Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Wise, G.E.
Right arrow Articles by Fan, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wise, G.E.
Right arrow Articles by Fan, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Changes in the Tartrate-resistant Acid Phosphatase Cell Population in Dental Follicles and Bony Crypts of Rat Molars during Tooth Eruption

G.E. Wise

Department of Anatomy, Texas College of Osteopathic Medicine, 3500 Camp Bowie Boulevard, Fort Worth, Texas 76107

W. Fan

Department of Anatomy, Texas College of Osteopathic Medicine, 3500 Camp Bowie Boulevard, Fort Worth, Texas 76107

It was the aim of this study to determine the cellular changes that occur in the enamel organ, dental follicle, and surrounding bony crypt of the rat molar prior to and during tooth eruption. By use of light microscope histochemistry to detect cells containing tartrate-resistant acid phosphatase (TRAP), it was seen that TRAP-positive mononuclear cells were present in the dental follicle prior to the onset of eruption (e.g., three days postnatal age) and then declined in number during eruption. Concurrently, TRAP-positive osteoclasts were initially present in large numbers on the surface of the bony crypt surrounding the molars (three days postnatal age) and then declined in number as eruption progressed. Electron microscopy confirmed that these were mononuclear cells and osteoclasts. The results suggest that the mononuclear cells are either precursors of the osteoclasts or perhaps release cytokines that affect osteoclast formation or activity.

Staining for alkaline phosphatase (ALP) activity indicated that at an early postnatal age (secretory stage of amelogenesis), ALP was detected only in the stratum intermedium of the enamel organ, whereas at a later age (maturation phase of amelogenesis), it was present only in the ameloblasts. These results, combined with a survey of the literature, strongly suggest that ALP moves from the base of the enamel organ to the enamel itself over a period of time ranging from pre- to post-eruption.

Rat molars are teeth of limited eruption, and the cellular events that occur in eruption appear comparable with what is seen in dog and human dentition, especially in terms of the cellular events seen in the dental follicle prior to and during eruption. Thus, because rat molars are often more amenable to experimental protocol, they may be a suitable choice for answering questions pertaining to tooth eruption in the dog and in humans.

