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Selection for Streptococcus mutans with an Altered Xylitol Transport Capacity in Chronic Xylitol Consumers

Groupe de Recherche en Ecologie Buccale, Ecole de médecine dentaire, Université Laval, Quebec, QC, Canada GIK 7P4

C. Mouton

Groupe de Recherche en Ecologie Buccale, Ecole de médecine dentaire, Université Laval, Quebec, QC, Canada GIK 7P4

The effect of long-term consumption of refined xylitol on the natural populations of S. mutans in the human oral cavity has been investigated. Fifty four S. mutans strains were isolated from adults and children who had been consuming commercial food products containing xylitol for a period of from 1 to 10 years. Twenty isolates were also obtained from control subjects who had never consumed xylitol-containing commercial food products. The inhibitory effect of xylitol on the isolated strains was determined by monitoring growth on glucose in the presence or absence of xylitol. This was used to define the sensitivity of each isolate to xylitol. Phosphoenolpyruvate:sugar phosphotransferase (PEP-PTS) activities were measured by means of the soluble and membrane fractions prepared from strains from both study populations. It was found that 87% of the fresh isolates from xylitol consumers were xylitol-resistant (XR), compared with only 10% of the strains isolated from the control subjects. The X^R strains had low constitutive fructose PTS activity and very low xylitol-phosphorylating capacity. The xylitol-sensitive (X^s) strains, however, had much higher levels of constitutive fructose PTS activity and phosphorylated xylitol 16 times more rapidly than did the X^R strains. Evidence for the phosphorylation of xylitol by a fructose PEP-PTS in the X^s strains was obtained. The growth inhibition by the intracellular accumulation of non-metabolizable toxic xylitol phosphate and its prevention by the presence of fructose are discussed.

