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Production of Interleukin-1 and Tumor Necrosis Factor by Human Peripheral Monocytes Activated by Periodontal Bacteria and Extracted Lipopolysaccharides
R.A. Lindemann
Section of Oral Diagnosis, Oral Medicine, and Oral Pathology, UCLA School of Dentistry and the Dental Research Institute
J.S. Economou
Division of Surgical Oncology, UCLA School of Medicine, Center for the Health Sciences, Los Angeles, California 90024
H. Rothermel
Division of Surgical Oncology, UCLA School of Medicine, Center for the Health Sciences, Los Angeles, California 90024
Whole Gram-negative bacteria associated with juvenile and adult periodontitis, and their respective extracted lipopolysaccharides (LPS), were tested for the ability to activate quiescent human peripheral blood monocytes. All pathogenic Gram-negative bacteria and all LPS tested were able to induce the production of significant amounts of IL-1 and TNF, monokines known to induce osteoclastic bone resorption. Haemophilus segnis, which has not been associated with any form of periodontal disease, did not activate monocytes. Purified LPS from Actinobacillus actinomycetemcomitans Y4 was able to elicit IL-1 and TNF release at a threshold concentration of 1-10 ng/mL. To examine the mechanism whereby whole bacteria activated monocytes, we added polymixin B in culture with glutaraldehyde-fixed bacteria to bind LPS. This resulted in the abrogation of IL-1 and TNF production. To compare the effects of Gram-positive oral bacteria on monocytes, we also tested Staphylococcus epidermidis and the Gram-positive amphipathic equivalent of LPS, lipoteichoic acid (LTA) extracted from Staphylococcus aureus bacteria. Whereas whole Gram-positive bacteria had no stimulatory effect on monocytes, LTA induced IL-1 and TNF production at a concentration range equivalent to that of the LPS. These results indicate that monocytes are activated by free LPS or LPS bound to Gram-negative pathogenic periodontal bacteria to produce monokines which may contribute to the destruction of periodontal bone.
REFERENCES
- Alexander, P. and Evans, R. (1971): Endotoxin and Double Stranded RNA Render Macrophages Cytotoxic, Nature New Biol 232:76-78.[Medline]
[Order article via Infotrieve]
- Bertolini, D.R.; Nedwin, G.E.; Bringman, T.S.; Smith, D.D.; and Mundy, G.R. (1986): Stimulation of Bone Resorption and Inhibition of Bone Formation in vitro by Human Tumor Necrosis Factors, Nature 319:516-518.[CrossRef][Medline]
[Order article via Infotrieve]
- Cameron, D.J. and Churchill, W.H. (1980): Cytotoxicity of Human Macrophages for Tumor Cells: Enhancement by Bacterial Lipopolysaccharides (LPS), J Immunol 124:708-712.[Medline]
[Order article via Infotrieve]
- Dewhirst, F.E.; Ago, J.M.; and Stashenko, P. (1987): Interleukin 1 and Prostaglandin E2 are Synergistic in Stimulating Bone Resorption, J Dent Res 66:122, Abst. No. 123.
- Dewhirst, F.E.; Stashenko, P.P.; Mole, J.E.; and Tsu-Rumachi, T. (1985): Purification and Partial Sequence of Human Osteoclast-activating Factor: Identity with Interleukin 1-beta, J Immunol 135:2562-2568.[Abstract]
- Dinarello, C.A.; Cannon, J.G.; Wolff, S.M.; Bernheim, H.A.; Beutler, B.; Cerami, A.; Figari, I.S.; Palla-Dino, M.A., Jr.; and O'Connor, J.V. (1986): Tumor Necrosis Factor (Cachectin) is an Endogenous Pyrogen and Induces Production of Interleukin 1, J Exp Med 163:1433-1450.[Abstract/Free Full Text]
- Economou, J.S.; McBride, W.; Golub, S.H.; and Morton, D.L. (1987): Interleukin-1-alpha (IL-1-alpha) and IL-1-beta Stimulate Peripheral Adherent Mononuclear Cells to Produce a Tumor Necrosis Factor (TNF)-Like Monocytotoxin, Proc Am Assoc Cancer Res 28:344, Abst. No. 1361.
- Economou, J. and Shin, H. (1978): Lymphocyte-activating Factor. Generation and Physiochemical Characterization, J Immunol 121:1446-1452.[Abstract/Free Full Text]
- Elias, J.A.; Gustillo, K.; Baeder, W.; and Freundlich, B. (1987): Synergistic Stimulation of Fibroblast Prostaglandin Production by Recombinant Interleukin-1 and Tumor Necrosis Factor, J Immunol 138:3812-3816.[Abstract]
- Gowen, M.M.; Meikle, C.; and Reynolds, J.J. (1983a): Stimulation of Bone Resorption in vitro by a Non-prostanoid Factor Released by Human Monocytes in Culture, Biochim Biophys Acta 762:471-474.[Medline]
[Order article via Infotrieve]
- Gowen, M.; Wood, D.D.; Ihrie, E.J.; McGuire, M.K.B.; and Russell, R.G.G. (1983b): An Interleukin-1 Like Factor Stimulates Bone Resorption in vitro, Nature 306:378-380.[CrossRef][Medline]
[Order article via Infotrieve]
- Hanazawa, S.; Nakada, K.; Ohmori, Y.; Miyoshi, T.; Amano, S.; and Kitano, S. (1985): Functional Role of Interleukin 1 in Periodontal Disease: Induction of Interleukin 1 Production by Bacteroides gingivalis Lipopolysaccharide in Peritoneal Macrophages from C3H/HeN and C3H/HeJ Mice, Infect Immun 50:262-270.[Abstract/Free Full Text]
- Hausmann, E.; Chandran Nair, B.; and Dziak, R. (1982): Bacterial Components Which Result in Bone Loss. In: Host-Parasite Interactions in Periodontal Diseases, R.J. Genco and S.E. Mergenhagen, Eds., Washington, D.C.: American Society for Microbiology, pp. 151-159.
