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Journal of Dental Research
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Electrophoretic Heterogeneity of Lipopolysaccharides of Actinobacillus actinomycetemcomitans

C.I. Hoover

Department of Stomatology, University of California, San Francisco, California 94143

Electrophoretic banding patterns of lipopolysaccharides (LPS), as observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), have proved to be useful in studies relating LPS structure to virulence and as epidemiological markers. In this report, LPS of Actinobacillus actinomycetemcomitans from outer membrane fractions and hot phenol-water extracts were analyzed by SDS-PAGE and LPS-specific silver-staining techniques. Both intra- and inter-strain heterogeneity of A. actinomycetemcomitans LPS was observed. Twelve strains of A. actinomycetemcomitans, representative of the three described serotypes, were assigned to LPS subtypes based on the relative mobility of their most rapidly migrating LPS band. All three serotype a strains (29523, aB75, and GA3), two (29524 and SAC11A) of five serotype b strains, and two (aB67 and SAC5A) of four serotype c strains were assigned to LPS subtype I. The three remaining serotype b strains (29522, Y4, and JP2) were assigned to LPS subtype II, and the remaining two serotype c strains (SAC6A and SAC12A) were assigned to LPS subtype III. LPS subtyping may serve as an adjunct or alternative to serotyping in epidemiological studies.

