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Abnormal 45Ca Fluxes in Dispersed Submandibular Acini of Rats Treated with Reserpine

Wenner-Gren Institute, University of Stockholm, Sweden

J.R. Martinez

Department of Child Health, University of Mis,souri Sohool of Medicine. Columbia, Missouri 65212

The uptake and efflux of the isotopic tracer ⁴⁵Ca were compared in dispersed submandibular acini of both control rats and rats treated with seven daily doses of reserpine (0.5 mglkg, i.p.). Tracer uptake occurred in a time-dependent manner in both types of acini and reached 8.4 ± 0.2 and 8.0 ± 0.2 pmol/mg protein, respectively, in acini from control and treated animals after 60 min of incubation. Uptake of tracer was 2.35 nmol/mg DNA in control cells and 4 nmol/mg DNA in cells from treated rats at 60 min. ⁴⁵Ca uptake (per mg protein) was enhanced in control acini 48% by 20 µmol/L epinephrine: 38% by 50 µmol/L carbachol; and 23% by 10 µmol/L isoproterenol. A similar order of potency was observed when uptake was expressed per mg DNA. In acini from reserpine-treated rats, ⁴⁵Ca uptake (per mg protein) was increased 53% by epinephrine, 39% by isoproterenol, and only 8% by carbachol. The same enhanced effect of isoproterenol and lack of effect of carbachol were observed when uptake was calculated per mg DNA. In the absence of secretagogue, efflux of ⁴⁵Ca from tracer-pre-loaded acini was larger in acini from reserpine-treated rats (53%) than in control acini (36%). Whether expressed in terms of mg protein or mg DNA, this efflux was increased in control acini 35% by epinephrine, from 25 to 28% by isoproterenol, and 17% by carbachol. In acini of reserpine-treated rats, epinephrine increased ⁴⁵Ca efflux 20%, isoproterenol from 25 to 28%, and carbachol from 14 to 15%. The time course of epinephrine- and isoproterenol-induced efflux was also different from that in control cells. Thus, chronic treatment with reserpine altered uptake and efflux of ⁴⁵Ca in rat submandibular acini. This suggests alterations in secretagogue-sensitive Ca⁺⁺ pools or gating mechanisms and is likely to underlie disturbances in the Ca⁺⁺-mediated events of the stimulus-response coupling mechanism, such as fluid, electrolyte, and protein secretion.

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Journal of Dental Research, Vol. 66, No. 8, 1294-1299 (1987)
DOI: 10.1177/00220345870660080101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Müller, R.M. Articles by Martinez, J.R. Search for Related Content PubMed PubMed Citation Articles by Müller, R.M. Articles by Martinez, J.R. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL CARBACHOL CHLORIDE EPINEPHRINE RESERPINE Social Bookmarking                         What's this?