- Humes, J.L.; Bonney, R.J.; Pelus, L.; Dahlgren, M.E.; Sadowski, S.J.; Kuehl, F.A., Jr.; and Davies, P. (1977): Macrophages Synthesize and Release Prostaglandins in Response to Inflammatory Stimuli, Nature 269:149-151.[CrossRef][Medline]
[Order article via Infotrieve]
- Jandinsky, J.; Leung, C.; Macedo, B.; Rynar, J.E.; Deasy, M.J.; and Stashenko, P. (1988): Localization of Interleukin-1 beta in Human Gingival Tissue, J Dent Res 67:401, Abst. No. 2307.
- Johansson, O.E.; Christersson, L.A.; Johansson, I.M.; Zambon, J.J.; and Genco, R.J. (1987): Specific Microorganisms and Diagnosis of Adult Periodontitis, J Dent Res 66:120, Abst. No. 105.
- Millar, S.J.; Goldstein, E.G.; Levine, M.J.; and Hausmann, E. (1986): Modulation of Bone Metabolism by Two Chemically Distinct Lipopolysaccharide Fractions from Bacteroides gingivalis, Infect Immun 51:302-306.[Abstract/Free Full Text]
- Morrison, D.C. and Jacobs, D.M. (1976): Binding of Polymixin B to the Lipid A Portion of Bacterial Polysaccharides, Immunochem 13:813-818.[CrossRef][Medline]
[Order article via Infotrieve]
- Newton, R.C. (1986): Human Monocyte Production of Interleukin-1: Parameters of the Induction of Interleukin-1 Secretion by Lipopolysaccharides, J Leuk Biol 39:299-311.[Abstract]
- Offenbacher, S.; Binder, T.A.; Dewhirst, F.E.; and Goodson, J.M. (1981): Bone Resorptive Activity of Human Periodontal Tissues, J Dent Res 60:500, Abst. No. 762.
- Offenbacher, S.; Pinnix, K.; and Odle, B. (1988): The Measurement of Tumor Necrosis Factor in Inflamed Periodontal Tissues, J Dent Res 67:156, Abst. No. 346.
- Old, L.J. (1985): Tumor Necrosis Factor, Science 230:630-632.[Free Full Text]
- Oppenheim, J.J.; Kovacs, E.J.; Matsushima, K.; and Durum, S.K. (1986): There is More Than One Interleukin-1, Immunol Today 7:45-56.[CrossRef]
- Postlethwaite, A.E.; Lachman, L.B.; Mainaldi, C.L.; and Kang, A.H. (1983): Interleukin-1 Stimulation of Collagenase Production by Cultured Fibroblasts, J Exp Med 157:801-806.[Abstract/Free Full Text]
- Rifkind, D. and Hill, R.B., Jr. (1967): Neutralization of the Shwartzman Reactions by Polymixin B, J Immunol 99:564-569.[Abstract/Free Full Text]
- Russell, S.W.; Pace, J.L.; Varesio, L.; Akporiaye, E.; Blasi, E.; Celado, A.; Schreiber, R.D.; Schultz, R.M.; Stevenson, A.P.; Stewart, C.C.; and Stewart, S.J. (1986): Comparison of Five Short-term Assays that Measure Nonspecific Cytotoxicity Mediated to Tumor Cells by Activated Macrophages, J Leuk Biol 40:801-813.[Medline]
[Order article via Infotrieve]
- Saglie, F.R.; Carranza, F.A., Jr.; Newman, M.G.; Cheng, L.; and Lewin, K.J. (1982): Identification of Tissue-invading Bacteria in Human Periodontal Disease, J Periodont Res 17:452-455.[CrossRef][Medline]
[Order article via Infotrieve]
- Slots, J. and Genco, R.J. (1984): Black-pigmented Bacteroides Species, Capnocytophaga Species, and Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Virulence Factors in Colonization, Survival, and Tissue Destruction, J Dent Res 63:412-421.[Free Full Text]
- Tanner, A.C.R.; Badger, S.; Lai, C.-H.; Listgarten, M.A.; Visconti, R.A.; and Socransky, S.S. (1981): Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and Description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from Humans with Periodontal Disease, Int J Syst Bacteriol 31:432-445.
- Thomson, B.M.; Mundy, G.R.; and Chambers, T.J. (1987): Tumor Necrosis Factors Alpha and Beta Induce Osteoblastic Cells to Stimulate Osteoclastic Bone Resorption, J Immunol 138:775-779.[Abstract]
- Westphal, O. and Jann, K. (1965): Bacterial Lipopolysaccharides. In: Methods in Carbohydrate Chemistry, R. Whistler, Ed., New York: Academic Press, pp. 83-92.
- Zambon, J.J.; Christersson, L.A.; and Slots, J. (1983): Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Prevalence in Patient Groups and Distribution of Biotypes and Serotypes within Families, J Periodontol 54:707-711.[Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 67, No. 8,
1131-1135 (1988)
DOI: 10.1177/00220345880670081401
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