REFERENCES

  • Anderson, T.R. and Toverud, S.U. (1977): Chromatographic Separation of Two Acid Phosphatases from Rat Bone, Calcif Tissue Res 24:187-190.[CrossRef][Medline] [Order article via Infotrieve]
  • Anderson, T.R. and Toverud, S.U. (1979): Purification and Partial Characterization of Two Acid Phosphatases from Rat Bone, Calcif Tissue Int 27:219-226.[Medline] [Order article via Infotrieve]
  • Baron, R.; Neff, L.; Tran Van, P.; Nefussi, J.; and Vignery, A. (1986): Kinetic and Cytochemical Identification of Osteoclast Precursors and Their Differentiation into Multinucleated Osteoclasts, Am J Pathol 2:363-378.
  • Burstone, M.S. (1958a): Histochemical Comparison of Naphthol AS-Phosphates for the Demonstration of Phosphatase, J Natl Cancer Inst 20:601-615.[Medline] [Order article via Infotrieve]
  • Burstone, M.S. (1958b): Histochemical Demonstration of Acid Phosphatase using Naphthol AS-Phosphates, J Natl Cancer Inst 21:523-539.[Medline] [Order article via Infotrieve]
  • Burstone, M.S. (1960): Histochemical Observation on Enzymatic Processes in Bones and Teeth, Ann NYAcad Sci 85:431-444.[Medline] [Order article via Infotrieve]
  • Cahill, D.R. (1969): Eruption Pathway Formation in the Presence of Experimental Tooth Impaction in Puppies, Anat Rec 164:67-78.[CrossRef][Medline] [Order article via Infotrieve]
  • Cahill, D.R. and Marks, S.C., Jr. (1980): Tooth Eruption: Evidence for the Central Role of the Dental Follicle, J Oral Pathol 9:189-200.[CrossRef][Medline] [Order article via Infotrieve]
  • Carl, W. and Wood, R. (1980): Effects of Radiation on the Developing Dentition and Supporting Bone, J Am Dent Assoc 101:646-648.[Medline] [Order article via Infotrieve]
  • Cole, A.A. and Walters, L.M. (1987): Tartrate-resistant Acid Phosphatase in Bone and Cartilage Following Decalcification and Cold-Embedding in Plastic, J Histochem Cytochem 35:203-206.[Abstract]
  • Engel, M.B. and Furuta, W. (1942): Histochemical Studies of Phosphatase Distribution in Developing Teeth of Albino Rat, Proc Soc Exp Biol Med 50:5-9.[CrossRef]
  • Fleisch, H. (1964): Role of Nucleation and Inhibition of Calcification, Clin Orthop Rel Res 32:170-180.[Medline] [Order article via Infotrieve]
  • Glasstone, S. (1958): The Distribution of Alkaline Phosphatase in Normal and Transplanted Rodent Teeth, Br Dent J 105:58-62.
  • Gowgiel, J.M. (1961): Eruption of Irradiation-produced Rootless Teeth in Monkeys, J Dent Res 40:538-547.[Free Full Text]
  • Gregg, J.M. and Avery, J.K. (1964): Studies of Alveolar Bone Growth and Tooth Eruption Using Tetracycline-induced Fluorescence, J Oral Therap Pharmacol 1:268-281.[Medline] [Order article via Infotrieve]
  • Hammarstrom, L.E.; Hanker, J.S.; and Toverud, S.U. (1971): Cellular Differences in Acid Phosphatase Isoenzymes in Bone and Teeth, Clin Orthop 78:151-167.[CrossRef][Medline] [Order article via Infotrieve]
  • Helminen, H.J.; Ericsson, J.L.E.; Rytoluoto, R.; and Vanha-Perttula, T. (1975): Acid Phosphatases of the Rat Ventral Prostate. In: Normal and Abnormal Growth of the Prostate, M. Goland, Ed., Springfield, IL: Charles C. Thomas, pp. 275-316.
  • Kameyama, Y. (1973): The Pattern of Alveolar Bone Activity During Development and Eruption of the Molar in the Rat, J Periodont Res 8: 179-191.[Medline] [Order article via Infotrieve]
  • Kenny, E.B. and Ramfjord, S.P. (1969): Patterns of Root and Alveolar-Bone Growth Associated with Development and Eruption of Teeth in Rhesus Monkey, J Dent Res 48:251-256.[Abstract/Free Full Text]
  • Kurahashi, Y. and Yoshiki, S. (1972): Electron Microscopic Localization of Alkaline Phosphatase in the Enamel Organ of the Young Rat, Arch Oral Biol 17:155-163.[CrossRef][Medline] [Order article via Infotrieve]
  • Leis, J.F. and Kaplan, N.O. (1982): An Acid Phosphatase in the Plasma Membranes of Human Astrocytoma Showing Marked Specificity Toward Phosphotyrosine Protein, Proc Natl Acad Sci USA 79:6507-6511.[Abstract/Free Full Text]
  • Leonard, E.P. and Provenza, D.V. (1973): Alkaline Phosphatase Activity in Sequential Mouse Molar Tooth Development-An Electron Microscopic Study, Histochemie 34:343-354.[Medline] [Order article via Infotrieve]
  • Lin, C. and Fishman, W.H. (1972): Microsomal and Lysosomal Acid Phosphatase Isoenzymes of Mouse Kidney. Characterization and Separation, J Histochem Cytochem 20:487-498.[Medline] [Order article via Infotrieve]
  • Lunt, D.A. and Noble, H.W. (1972): Localization of Alkaline Phosphatases in Human Cap-stage Enamel Organs by Electron Histochemistry, Arch Oral Biol 17:761-769.[Medline] [Order article via Infotrieve]
  • Lyaruu, D.M.; Woltgens, J.H.M.; and Bervoets, Th.J.M. (1987): Effect of Alkaline-Phosphatase Inhibition by 1-P-Bromotetramisole on the Formation of Trichloroacetic Acid [32P]-insoluble Phosphate from Inorganic [32P]-Phosphate and [32P]-Pyrophosphate in Non-mineralizing and Mineralizing Hamster Molar Tooth-germs in vitro, Arch Oral Biol 32:429-432.[Medline] [Order article via Infotrieve]
  • Marks, S.C., Jr. (1976): Tooth Eruption and Bone Resorption: Experimental Investigation of the ia (Osteopetrotic) Rat as a Model for Studying their Relationships, J Oral Pathol 5:149-163.[Medline] [Order article via Infotrieve]
  • Marks, S.C., Jr. and Cahill, D.R. (1984): Experimental Study in the Dog of the Non-active Role of the Tooth in the Eruptive Process, Arch Oral Biol 29:311-322.[CrossRef][Medline] [Order article via Infotrieve]
  • Marks, S.C., Jr.; Cahill, D.R.; and Wise, G.E. (1983): The Cytology of the Dental Follicle and Adjacent Alveolar Bone During Tooth Eruption in the Dog, Am J Anat 168:277-289.[CrossRef][Medline] [Order article via Infotrieve]
  • Marks, S.C., Jr. and Grolman, M.L. (1987): Tartrate-resistant Acid Phosphatase in Mononuclear and Multinuclear Cells During the Bone Resorption of Tooth Eruption, J Histochem Cytochem 35:1227-1230.[Medline] [Order article via Infotrieve]
  • Minkin, C. (1982): Bone Acid Phosphatase: Tartrate-resistant Acid Phosphatase as a Marker of Osteoclast Function, Calcif Tissue Int 34:285-290.[CrossRef][Medline] [Order article via Infotrieve]
  • Moe, D.; Kirkeby, S.; and Salling, E. (1986): Biochemical Characterization of Alkaline Phosphatase From Partly Mineralized Bovine Enamel, J Biol Buccale 14:249-253.[Medline] [Order article via Infotrieve]
  • Mostafa, Y.A.; Meyer, R.A., Jr.; and Latorraco, R. (1982): A Simple and Rapid Method for Osteoclast Identification Using a Histochemical Method for Acid Phosphatase, Histochem J 14:409-413.[CrossRef][Medline] [Order article via Infotrieve]
  • Nuki, K. and Bonting, S.L. (1961): Quantitative Histochemistry of the Developing Hamster Tooth: Alkaline Phosphatase and Lactic Dehydrogenase, J Histochem Cytochem 9:117-125.[Abstract]
  • Symons, N.B.B. (1955): Alkaline Phosphatase Activity in Developing Teeth of the Rat, J Anat 89:238-241.[Medline] [Order article via Infotrieve]
  • Takano, Y.; Ozawa, H.; and Crenshaw, M.A. (1986): Ca-ATPase and ALPase Activities at the Initial Calcification Sites of Dentin and Enamel in the Rat Incisor, Cell Tissue Res 243:91-99.[Medline] [Order article via Infotrieve]
  • Ten Cate, A.R. (1975): Formation of Supporting Bone in Association with Periodontal Ligament Organization in the Mouse, Arch Oral Biol 20:137-138.[CrossRef][Medline] [Order article via Infotrieve]
  • Van de Wijngaert, F.P. and Burger, E.H. (1986): Demonstration of Tartrate-resistant Acid Phosphatase in Un-decalcified, Glycolmethacrylateembedded Mouse Bone: A Possible Marker for (Pre)Osteoclast Identification, J Histochem Cytochem 34:1317-1323.[Abstract]
  • Wergedal, J.E. (1970): Characterization of Bone Acid Phosphatase Activity, Proc Soc Exp Biol Med 134:244-247.[CrossRef][Medline] [Order article via Infotrieve]
  • Wise, G.E.; Marks, S.C., Jr.; and Cahill, D.R. (1985): Ultrastructural Features of the Dental Follicle Associated with Formation of the Tooth Eruption Pathway in the Dog, J Oral Pathol 14:15-26.[Medline] [Order article via Infotrieve]
  • Wise, G.E.; Marks, S.C., Jr.; Cahill, D.R.; and Gorski, J.P. (1989): Ultrastructure Features of the Dental Follicle and Enamel Organ Prior to and During Tooth Eruption ( accepted for Proceedings of International Conference on Biological Mechanisms of Tooth Eruption and Root Resorption, EBSCO Media).

