res 261:106-117.
  • Marfurt, C.F.; Turner, D.F.; and Adams, C.E. (1988): Stabilization of Tetramethylbenzidine (TMB) Reaction Product at the Electron Microscopic Level by Ammonium Molybdate, J Neurosci Meths 25:215-223.
  • Matthews, B. and Holland, G.R. (1975): Coupling Between Nerves in Teeth, Brain Res 98:354-358.[CrossRef][Medline] [Order article via Infotrieve]
  • Mesulam, M.M. (1978): Tetramethyl Benzidine for Horseradish Peroxidase Neurohistochemistry: a Non-carcinogenic Blue Reaction Product with Superior Sensitivity for Visualizing Ncural Afferents and Efferents, J Histochem Cytochem 26:106-117.[Abstract]
  • Nagai, N. and Frank, R.M. (1974): Electron Microscopic Autoradiography of Ca45 During Dentinogencsis, Cell Tissue Res 155:513-523.[Medline] [Order article via Infotrieve]
  • Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and Okabe, A. (1983a): The Effects of Acid Etching on the in vivo Permeability of Dentine in the Dog, Arch Oral Biol 29:853-858.
  • Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and Okabe, A. (1983b): Progressive Decrease in Dentine Permeability Following Cavity Preparation, Arch Oral Biol 28:853-858.[CrossRef][Medline] [Order article via Infotrieve]
  • Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and O'Meara, J.A. (1984): The Effect on Dentine Permeability of Time Following Cavity Preparation in Dogs, Arch Oral Biol 29:65-68.[CrossRef][Medline] [Order article via Infotrieve]
  • Pashley, D.H.; Livingston, M.J.; and Greenhill, J.D. (1978): Regional Resistance to Fluid Flow in Human Dentine in vitro, Arch Oral Biol 23:807-810.[CrossRef][Medline] [Order article via Infotrieve]
  • Pashley, D.H.: Nelson, R.; and Williams, E.C. (1981): Dentine Hydraulic Conductance: Changes Produced by Red Blood Cells, J Dent Res 60:1779-1802.
  • Reith, E.J. (1968): Collagen Formation in Developing Molar Teeth of Rats, J Ultrastruct Res 21:383-414.[CrossRef]
  • Sakumoto, T.; Nagai, T.; Kimura, H.; and Maeda, T. (1980): Electron Microscopic Visualization of Tetramethyl-Benzidine Reaction Product on Horseradish Peroxidase Neurohistochcmistry, Cell Mol Biol 26:211-216.[Medline] [Order article via Infotrieve]
  • Sasaki, T.; Ishida, I.; and Higashi, S. (1982a): Ultrastructure and Cytochemistry of Old Odontoblasts in Rat Incisors, J Electron Microsc 31:378-388.[Abstract/Free Full Text]
  • Sasaki, T.; Nakagawa, K.; and Higashi, S. (1982b): Ultrastructurc of Odontoblasts in Kitten Tooth Germs as Rcvcalcd by Freeze-fracture, Arch Oral Biol 27:897-904.[CrossRef][Medline] [Order article via Infotrieve]
  • Sattelberg, C.F. and Turner, D.F. (1984): Anatomical Evidence for the Existence of Zonula Occludens Between Pulpal Odontoblasts, J Dent Res 63:225, Abst. No. 494.
  • Sattelberg, C. and Turner, D.F. (1985): Permeability of the Pulpal Odontoblast Cell Layer Following Cavity Preparation, J Dent Res 64:240, Abst. No. 588.
  • Stewart, J.M. (1965): The Immediate Response of Odontoblasts to Injury, Odont Tidskr 73:417-423.
  • Takuma, S. and Nagai, N. (1971): Ultrastructure of Rat Odontoblasts in Various Stages of Their Development and Maturation, Arch Oral Biol 16:993-1011.[CrossRef][Medline] [Order article via Infotrieve]
  • Tanaka, T. (1980): The Origin and Localization of Dental Fluid in Developing Rat Molar Teeth Studied With Lanthanum as a Tracer, Arch Oral Biol 25:153-162.[Medline] [Order article via Infotrieve]
  • Tice, L.W.; Carter, R.L.; and Cahil, M.C. (1977): Tracer and Freeze-fracture Observations on Developing Tight Junctions in Fetal Rat Thvroid, Tissue Cell 9:395-418.[CrossRef][Medline] [Order article via Infotrieve]
  • Turner, D.F. (1982): Ultrastructure of the Primate Dental Pulp Organ, Anat Rec 202:193A.

Journal of Dental Research, Vol. 68, No. 8, 1262-1268 (1989)
DOI: 10.1177/00220345890680081001
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