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Journal of Dental Research
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Possible Function of Matrix Proteins in Fluoride Incorporation into Enamel Mineral during Porcine Amelogenesis

T. Aoba

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

J. Collins

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

E.C. Moreno

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

The present study was undertaken to elucidate the mechanism of fluoride incorporation into secretory enamel mineral, with porcine enamel used as a model. Although the fluoride content in the enamel varied greatly among the animals, we observed that the fluoride-to-calcium ratio in the enamel tissue was maximal at the beginning of the secretory stage; the F/Ca ratio decreased (and leveled off) with the advancement of mineralization. In vitro work showed that some of the fluoride in the secretory enamel tissue was removed with the extraction of organic matter, mostly amelogenins. Furthermore, coating hydroxyapatite crystals with enamel matrix proteins resulted in a retardation of fluoride incorporation into the crystals when exposed to fluoride solutions, as a result of an inhibition of apatite reprecipitation. We also confirmed that the growth kinetics of fluoridated apatite onto HA seeds decreased with increasing coverage of the seed surface with the enamel proteins_ All the results of the present study strongly suggest that the fluoride incorporation into enamel mineral during the secretory stage may be regulated by the kinetics of mineralization, which is highly dependent on the driving force for precipitation and the presence of proteinaceous inhibitors, mainly amelogenins.

