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Histochemical Demonstration of Carbonic Anhydrase Activity in the Odontogenic Cells of the Rat Incisor

2nd Department of Oral Anatomy, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565

Y. Ogawa

Department of Oral Pathology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565

H. Kuwahara

2nd Department of Pathology, Osaka City University Medical School, 1-4-54 Asahimachi, Abenoku, Osaka 545, Japan

M. Shimazaki

2nd Department of Pathology, Osaka City University Medical School, 1-4-54 Asahimachi, Abenoku, Osaka 545, Japan

T. Yagi

Department of Oral Pathology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565

A. Sakai

2nd Department of Oral Anatomy

Aldehyde-fixed, EDTA-demineralized frozen sections of the rat maxillary incisor were histochemically stained for carbonic anhydrase activity, by use of Hansson's method. Intense staining was observed in the odontoblasts, all types of epithelial cells of enamel organ in the maturation zone, cementoblasts, and the cells of the lingual dental sac. Less intense but consistent staining was observed in all types of epithelial cells of odontogenic origin directly facing the pulp and pulp cells adjacent to the odontoblast cell layer in the apical part of the pulp, and was considered due to the carbonic anhydrase-catalyzed reaction. Staining of these cells was completely inhibited by heat pretreatment (120°C, 30 min), 10^-6 mol/L acetazolamide in the incubation medium, incubation by continuous immersion under the liquid surface, and omission of the substrate, NaHCO₃. The dentin also exhibited heavy staining which was inhibited by the heat pre-treatment. However, this dentinal staining resisted the inhibition by 10^-3 mol/L acetazolamide and was not inhibited by incubation by continuous immersion or incubation without the substrate NaHCO₃. The dentinal staining was thus judged to have been due to non-enzymatic cobalt precipitation.

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Journal of Dental Research, Vol. 67, No. 10, 1271-1274 (1988)
DOI: 10.1177/00220345880670100501


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