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Journal of Dental Research
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Associations between Salivary Levels of Streptococcus mutans, Streptococcus sobrinus, Lactobacilli, and Caries Experience in Kenyan Adolescents

D. Beighton

Dental Research Unit, Royal College of Surgeons of England, Downe, Kent, United Kingdom BR6 7JJ

F. Manji

Oral Health Research Unit, KEMRI, Nairobi, Kenya

V. Baelum

Royal Dental School, Aarhus, Denmark

O. Fejerskov

Royal Dental School, Aarhus, Denmark

N.W. Johnson

Dental Research Unit, Royal College of Surgeons of England, Downe, Kent, United Kingdom BR6 7JJ, MRC, Dental Research Unit, Periodontal Diseases Programme, London Hospital Medical College, London, England

J.M.A. Wilton

MRC, Dental Research Unit, Periodontal Diseases Programme, London Hospital Medical College, London, England

Salivary levels of mutans streptococci (S. mutans and S. sobrinus) and lactobacilli were determined in a random sample of rural Kenyans between 15 and 19 years of age (n = 149). It is possible for the natural history of dental caries in this population to be studied since it is characterized by a limited access to conventional dental treatment. Using a short set of biochemical tests, we identified from seven to ten presumptive mutans streptococcus colonies-cultured from the saliva of each individual-to differentiate between S. mutans and S. sobrinus. No colonies resembling S. rattus (S. mutans serotype b) were isolated. Lactobacilli were identified as Gram-positive, catalase-negative rods. The mean D1-4MFS and D3-4MFS were 7.03 ± 6.43 and 1.46 ± 3.44, respectively. The mean mutans streptococcus and lactobacillus levels were 8. 7 x 104 and 6. 7 x 104, respectively. The salivary mutans streptococcus and lactobacillus levels were significantly correlated (p<0.01). Of the subjects, 64% harbored only S. mutans, 4% only S. sobrinus, 30% both species, and 2% neither. Lactobacilli were ubiquitous. The caries experience of the group was significantly (p<0.001) correlated with both the total salivary level of mutans streptococci and the salivary S. mutans levels, but not with the salivary S. sobrinus level.

