Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Almqvist, H.
Right arrow Articles by Henrikson, C.O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Almqvist, H.
Right arrow Articles by Henrikson, C.O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

In vitro Root Caries Progression Measured by 125I Absorptiometry: Comparison with Chemical Analysis

H. Almqvist

Department of Cariology, Karolinska Institutet, School of Dentistry, Box 4064, 141 04 Huddinge, Sweden

J.S. Wefel

Specialized Caries Research Center, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242

F. Lagerlof

Department of Cariology, Karolinska Institutet, School of Dentistry, Box 4064, 141 04 Huddinge, Sweden

J. Ekstrand

Specialized Caries Research Center, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242

C.O. Henrikson

Department of Oral Radiology, Karolinska Institutet, School of Dentistry, Box 4064, 141 04 Huddinge, Sweden

Radiation from a 125I source and a non-image-forming detector was used for non-destructive measurements of root caries progression. Blocks were cut parallel to the cementum surface of unexposed human roots. These blocks were then individually demineralized in under-saturated calcium phosphate solutions over an 84-hour period. In order for the in vitro root surface demineralization to be followed, the changes in transmission ({Delta}T) through the blocks were measured, by 125I absorptiometry, eight times during the course of the experiment. Chemical analyses of the calcium output ({Delta}Ca) from the blocks into the demineralizing solutions were also performed, and the rate of demineralization (Vdem ) was calculated from these values. The precision of 125I absorptiometry was calculated from 176 duplicate transmission measurements, and the coefficient of variation was found to be 0.20%. The correlation coefficient between {Delta}T and total {Delta}Ca for each of 22 cementumldentin blocks ranged between r = 0.934 and r = 0.998. The progression of root hard-tissue lesions observed by these two methods and by the calculated Vdem was found to be proportional to the square and cubic roots of time. The study shows that 125I absorptiometry can be used for continuous non-destructive measurements of root hard-tissue demineralization in vitro.

