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Journal of Dental Research
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Attachment of Bacteroides gingivalis to Collagenous Substrata

Y. Naito

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

R.J. Gibbons

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

The ability of Bacteroides gingivalis 381 to attach to hydroxyapatite (HA) beads, treated with either human type I or type IV collagen, or to particles of bovine bone collagen was studied. All preparations were blocked with human albumin prior to being incubated with 3H-thymidine-labeled B. gingivalis 381 cells. The presence of collagen on HA surfaces (C-HA) significantly promoted attachment of the organism. HA treated with Type IV collagen bound B. gingivalis cells more effectively than did HA treated with type I collagen. Attachment of two additional strains of B. gingivalis to HA was also promoted by collagen. Binding to type I or type IV C-HA occurred rapidly, and equilibrium was attained within 45 min. B. gingivalis 381 cells also bound to particles of bovine bone collagen, and this appeared to be biphasic. Heating the bacteria abolished their ability to bind to C-HA. Attachment of B. gingivalis 381 cells to HA treated with type I collagen was strongly inhibited by the presence of soluble type I or type IV collagen, or gelatin, but not by the presence of human albumin, salivary proline-rich protein 1, or saliva. Human serum, fibronectin, fibrinogen, certain protease inhibitors, and some peptides were also inhibitory. 3H-fibronectin bound to bovine bone collagen particles and blocked the attachment of 14C-B. gingivalis cells. Mild trypsin treatment of the fibronectin-collagen complex restored its ability to promote 14C-B. gingivalis attachment concomitant with the loss of 3H-fibronectin. We suggest that elevated levels of proteases in the gingival sulcus, such as are associated with poor oral hygiene and gingivitis, might remove fibronectin and expose collagen molecules in the basement membrane, thereby promoting the attachment of B. gingivalis cells and facilitating their invasion into gingival tissues.