Journal of Dental Research, Vol. 68, No. 2, 150-156 (1989)
DOI: 10.1177/00220345890680021001
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
G.E. Wise, S. Frazier-Bowers, and R.N. D'Souza
CELLULAR, MOLECULAR, AND GENETIC DETERMINANTS OF TOOTH ERUPTION
Critical Reviews in Oral Biology & Medicine, July 1, 2002; 13(4): 323 - 335.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
G.E. Wise, B.G. Que, and H. Huang
Synthesis and Secretion of MCP-1 by Dental Follicle Cells-Implications for Tooth Eruption
Journal of Dental Research, November 1, 1999; 78(11): 1677 - 1681.
[Abstract] [PDF]

Home page
 JDRHome page
G.E. Wise
The Biology of Tooth Eruption
Journal of Dental Research, August 1, 1998; 77(8): 1576 - 1579.
[PDF]

Home page
 JDRHome page
R.L. Grier IV and G.E. Wise
Inhibition of Tooth Eruption in the Rat by a Bisphosphonate
Journal of Dental Research, January 1, 1998; 77(1): 8 - 15.
[Abstract] [PDF]

Home page
 JDRHome page
G.E. Wise, L. Zhao, and R.L. Grier IV
Localization and Expression of CSF-1 Receptor in Rat Dental Follicle Cells
Journal of Dental Research, June 1, 1997; 76(6): 1244 - 1249.
[Abstract] [PDF]

Home page
 JDRHome page
G.E. Wise, F. Lin, and L. Zhao
Transcription and Translation of CSF-1 in the Dental Follicle
Journal of Dental Research, September 1, 1995; 74(9): 1551 - 1557.
[Abstract] [PDF]

Home page
 CROBMHome page
J. P. Gorski and S. C. Marks Jr
Current Concepts of the Biology of Tooth Eruption
Critical Reviews in Oral Biology & Medicine, January 1, 1992; 3(3): 185 - 206.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
G. D. Roodman
Osteoclast Differentiation
Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(3): 389 - 409.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Wise, G.E.
Right arrow Articles by Fan, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wise, G.E.
Right arrow Articles by Fan, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?