REFERENCES

  • Angmar, B.; Carlstrom, D.; and Glas, J.E. (1963): Studies on the Ultrastructure of Dental Enamel. IV. The Mineralization of Normal Human Enamel, J Ultrastruct Res 8:12-23.[Medline] [Order article via Infotrieve]
  • Arends, J.; Christoffersen, J.; Ruben, J.; and Christoffersen, M.R. (1987): Lesion Progress in Dentine and the Role of Fluoride. In: Dentine and Dentine Reactions in the Oral Cavity, A. Thylstrup, S.A. Leach, and V. Qvist, Eds., Oxford: IRL Press Ltd., pp. 117-125.
  • Carlström, D. (1964): Polarization Microscopy of Dental Enamel with Reference to Incipient Carious Lesions. In: Advances in Oral Biology, Vol. 1, P.H. Staple, Ed., New York: Academic Press, pp. 255-296.
  • Christoffersen, J. and Arends, J. (1982): Progress of Artificial Carious Lesions in Enamel, Caries Res 16:433-439.[Medline] [Order article via Infotrieve]
  • De Josselin De Jong, E.; Van Der Linden, A.H.I.M.; and Ten Bosch, J.J. (1987): Longitudinal Microradiography: A Non-Destructive Automated Quantitative Method to Follow Mineral Changes in Mineralised Tissue Slices, Phys Med Biol 32:1209-1220.[CrossRef][Medline] [Order article via Infotrieve]
  • Featherstone, J.D.B.; Duncan, J.F.; and Cutress, T.W. (1979): A Mechanism for Dental Caries Based on Chemical Processes and Diffusion Phenomena during in vitro Caries Simulation on Human Tooth Enamel, Arch Oral Biol 24:101-112.[CrossRef][Medline] [Order article via Infotrieve]
  • Featherstone, J.D.B.; McIntyre, J.M.; and Fu, J. (1987): Physico-chemical Aspects of Root Caries Progression. In: Dentine and Dentine Reactions in the Oral Cavity, A. Thylstrup, S.A. Leach, and V. Qvist, Eds., Oxford: IRL Press Ltd., pp. 127-137.
  • Featherstone, M.J. and Silverstone, L.M. (1982): Creation of Caries-like Lesions in Sections of Teeth Using Acid-gels, IADR Progr & Abst 61: No. 278.
  • Henrikson, C.O. and Linden, L.-A. (1974): Quantitative Longitudinal Study of Enamel Decalcification in vitro, Swed Dent J 67:69-80.
  • Hoppenbrouwers, P.M.M.; Driessens, F.C.M.; and Borg-Greven, J.M.P.M. (1986): The Vulnerability of Unexposed Human Dental Roots to Demineralization, J Dent Res 65:955-958.[Abstract/Free Full Text]
  • Julin, P. (1975): Quantitative Longitudinal Photon Absorptiometry in vitro on Changes in Enamel Mineral Mass. Thesis. Odontological Faculty, Karolinska Institutet, Stockholm, Sweden.
  • Koulourides, T.; Phantumvanit, J.; Munksgaard, E.C.; and Housch, T. (1974): An Intra-oral Model used for Studies of Fluoride Incorporation in Enamel, J Oral Pathol 3:185-195.[CrossRef][Medline] [Order article via Infotrieve]
  • Lagerlof, F. (1983): Effects of Flow Rate and pH on Calcium Phosphate Saturation in Human Parotid Saliva, Caries Res 17:403-411.[Medline] [Order article via Infotrieve]
  • Phankosol, P.; Ettinger, R.L.; Hicks, M.J.; and Wefel, J.S. (1985): Depth of Penetration of in vitro Root Surface Lesions, J Dent Res 64:897-899.[Abstract/Free Full Text]
  • Poole, D.F.G.; Shellis, R.P.; and Tyler, J.E. (1981): Rates of Formation in vitro of Dental Caries-like Enamel Lesions in Man and Some Non-human Primates, Arch Oral Biol 26:413-417.[CrossRef][Medline] [Order article via Infotrieve]
  • Ten Bosch, J.J.; Van Der Mei, H.C.; and Borsboom, P.C.F. (1984): Optical Monitor of in vitro Caries. A Comparison with Chemical and Microradiographical Determination of Mineral Loss in Early Lesions, Caries Res 18:540-547.[Medline] [Order article via Infotrieve]
  • Ten Cate, J.M. and Duijsters, P.P.E. (1983): Influence of Fluoride in Solution on Tooth Demineralization. I. Chemical Data, Caries Res 17:193-199.
  • Theuns, H.M.; Van Dijk, J.W.E.; Driessens, F.C.M.; and Groeneveld, A. (1985): Effect of Time, Degree of Saturation, pH and Acid Concentration of Buffer Solutions on the Rate of in vitro Demineralization of Human Enamel, Arch Oral Biol 30:37-42.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 67, No. 9, 1217-1220 (1988)
DOI: 10.1177/00220345880670091301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
H. Almqvist and F. Lagerlof
Effect of Intermittent Delivery of Fluoride to Solution on Root Hard-tissue De- and Remineralization Measured by 125I Absorptiometry
Journal of Dental Research, December 1, 1993; 72(12): 1593 - 1598.
[PDF]

Home page
 JDRHome page
J.M. Ten Cate, B. Nyvad, Y.M. Van De Plassche-Simons, and O. Fejerskov
A Quantitative Analysis of Mineral Loss and Shrinkage of in vitro Demineralized Human Root Surfaces
Journal of Dental Research, October 1, 1991; 70(10): 1371 - 1374.
[Abstract] [PDF]

Home page
 JDRHome page
J.J. Ten Bosch and B. Angmar-Mansson
Invited Review: A Review of Quantitative Methods for Studies of Mineral Content of Intra-oral Incipient Caries Lesions
Journal of Dental Research, January 1, 1991; 70(1): 2 - 14.
[Abstract] [PDF]

Home page
 JDRHome page
H. Almqvist, J.S. Wefel, and F. Lagerlof
Root Hard-tissue Demineralization Rate Measured by 125I Absorptiometry: Comparison with Lesion-depth Measurements
Journal of Dental Research, August 1, 1990; 69(8): 1519 - 1521.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Almqvist, H.
Right arrow Articles by Henrikson, C.O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Almqvist, H.
Right arrow Articles by Henrikson, C.O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?