REFERENCES

  • Bader, J.D.; Graves, R.C.; Disney, J.A.; Bohannan, H.M.; Stamm, J.W.; Abernathy, J.R.; and Lindahl, R.L. (1986): Identifying Children who will Experience High Caries Increments, Community Dent Oral Epidemiol 14:198-201.[CrossRef][Medline] [Order article via Infotrieve]
  • Baelum, V.; Fejerskov, O.; and Manji, F. (1989): Periodontal Diseases in Adult Kenyans, J Clin Periodontol (in press).
  • Beighton, D. (1985): Streptococcus mutans and other Streptococci from the Oral Cavity, Soc Appl Bacteriol Tech Series 21:177-190.
  • Beighton, D. (1986): A Simplified Procedure for Estimating the Level of Streptococcus mutans in the Mouth, Br Dent J 160:329-330.[Medline] [Order article via Infotrieve]
  • Beighton, D. and Hayday, H. (1984): The Establishment of the Bacterium Streptococcus mutans in Dental Plaque and the Induction of Dental Caries in Macaque Monkeys (Macaca fascicularis) Fed a Diet Containing Cooked-Wheat Flour, Arch Oral Biol 29:369-372.[Medline] [Order article via Infotrieve]
  • Beighton, D.; Hayday, H.; Russell, R.R.B; and Whiley, R.A. (1984): Streptococcus macacae sp. nov. from Dental Plaque of Monkeys (Macaca fascicularis), Int J System Bacteriol 34:332-335.[Abstract/Free Full Text]
  • Beighton, D.; Hayday, H.; and Walker, J. (1982): The Acquisition of Streptococcus mutans by Infant Monkeys (Macaca fascicularis) and its Relationship to the Initiation of Dental Caries, J Gen Microbiol 128:1881-1892.[Abstract/Free Full Text]
  • Beighton, D.; Rippon, H.R.; and Thomas, H.E.C. (1987): The Distribution of Streptococcus mutans Serotypes and Dental Caries Experience in a Group of 5-8-ycar-old English Schoolchildren, Br Dent J 162:103-106.[CrossRef][Medline] [Order article via Infotrieve]
  • Beighton, D.; Russell, R.R.B.; and Hayday, H. (1981): The Isolation and Characterisation of Streptococcus mutans Serotype h from Dental Plaque of Monkeys (Macaca fascicularis), J Gen Microbiol 124:271-279.[Abstract/Free Full Text]
  • Bratthall, D. (1970): Demonstration of Five Serological Groups of Streptococcal Strains Resembling Streptococcus mutans, Odontol Revy 21:143-152.[Medline] [Order article via Infotrieve]
  • Bratthall, D.; Serinirach, R.; Carlsson, P.; and Lek-Fuangfu, S. (1986): Streptococcus mutans and Caries Prevalence in Urban and Rural Schoolchildren in Thailand, Community Dent Oral Epidemiol 14:274-276.[Medline] [Order article via Infotrieve]
  • Carlsson, P.; Gandour, LA; Olsson, B.; Rickards-Son, B.; and Abbas, K. (1987): High Prevalence of Mutans Streptococci in a Population with Extremely Low Prevalence of Dental Caries, Oral Microbiol Immunol 2:121-124.[Medline] [Order article via Infotrieve]
  • Carlsson, P.; Olsson, B.; and Bratthall, D. (1985): The Relationship between the Bacterium Streptococcus mutans in Saliva and Dental Caries in Children in Mozambique, Arch Oral Biol 29:385-393.
  • Clarke, J.K. (1924): On the Bacterial Factor in the Aetiology of Dental Caries, Br J Exp Pathol 5:141-147.
  • Crossner, C.G. (1981): Salivary Lactobacillus Counts in the Prediction of Caries Activity, Community Dent Oral Epidemiol 9:182-190.[Medline] [Order article via Infotrieve]
  • De Stoppelaar, J.D.; Van Houte, I.; and Backer Dirks, O. (1970): The Effect of Carbohydrate Restriction on the Presence of Streptococcus mutans, Streptococcus sanguis and lodophilic Polysaccharide-producing Bacteria in Human Dental Plaque, Caries Res 4:114-123.[Medline] [Order article via Infotrieve]
  • El Tayeb, Y.; Bratthall, D.; and Carlsson, P. (1985): Dental Caries and Streptococcus mutans in Sudanese Schoolchildren, Odontostomatol Trop 8:77-80.[Medline] [Order article via Infotrieve]
  • Emilson, C.-G. (1983): Prevalence of Streptococcus mutans with Different Colonial Morphologies in Human Plaque and Saliva, Scand J Dent Res 91:26-32.[Medline] [Order article via Infotrieve]
  • Emilson, C.-G. and Krasse, B. (1985): Support for and Implications of the Specific Plaque Hypothesis, Scand J Dent Res 93:96-104.[Medline] [Order article via Infotrieve]
  • Fitzgerald, R.J. (1968): Dental Caries Research in Gnotobiotic Animals, Caries Res 2:139-146.[Medline] [Order article via Infotrieve]
  • Gold, O.G.; Jordan, H.V.; and Van Houte, J.A. (1973): A Selective Medium for Streptococcus mutans, Arch Oral Biol 18:1357-1364.[CrossRef][Medline] [Order article via Infotrieve]
  • Hamada, S. and Slade, H.H. (1980): Biology, Immunology and Cariogenicity of Streptococcus mutans, Microbiol Rev 44:331-384.[Free Full Text]
  • Holbrook, W.P. and Beighton, D. (1986): Streptococcus mutans in Saliva and Distribution of Serotypes among 9-year-old Icelandic Children, Scand J Dent Res 95:37-42.
