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Journal of Dental Research
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Methotrexate-induced Changes in Rat Parotid and Submandibular Gland Function

R.K. McBride

Present address, Pulmonary and Critical Care Unit-Box 692, University of Rochester Medical Center, 601 Elmwood, Rochester, New York 14642

C. Harper

Department of Pharmacology, University of Illinois College of Medicine at Urbana-Champaign, 190 Medical Sciences Building, 506 S. Mathews Ave., Urbana, Illinois 61801

I.A. Siegel

Department of Pharmacology, University of Illinois College of Medicine at Urbana-Champaign, 190 Medical Sciences Building, 506 S. Mathews Ave., Urbana, Illinois 61801

Methotrexate (MTX) was injected intraperitoneally at a dose of 15 mglkg into adult rats daily for three consecutive days. When compared with saline-injected, ad libitum-fed rats or pair-fed saline-injected rats, MTX decreased unstimulated parotid gland weight, RNA content, and amylase content. RNA content was also decreased in submandibular glands. The pqrotid and submandibular gland outputs of sodium and chloride, but not that of potassium, were decreased following MTX injection. Ductal perfusion demonstrated decreased net efflux of sodium and chloride from the excretory duct of the submandibular gland. The effects of MTX on unstimulated parotid gland weight, RNA content, and amylase content are consistent with its ability to inhibit protein synthesis through depletion of folate co-factors. However, the mechanism by which the alterations in ion transport occurred is not clear at this time.

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Journal of Dental Research, Vol. 66, No. 9, 1445-1448 (1987)
DOI: 10.1177/00220345870660090701
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