REFERENCES

  • Brissette, R.; Gibbons, R.J.; Hay, D.I.; and Schluckebier, S. (1986): Attachment of Bacteroides gingivalis 381 to Salivary Proline-rich Proteins on Hydroxyapatite Surfaces, J Dent Res 66:286.
  • Childs, W.C. and Gibbons, R.J. (1986): Use of Percoll Gradients for Assays of Bacterial Attachment to Epithelial Cells, J Dent Res 65:736.
  • Courant, P.R. and Bader, H. (1966): Bacteroides melaninogenicus and Its Products in the Gingiva of Man, J Periodontol 4:131-136.
  • Crawford, A.C.R.; Socransky, S.S.; Smith, E.; and Phillips, R. (1977): Pathogenicity Testing of Oral Isolates in Gnotobiotic Rats, J Dent Res 56:275.[Abstract/Free Full Text]
  • Ebersole, J.L.; Taubman, D.J.; Smith, D.J.; and Frey, D.E. (1986): Human Immune Response to Oral Microorganisms: Patterns of Systemic Antibody Levels to Bacteroides Species, Infect Immun 51:507-513.[Abstract/Free Full Text]
  • Gibbons, R.J. and Etherden, I. (1985): Albumin as a Blocking Agent in Studies of Streptococcal Adsorption to Experimental Pellicles, Infect Immun 50:592-594.[Abstract/Free Full Text]
  • Gibbons, R.J. and Etherden, I. (1986): Fibronectin-degrading Enzymes in Saliva and their Relation to Oral Hygiene, J Periodont Res 21:386-395.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons, R.J. and MacDonald, J.B. (1961): Degradation of Collagenous Substrates by Bacteroides melaninogenicus, J Bacteriol 81:614-621.[Free Full Text]
  • Grant, M.E.; Heathcote, J.G.; and Orkin, R.W. (1981): Current Concepts of Basement Membrane Structure and Function, Biosci Rep 1:819-842.[CrossRef][Medline] [Order article via Infotrieve]
  • Hausmann, E. and Haufman, E. (1969): Collagenase Activity in a Particulate Fraction from Bacteroides melaninogenicus, Biochim Biophys Acta 194:612-615.[Medline] [Order article via Infotrieve]
  • Heijl, L.; Wennstrom, J.; Lindhe, J.; and Socransky, S.S. (1980): Periodontal Disease in Gnotobiotic Rats, J Periodont Res 15:405-419.[CrossRef][Medline] [Order article via Infotrieve]
  • Holderbaum, D.; Hall, G.S.; and Ehrhart, L.A. (1986): Collagen Binding to Staphylococcus aureus, Infect Immun 54:359-364.[Abstract/Free Full Text]
  • Inoshita, E.; Amano, A.; Hanioka, T.; Tamagawa, H.; Shizu-Kuishi, S.; and Tsunemitsu, A. (1986): Isolation and Some Properties of Exohemagglutinin from Culture Medium of Bacteroides gingivalis 381, Infect Immun 52:421-427.[Abstract/Free Full Text]
  • Jentoft, N. and Dearborn, D.G. (1979): Labeling of Proteins by Reductive Methylation Using Sodium Cyanoborohydride, J Biol Chem 254:4359-4365.[Free Full Text]
  • Karp, W.; Sodek, J.; Aubin, J.E.; and Melcher, A.H. (1986): A Comparison of Fibronectin and Laminin Binding to Undemineralized and Demineralized Tooth Root Surfaces, J Periodont Res 21:30-38.[Medline] [Order article via Infotrieve]
  • Kowaski, Y.; Jaccard, F.; and Cimasoni, G. (1979): Increase of Free Collagenase and Neutral Protease Activities in the Gingival Crevice During Experimental Gingivitis in Man, Arch Oral Biol 249:645-650.
  • Lantz, M.S.; Rowland, R.W.; Switalski, L.M.; and Hook, M. (1986): Interaction of Bacteroides gingivalis with Fibrinogen, Infect Immun 54:654-658.[Abstract/Free Full Text]
  • Mosher, D.F. (1980): Fibronectin, Prog Hemostasis Thrombosis 5:111-151.
  • Mouton, C.; Hammond, P.; and Genco, R.J. (1981): Serum Antibodies to Oral Bacteroides asaccharolyticus (Bacteroides gingivalis): Relationship to Age and Periodontal Disease, Infect Immun 311:182-192.
  • Nagahata, T.; Kiyoshige, T.; Abe, T.; Sasaki. S.; and Takazoe, I. (1981): Oral Establishment of Bacteroides asaccharolyticus and Eikenella corrodens in Conventional Hamsters, Infect Immun 36:304-309.
  • Naito, Y.; Okuda, K.; Takazoe, I.; Watanabe, H.; and Ishikawa, I. (1985): The Relationship Between Serum IgG Levels to Subgingival Gram-negative Bacteria and Degree of Periodontal Destruction, J Dent Res 64:1306-1310.[Abstract/Free Full Text]
  • Okuda, K.; Yamamoto, A.; Naito, Y.; Takazoe, I.; Slots, J.; and Genco, R.J. (1986): Purification and Properties of Hemagglutinin from Culture Supernatant of Bacteroides gingivalis, Infect Immun 54:659-665.[Abstract/Free Full Text]
  • Pearlstein, E.; Gold, L.I.; and Garcia-Pardo, A. (1980): Fibronectin: A Review of Its Structure and Biological Activity, Molecular and Cellular Biochem 29:103-128.
  • Pekovic, D.D. and Fillery, E.D. (1986): Identification of Bacteria in Immunopathological Mechanisms of Human Periodontal Diseases, J Periodont Res 19:329-351.
  • Sandholm, L. (1986): Proteases and Their Inhibitors in Chronic Inflammatory Periodontal Disease, J Clin Periodontol 13:19-26.[CrossRef][Medline] [Order article via Infotrieve]
  • Slots, J. (1977): The Predominant Cultivable Microflora of Advanced Periodontitis, Scand J Dent Res 85:114-121.[Medline] [Order article via Infotrieve]
  • Slots, J. (1979): Subgingival Microflora and Periodontal Disease, J Clin Periodontol 6:351-382.[CrossRef][Medline] [Order article via Infotrieve]
  • Slots, J. and Genco, R.J. (1985): Black-pigmented Bacteroides Species, Capnocytophoga Species, and Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Virulence Factors in Colonization, Survival, and Tissue Destruction, J Dent Res 63:412-421.
  • Slots, J. and Gibbons, R.J. (1978): Attachment of Bacteroides melaninogenicus Subsp. asaccharolyticus to Oral Surfaces and Its Possible Role in Colonization of the Mouth and of Periodontal Pockets, Infect Immun 19:256-264.
  • Socransky, S.S. and Gibbons, R.J. (1965): Required Role of Bacteroides melaninogenicus in Mixed Anaerobic Infections, J Infect Dis 115:247-253.[Medline] [Order article via Infotrieve]
  • Spiegel, C.A.; Hayduk, S.E.; Minah, G.E.; and Krywolap, G.E. (1979): Black-pigmented Bacteroides from Clinically Characterized Periodontal Sites, J.Periodont Res 14:376-382.
  • Suido, H.; Neiders, M.E.; Barau, P.K.; Nakamura, M.; Mash-Imo, P.A.; and Genco, R.J. (1987): Characterization of N-CBz-glycyl-glycyl-arginyl Peptidase and Glycyl-prolyl Peptidase of Bacteroides gingivalis, J Periodont Res 22:412-418.[CrossRef][Medline] [Order article via Infotrieve]
  • Tanner, A.C.R.; Haffajee, C.; Bratthall, G.T.; Visconti, R.A.; and Socransky, S.S. (1979): A Study of the Bacteria Associated with Advancing Periodontitis in Man, J Clin Perio 6:278-307.
  • Toda, K.; Otsuka, M.; Ishikawa, Y.; Sato, M.; Yamamoto, Y.; and Nakamura, R. (1984): Thiol-dependent Collagenolytic Activity in Culture Media of Bacteroides gingivalis, J Periodont Res 19:372-381.[CrossRef][Medline] [Order article via Infotrieve]
  • Uitto, V. (1983): Degradation of Basement Membrane Collagen by Proteinase from Human Gingiva, Leukocytes and Bacterial Plaque, J Periodontol 56:740-745.
  • White, D. and Mayrand, D. (1981): Association of Oral Bacteroides with Gingivitis and Adult Periodontitis, J Periodont Res 16:259-265.[CrossRef][Medline] [Order article via Infotrieve]
  • Yamada, K.M. and Olden, K. (1978): Fibronectin-adhesive Glycoproteins of Cell Surface and Blood, Nature 275:179-184.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 67, No. 8, 1075-1080 (1988)
DOI: 10.1177/00220345880670080301
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