REFERENCES

  • Ahlberg, M.; Bawden, J.W.; Hammarström, L.E.; and Hellborg, R. (1975): Proton Activation Analysis on Fluorine and Nitrogen Content of Developing Dental Enamel, Odont Revy 26:267-280.
  • Aoba, T.; Fukae, M.; Tanabe, T.; Shimizu, M.; and Moreno, E.C. (1987a): Selective Adsorption of Porcine Amelogenins onto Hydroxyapatite and Their Inhibitory Activity on Seeded Crystal Growth of Hydroxyapatite, Calcif Tissue Int 41:281-289.[Medline] [Order article via Infotrieve]
  • Aoba, T. and Moreno, E.C. (1984): Hydroxyapatite Preparation and Crystal Growth on Hydroxyapatite Seeds, Dent Res 63:874-880.
  • Aoba, T. and Moreno, E.C. (1987): The Enamel Fluid in the Early Secretory Stage of Porcine Amelogenesis. Chemical Composition and Saturation with Respect to Enamel Mineral, Calcif Tissue Int 41:86-94.[Medline] [Order article via Infotrieve]
  • Aoba, T.; Tanabe, T.; and Moreno, E.C. (1987b): Proteins in the Enamel Fluid of Immature Porcine Teeth, J Dent Res 66:1721-1726.[Abstract/Free Full Text]
  • Aoba, T.; Tanabe, T.; and Moreno, E.C. (1987c): Function of Amelogenins in Porcine Enamel Mineralization during the Secretory Stage of Amelogenesis, Adv Dent Res 1:252-260.[Abstract/Free Full Text]
  • Bawden, J.W. and Crenshaw, M.A. (1984): Effects of Inhibition of Net Calcium Uptake on Net Fluoride Uptake in Developing Rat Molars, J Dent Res 63:642-645.[Abstract/Free Full Text]
  • Bawden, J.W. and Deaton, T.G. (1981): In-vitro Study of the Cellular Control of 18F Uptake in the Enamel of Developing Rat Molar Teeth, Arch Oral Biol 26:487-490.[CrossRef][Medline] [Order article via Infotrieve]
  • Bawden, J.W.; Deaton, T.G.; and Crenshaw, M.A. (1982): Role of the Enamel Organ in Limiting Fluoride Uptake During the Maturation Phase of Enamel Development, J Dent Res 61:506-509.[Abstract/Free Full Text]
  • Bawden, J.W.; Deaton, T.G.; and Crenshaw, M.A. (1987): Diffusion of Fluoride Through the Rat Enamel Organ in vitro, J Dent Res 66:1360-1363.[Abstract/Free Full Text]
  • Crenshaw, M.A. and Bawden, J.W. (1981): Fluoride-binding by Organic Matrix from Early and Late Developing Bovine Fetal Enamel Determined by Flowrate Dialysis, Arch Oral Biol 26:473-476.[CrossRef][Medline] [Order article via Infotrieve]
  • Crenshaw, M.A.; Wennberg, A.; and Bawden, J.W. (1978): Fluoride-binding by the Organic Matrix of Developing Bovine Enamel, Arch Oral Biol 23:285-287.[CrossRef][Medline] [Order article via Infotrieve]
  • Deutsch, D. and Gedalia, I. (1982): Fluoride Concentration in Human Fetal Enamel, Caries Res 16:428-432.[Medline] [Order article via Infotrieve]
  • Fridell, R.A.; Lussi, A.; Crenshaw, M.A.; and Bawden, J.W. (1988): The in vitro Uptake of Fluoride by Secretory and Maturation Stage Bovine Enamel, J Dent Res 67:487-490.[Abstract/Free Full Text]
  • Fukae, M. and Shimizu, M. (1974): Studies of the Proteins of Developing Bovine Enamel, Arch Oral Biol 19:381-386.[CrossRef][Medline] [Order article via Infotrieve]
  • Fukae, M. and Shimizu, M. (1985): Amino Acid Sequence of a Protein from Developing Porcine Enamel, Jpn J Oral Biol (Suppl) 25:29.
  • Fukae, M. and Tanabe, T. (1987): Nonamelogenin Components of Porcine Enamel in the Protein Fraction Free from the Enamel Crystals, Calcif Tissue Int 40:286-293.[Medline] [Order article via Infotrieve]
  • Hammarström, L.E. (1971): Distribution in Developing Rat Enamel of Simultaneously Injected Fluoride and Calcium, Scand J Dent Res 79:369-376.[Medline] [Order article via Infotrieve]
  • Laemmli, U.K. (1970): Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature 227:680-685.[CrossRef][Medline] [Order article via Infotrieve]
  • Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
  • Lussi, A.; Fridell, R.A.; Crenshaw, M.A.; and Bawden, J.W. (1988): Absence of in vitro Fluoride-binding by the Organic Matrix of Developing Bovine Enamel, Arch Oral Biol 33:531-533.[CrossRef][Medline] [Order article via Infotrieve]
  • Moreno, E.C. and Aoba, T. (1987): Calcium Binding in Enamel Fluid and Driving Force for Enamel Mineralization in the Secretory Stage of Amelogenesis, Adv Dent Res 1:245-251.[Abstract/Free Full Text]
  • Moreno, E.C.; Kresak, M.; and Zahradnik, R.T. (1977a): Physicochemical Aspects of Fluoride-apatite Systems Relevant to the Study of Dental Caries, Caries Res (Suppl 1) 11:142-171.[Medline] [Order article via Infotrieve]
  • Moreno, E.C.; Zahradnik, R.T.; Glazman, A.; and Hwu, R. (1977b): Precipitation of Hydroxyapatite from Dilute Solutions upon Seeding, Calcif Tissue Res 24:47-57.[CrossRef][Medline] [Order article via Infotrieve]
  • Rankine, C.A.N.; Moreno, E.C.; Vogel, G.L.; and Mar-Golis, H.C. (1985): Micro-analytical Determination of pH, Calcium and Phosphate in Plaque Fluid, J Dent Res 64:1275-1280.[Abstract/Free Full Text]
  • Robinson, C.; Kirkham, J.; and Hallsworth, A.S. (1988): Volume Distribution and Concentration of Protein, Mineral and Water in Developing Bovine Enamel, Arch Oral Biol 33:159-162.[CrossRef][Medline] [Order article via Infotrieve]
  • Speirs, R.L. (1975): Fluoride Incorporation into Developing Enamel of Permanent Teeth in the Domestic Pig, Arch Oral Biol 20:877-883.[CrossRef][Medline] [Order article via Infotrieve]
  • Vogel, A.J. (1961): Quantitative Inorganic Analysis, 3rd ed., New York: John Wiley & Sons, p. 810.
  • Weatherell, J.A.; Deutsch, D.; Robinson, C.; and Hallsworth, A.S. (1975a): Assimilation of Fluoride by Enamel Throughout the Life of the Tooth, Caries Res 11 (Suppl 1):85-115.
  • Weatherell, J.A.; Deutsch, D.; Robinson, C.; and Hallsworth, A.S. (1975b): Fluoride Concentrations in Developing Enamel, Nature 256:230-232.[CrossRef][Medline] [Order article via Infotrieve]
  • Whitford, G.M. and Reynolds, K.E. (1979): Plasma and Developing Enamel Fluoride Concentrations During Chronic Acid-base Disturbances, J Dent Res 58:2058-2065.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 68, No. 7, 1162-1168 (1989)
DOI: 10.1177/00220345890680070501
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