REFERENCES

  • Hitchcock, P.J. (1984): Analyses of Gonococcal Lipopolysaccharide in Whole-cell Lysates of Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis: Stable Association of Lipopolysaccharide with the Major Outer Membrane Protein (Protein I) of Neisseria gonorrhoeae, Infect Immun 46:202-212.[Abstract/Free Full Text]
  • Hitchcock, P.J. and Brown, T.M. (1983): Morphological Heterogeneity among Salmonella Lipopolysaccharide Chemotypes in Silver-stained Polyacrylamide Gels, J Bacteriol 154:269-277.[Abstract/Free Full Text]
  • Inzana, T.J. (1983): Electrophoretic Heterogeneity and Interstrain Variation of the Lipopolysaccharide of Haemophilus influenzae, J Infect Dis 148:492-499.[Medline] [Order article via Infotrieve]
  • Inzana, T.J. and Pichichero, M.E. (1984): Lipopolysaccharide Subtypes of Haemophilus influenzae Type b from an Outbreak of Invasive Disease, J Clin Microbiol 20:145-150.[Abstract/Free Full Text]
  • Kusecek, B.; Wolch, H.; Mercer, A.; Vaisanen, V.; Pluschka, G.; Korhoren, T.; and Achtman, M. (1984): Lipopolysaccharide Capsule, and Fimbriae as Virulence Factors among 01,07,016,018, or 075 and K1,K5, or K100 Escherichia coli, Infect Immun 43:368-379.[Abstract/Free Full Text]
  • Laemmli, U.K. (1970): Cleavage of Structural Proteins during Assembly of the Head of Bacteriophage T4, Nature 227:680-685.[CrossRef][Medline] [Order article via Infotrieve]
  • Leive, L.L. and Jimenez-Lucho, V.E. (1986): Lipopolysaccharide O-Antigen Structure Controls Alternate Pathway Activation of Complement: Effects on Phagocytosis and Virulence of Salmonellae. In: Microbiology-1986, L. Leive, Ed., Washington, DC: American Society for Microbiology, pp. 14-17.
  • Luderitz, O. (1977): Endotoxins and other Cell Wall Components of Gram-negative Bacteria and their Biological Activities. In: Microbiology-1977, D. Schlessinger, Ed., Washington, DC: American Society for Microbiology, pp. 239-246.
  • Moreno, E.; Pitt, M.W.; Jones, L.M.; Schurig, G.C.; and Berman, D.T. (1979): Purification and Characterization of Smooth and Rough Lipopolysaccharides from Brucella abortus, J Bacteriol 138:361-369.[Abstract/Free Full Text]
  • Morse, S.A.; Mintz, C.S.; Sarafian, S.K.; Bartenstein, L.; Bertram, M.; and Apicella, M.A. (1983): Effect of Dilution Rate on Lipopolysaccharides and Serum Resistance of Neisseria gonorrhoeae Grown in Continuous Culture, Infect Immun 41:74-82.[Abstract/Free Full Text]
  • Neter, E. (1969): Endotoxins and the Immune Response, Curr Top Microbiol Immunol 47:82-124.[Medline] [Order article via Infotrieve]
  • Nishihara, T.; Fujiwara, T.; Koga, T.; and Hamada, S. (1986): Chemical Composition and Immunological Properties of Lipopolysaccharides and Lipid-associated Proteoglycan from Actinobacillus actinomycetemcomitans, J Periodont Res 21:521-530.[CrossRef][Medline] [Order article via Infotrieve]
  • Nishihara, T.; Koga, T.; and Hamada, S. (1987): Extracellular Proteinaceous Substances from Haemophilus actinomycetemcomitans Induce Mitogenic Responses in Murine Lymphocytes, Oral Microbiol Immunol 2:48-52.[Medline] [Order article via Infotrieve]
  • Palva, E.T. and Makela, P.H. (1980): Lipopolysaccharide Heterogeneity in Salmonella typhimurium Analyzed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, Eur J Biochem 53:137-143.
  • Poolman, J.T.; Hopman, C.T.P.; and Zanen, H.C. (1985): Colony Variants of Neisseria meningitidis Strain 2996(B:2b6:P1.2): Influence of Class-5 Outer Membrane Proteins and Lipopolysaccharides, J Med Microbiol 19:203-209.[Abstract/Free Full Text]
  • Potts, T.V.; Zambon, J.J.; and Genco, R.J. (1985): Reassignment of Actinobacillus actinomycetemcomitans to the Genus Haemophilus as Haemophilus actinomycetemcomitans comb. nov, Int J System Bacteriol 35:337-341.[Abstract/Free Full Text]
  • Progulske, A. and Holt, S.C. (1984): Isolation and Characterization of the Outer Membrane and Lipopolysaccharide from Eikenella corrodens, Infect Immun 43:166-177.[Abstract/Free Full Text]
  • Schnaitman, C.A. (1971): Solubilization of the Cytoplasmic Membrane of Escherichia coli by Triton X-100, J Bacteriol 104:890-901.
  • Schneider, H.; Hale, T.L.; Zollinger, W.D.; Seid, R.C., Jr.; Hammack, G.A.; and Griffiss, J.M. (1984): Heterogeneity of Molecular Size and Antigenic Expression within Lipooligosaccharides of Individual Strains of Neisseria gonorroheae and Neisseria meningitidis, Infect Immun 45:544-549.[Abstract/Free Full Text]
  • Schneider, H.; Hammack, G.A.; Shuman, B.A.; and Griffiss, J.M. (1986): Stability of Expression of Neisseria gonorrhoeae Lipooligosaccharides, Infect Immun 54:924-927.[Abstract/Free Full Text]
  • Slots, J. (1982): Selective Medium for Isolation of Actinobacillus actinomycetemcomitans, J Clin Microbiol 15:606-609.[Abstract/Free Full Text]
  • Taylor, P.W. (1983): Bactericidal and Bacteriolytic Activity of Serum against Gram-negative Bacteria, Microbiol Rev 47:46-83.[Free Full Text]
  • Tsai, C.-M.; Boykins, R.; and Frasch, C.E. (1983): Heterogeneity and Variation among Neisseria meningitidis Lipopolysaccharides, J Bacteriol 155:498-504.[Abstract/Free Full Text]
  • Tsai, C.-M. and Frasch, C.E. (1982): A Sensitive Silver Stain for Detecting Lipopolysaccharides in Polyacrylamide Gels, Anal Biochem 119:115-119.[CrossRef][Medline] [Order article via Infotrieve]
  • Westphal, O. and Jann, K. (1965): Bacterial Lipopolysaccharides: Extraction with Phenol-water and Further Applications of the Procedure. In: Methods in Carbohydrate Chemistry, Vol. 5, R.L. Whistler, J.N. BeMiller, and M.L. Wolfrom, Eds., New York: Academic Press, Inc., pp. 83-91.
  • Westphal, O.; Jann, K.; and Himmelspach, K. (1983): Chemistry and Immunochemistry of Bacterial Lipopolysaccharides as Cell Wall Antigens and Endotoxins, Prog Allergy 33:9-39.[Medline] [Order article via Infotrieve]
  • Zambon, J.J. (1985): Actinobacillus actinomycetemcomitans in Human Periodontal Disease, J Clin Periodontol 12:1-20.[CrossRef][Medline] [Order article via Infotrieve]
  • Zambon, J.J.; Slots, J.; and Genco, R.J. (1983): Serology of Oral Actinobacillus actinomycetemcomitans and Serotype Distribution in Human Periodontal Disease, Infect Immun 41:19-27.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 67, No. 3, 574-576 (1988)
DOI: 10.1177/00220345880670030901
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This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
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Right arrow Citing Articles via Google Scholar
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What's this?