  • Huis In't Veld, J.H.J.; Van Palenstein-Helderman, W.; and Backer-Dirks, O. (1979): Streptococcus mutans and Dental Caries in Humans: A Bacteriological and Immunological Study, Antonie van Leeuwenhoek 45:25-33.[CrossRef][Medline] [Order article via Infotrieve]
  • Kingman, A.; Little, W.; Gomez, I.; Heifetz, S.B.; Dris-Coll, W.S.; Sheats, R.; and Supan, P. (1988): Salivary Levels of Streptococcus mutans and Lactobacilli and Dental Caries Experience in a US Adolescent Population, Community Dent Oral Epidemiol 16:98-103.[CrossRef][Medline] [Order article via Infotrieve]
  • Kloch, B. and Krasse, B. (1977): Microbial and Salivary Conditions in 9- to 12-year-old Children, Scand J Dent Res 85:56-63.[Medline] [Order article via Infotrieve]
  • Kohler, B. and Bjarnason, S. (1987): Mutans Streptococci, Lactobacilli and Caries Prevalence in 11- and 12-year-old Icelandic Children, Community Dent Oral Epidemiol 15:332-335.[CrossRef][Medline] [Order article via Infotrieve]
  • Kristoffersson, K.; Grondahl, H.-G.; and Bratthall, D. (1985): The More Streptococcus mutans, the More Caries on the Approximal Surfaces, J Dent Res 64:58-61.[Free Full Text]
  • Loesche, W.J.; Eklund, S.; Earnest, R.; and Burt, B.A. (1984): Longitudinal Investigation of Bacteriology of Human Fissure Decay; Epidemiological Studies in Molars Shortly After Eruption, Infect Immun 46:765-772.[Abstract/Free Full Text]
  • Loesche, W.J. (1986): The Role of Streptococcus mutans in Human Dental Decay, Microbiol Rev 50:353-380.[Free Full Text]
  • Manji, F.; Baelum, V.; and Fejerskov, O. (1988): Tooth Mortality in an Adult Rural Population in Kenya,.J Dent Res 67:496-500.[Abstract/Free Full Text]
  • Manji, F.; Fejerskov, O.; and Baelum, V. (1989): The Natural History of Dental Caries in an Adult Rural Population, Caries Res (in press).
  • Matee, M.I.; Mikx, F.H.M.; Frencken, J.E.F.N.; Truin, G.J.; and Ruiken, H.M.H.M. (1985): Selection of a Micro-method and its Use in the Estimation of Salivary Streptococcus mutans and Lactobacillus Counts in Relation to Dental Caries in Tanzanian Children, Caries Res 19:497-506.[Medline] [Order article via Infotrieve]
  • Perch, B.; Kjems, E.; and Ravn, D. (1974): Biochemical and Serological Properties of Streptococcus mutans from Various Animal and Human Sources, Acta Pathol Microbiol Immunnl Scand Sect B 82:357-370.
  • Rogosa, M.; Mitchell, J.A.; and Wiseman, R. (1951): A Selective Medium for the Isolation and Enumeration of Oral Lactobacilli, J Dent Res 30:682-689.[Free Full Text]
  • Schaeken, M.J.M.; De Jong, M.H.; Franken, H.C.M.; and Van der Hoeven, J.S. (1984): Effect of Chlorhexidine and Iodine on the Composition of the Human Dental Plaque Flora, Caries Res 18:401-407.[Medline] [Order article via Infotrieve]
  • Stecksen-Blicks, C. (1985): Salivary Counts of Lactobacilli and Streptococcus mutans in Caries Prediction, Scand J Dent Res 93:204-212.[Medline] [Order article via Infotrieve]
  • Thomson, L.A.; Little, W.A.; Bowen, W.H.; Sierra, L.I.; Aguirrer, M.; and Gillespie, G. (1980): Prevalence of Streptococcus mutans Serotypes, Actinomyces, and Other Bacteria in the Plaque of Children, J Dent Res 59:1581-1589.[Abstract/Free Full Text]
  • Togelius, J.; Kristoffersson, K.; Andersson, H.; and Bratthall, D. (1984): Streptococcus mutans in Saliva: Intra-individual Variations and Relation to Number of Colonized Sites, Acta Odontol Scand 42:157-163.[CrossRef][Medline] [Order article via Infotrieve]
  • Van Houte, J. and Green, D.B. (1974): Relationship between the Concentration of Bacteria in Saliva and the Colonization of Teeth in Humans, Infect Immun 9:624-630.[Abstract/Free Full Text]
  • Van Palenstein-Helderman, M.; Ijsseldijk, M.; and Huis In't Veld, J.H.J. (1983): A Selective Medium for the Two Major Subgroups of the Bacterium Streptococcus mutans Isolated from Human Dental Plaque and Saliva, Arch Oral Biol 28:599-603.[CrossRef][Medline] [Order article via Infotrieve]
  • Van Steenbergen, W.M.; Kusin, J.A.; and Onchere, S.R. (1984): Household Activities and Dietary Patterns. In: Maternal and Child Health in Rural Kenya, J.K. Van Ginnekin and A.S. Muller, Eds., London: Croom Helm, pp. 31-42.

Journal of Dental Research, Vol. 68, No. 8, 1242-1246 (1989)
DOI: 10.1177/00220345890680080601
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W.H. van Palenstein Helderman, M.I.N. Matee, J.S. van der Hoeven, and F.H.M. Mikx
Cariogenicity Depends More on Diet than the Prevailing Mutans Streptococcal Species
Journal of Dental Research, January 1, 1996; 75(1): 535 - 545.
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