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Diffusion of Fluoride Through the Rat Enamel Organ in vitro
J.W. Bawden
Department of Pediatric Dentistry and the Dental Research Center, University of North Carolina, Chapel Hill, North Carolina 27514
T.G. Deaton
Department of Pediatric Dentistry and the Dental Research Center, University of North Carolina, Chapel Hill, North Carolina 27514
M.A. Crenshaw
Department of Pediatric Dentistry and the Dental Research Center, University of North Carolina, Chapel Hill, North Carolina 27514
This study investigated the diffusion of fluoride through the enamel organ in vitro. The rat molar explants used were entirely in the secretory stage or predominantly in the maturation stage of enamel formation. The removal of the enamel organ or metabolic inhibition with iodoacetate caused significant increases in enamel fluoride uptake at both stages of enamel formation. Inhibition with dinitrophenol caused a significant increase only in the maturation phase. Uptake of fluoride in enamel was related to the fluoride concentration in the medium, except in the maturation stage explants, where increasing the medium fluoride concentration from 0.05 ppm to 0.08 ppm did not significantly increase fluoride uptake at any of the three observation times. The findings indicate that the enamel organ exists as a diffusion-limiting membrane to the movement of fluoride from the extracellular fluid compartment to the developing enamel.
REFERENCES
- Bawden, J.W. and Crenshaw, M.A. (1984): Effect of Inhibition of Net Calcium Uptake on Net Fluoride Uptake in Developing Rat Molars, J Dent Res 63:642-645.[Abstract/Free Full Text]
- Bawden, J.W.; Crenshaw, M.A.; Takano, Y.; and Hammarstrom, L. (1982a): Ion Transport Through the Enamel Organ: An Update, J Dent Res 61:1552-1554.
- Bawden, J.W. and Deaton, T.G. (1981): In vitro Study of the Cellular Control of 18F Uptake in the Enamel of Developing Rat Molars, Arch Oral Biol 26:487-490.[CrossRef][Medline]
[Order article via Infotrieve]
- Bawden, J.W.; Deaton, T.G.; and Crenshaw, M.A. (1982b): Role of the Enamel Organ in Limiting Fluoride Uptake During the Maturation Phase of Enamel Development, J Dent Res 61:506-509.[Abstract/Free Full Text]
- Bawden, J.W.; McLean, P.; and Deaton, T.G. (1986): Fluoride Uptake at Various Stages of Enamel Development, J Dent Res 65:34-38.[Abstract/Free Full Text]
- Bawden, J.W.; Merritt, D.H.; and Deaton, T.G. (1981): In vitro Study of 45Ca and 32P Uptake in Developing Rat Molar Enamel Using Quantitative Methods, Arch Oral Biol 26:477-482.[CrossRef][Medline]
[Order article via Infotrieve]
- Bawden, J.W. and Wennberg, A. (1977): In vitro Study of Cellular Influence on 45Ca Uptake in Developing Enamel, J Dent Res 56:313-319.[Abstract/Free Full Text]
- Bawden, J.W.; Wennberg, A.; and Hammarstrom, L. (1978): In vivo and in vitro Study of 59Fe Uptake in Developing Rat Molars, Acta Odontol Scand 36:271-277.[CrossRef][Medline]
[Order article via Infotrieve]
- Crisp, F.D.; Deaton, T.G.; and Bawden, J.W. (1979): In vitro Study of Selenium-75 Distribution in Developing Rat Molar Enamel, Caries Res 18:313-318.
- Erlij, D. and Martinez-Palomo, A. (1978): Role of Tight Junctions in Epithelial Function. In: Ion Transport Across Multimembrane Systems, G. Giebisch, Ed., Berlin: Springer-Verlag, pp. 27-52.
- Hammarstrom, L. (1971): Distribution in Developing Rat Enamel of Simultaneously Injected Fluoride and Calcium, Scand J Dent Res 79:369-376.[Medline]
[Order article via Infotrieve]
- Kurahashi, Y.; Nagai, N.; Watanabe, H.; and Yama, K. (1968): Chronological Observations on the Odontogenesis of Rat Molars, Bull Tokyo Dent Coll 9:147-159.[Medline]
[Order article via Infotrieve]
- Morgan, J.F.; Morton, H.S.; and Raymon, C.P. (1950): Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on Synthetic Medium, Proc Soc Exp Biol Med 73:1-8.[CrossRef][Medline]
[Order article via Infotrieve]
- Taves, D.R. (1968): Determination of Submicromolar Concentrations of Fluoride in Biological Samples, Talanta 15:1015-1023.[CrossRef][Medline]
[Order article via Infotrieve]
- Wennberg, A. and Bawden, J.W. (1978): Comparison of 33P with 45Ca Distribution in Developing Rat Molar Enamel in vivo and in vitro, J Dent Res 57:111-117.[Abstract/Free Full Text]
- Whitford, G.M. and Reynolds, K.E. (1979): Plasma and Developing Enamel Fluoride Concentrations During Chronic Acid-Base Disturbances, J Dent Res 58:2058-2065.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 66, No. 8,
1360-1363 (1987)
DOI: 10.1177/00220345870660081501
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