REFERENCES

• Assev, S.; Vegarud, G.; and Rlla, G. (1980): Growth Inhibition of Streptococcus mutans Strain OZM 176 by Xylitol, Acta Pathol Microbiol Scand Sect B 88:61-63.[Medline] [Order article via Infotrieve]
• Batzinger, R.P.; Suh-Yun, L.O.; and Beuding, E. (1977): Saccharin and Other Sweeteners: Mutagenic Properties, Science 198:944-946.[Abstract/Free Full Text]
• Chassy, B.M.; Beall, J.R.; Bielawski, R.M.; Porter, E.V.; and Donkersloot, J.A. (1976): Occurrence and Distribution of Sucrose-metabolizing Enzymes in Oral Streptococci, Infect Immun 14:408-415.[Abstract/Free Full Text]
• Coykendall, A.L. (1976): On the Evolution of Streptococcus mutans and Dental Caries. In: Proceedings, Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds. Sp. Suppl, Microbiol Abst 3:703-712.
• Emilson, C.G. (1983): Prevalence of Streptococcus mutans with Different Colonial Morphologies in Human Plaque and Saliva, Scand J Dent Res 91:26-32.[Medline] [Order article via Infotrieve]
• Engelsberg, E.; Anderson, R.L.; Weinberg, R.; Lee, N.; Hoffee, G.; Huttenhauer, G.; and Boyer, H. (1962): L-Arabinose Sensitive, L-Ribulose 5-Phosphate 4-Epimerase-deficient Mutants of Escherichia coli, J Bact 84:137-146.[Abstract/Free Full Text]
• Gauthier, L.; Vadeboncoeur, C.; and Mayrand, D. (1984): Loss of Sensitivity to Xylitol by Streptococcus mutans LG-1, Caries Res 18:289-295.[Medline] [Order article via Infotrieve]
• Gehring, G.F.; Makinen, K.K.; Larmas, M.; and Scheinin, A. (1974): Turku Sugar Studies. IV. An Intermediate Report on the Differentiation of Polysaccharide-forming Streptococci S. mutans, Acta Odontol Scand 32:435-444.[Medline] [Order article via Infotrieve]
• Gold, O.C.; Jordan, H.V.; and Van Houte, J.A. (1973): A Selective Medium for Streptococcus mutans, Arch Oral Biol 18:1357-1364.[CrossRef][Medline] [Order article via Infotrieve]
• Havenaar, R.; Huis In't Veld, J.H.J.; Backer Dirks, O.; and de Stoppelaar, J.D. (1978): Some Bacteriological Aspects of Sugar Substitutes. In: Health and Sugar Substitutes, B. Guggenheim, Ed., Proceedings, ERGOB Conf., Basel: Karger, pp. 192-198.
• Havenaar, R.; Huis In't Veld, J.H.J.; de Stoppelaar, J.D.; and Backer Dirks, O. (1984): Anti-cariogenic and Remineralizing Properties of Xylitol in Combination with Sucrose in Rats Inoculated with Streptococcus mutans, Caries Res 18:269-277.[Medline] [Order article via Infotrieve]
• Kandelman, D.P. and Hefti, A.F. (1986): The Polynesian Xylitol Study: A Collaborative WHO Clinical Field Trial, J Dent Res 65:534, Abst. 30 (Canadian Division).
• Keene, H.J. (1981): History of Dental Caries in Human Populations: The First Million Years. In: Proceedings, Symposium on Animal Models in Cariology, J.M. Tanzer, Sp. Supp., Microbiol Abst, pp. 23-40.
• Knuuttila, M.L.E. and Makinen, K.K. (1975): Effect of Xylitol on the Growth and Metabolism of Streptococcus mutans, Caries Res 9:177-189.[Medline] [Order article via Infotrieve]
• Krishnan, R.; Wilkinson, I.; Joyce, L.; Rofe, A.M.; Bais, R.; Conyers, R.A.J.; and Edwards, J.B. (1980): The Effect of Dietary Xylitol on the Ability of Rat Caecal Flora to Metabolise Xylitol, Austr J Exper Biol Med Sci 58:639-652.
• Leach, S.A.; Agalamanyi, E.A.; and Green, R.M. (1983): Remineralization of the Teeth by Dietary Means. In: Demineralization and Remineralization of the Teeth, S.A. Leach and W.M. Edgar, Eds., Oxford: IRL Press, p. 51.
• Leach, S.A. and Green, R.M. (1980): Effect of Xylitol-supplemented Diets on the Progression and Regression of Fissure Caries in the Albino Rat, Caries Res 14:16-23.[Medline] [Order article via Infotrieve]
• Lengeler, J. (1975): Mutations Affecting Transport of the Hexitols D-Mannitol, D-Glucitol, and Galactitol in Escherichia coli K-12 Isolation and Mapping, J Bact 124:26-38.[Abstract/Free Full Text]
• Loesche, W.J. (1981): Dental Caries a Treatable Infection, Springfield, IL: Charles C. Thomas.
• Loesche, W.J.; Earnest, R.; Grossman, N.S.; and Cor-Pron, R. (1984): The Effect of Chewing Xylitol Gum on the Plaque and Saliva Levels of Streptococcus mutans, J Am Dent Assoc 108:587-592.[Abstract]
• Loesche, W.J. and Syed, S.A. (1973): The Predominant Cultivable Flora of Carious Plaque and Carious Dentine, Caries Res 7:201-208.[Medline] [Order article via Infotrieve]
• Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
• Makinen, K.K. (1976): Possible Mechanisms for the Cariostatic Effect of Xylitol. In: Monosaccharides and Polyalcohols in Nutrition, Therapy and Dietetics, Ritzel, Brubacher, Eds., Bern: Huber, pp. 368-380.
• Makinen, K.K. (1978): Biochemical Principles of the Use of Xylitol in Medicine and Nutrition with Special Consideration of Dental Aspects, Experientia 30 (Suppl):1-160.
• Makinen, K.K. (1985): New Biochemical Aspects of Sweeteners, Int Dent J 35:23-35.[Medline] [Order article via Infotrieve]
• Makinen, K.K. and Scheinin, A. (1982): Xylitol and Dental Caries, Ann Rev Nutr 2:133-150.[Medline] [Order article via Infotrieve]
• Makinen, K.K. and Soderling, E. (1980): A Quantitative Study of Mannitol, Sorbitol, Xylitol and Xylose in Wild Berries and Commercial Fruits, J Food Sci 45:367-371.[CrossRef]
• Makinen, K.K. and Soderling, E. (1984): Solubility of Calcium Salts, Enamel, and Hydroxyapatite in Aqueous Solutions of Simple Carbohydrates, Calcif Tissue Int 36:64-71.[Medline] [Order article via Infotrieve]
• Muhlemann, H.R.; Schmid, R.; Noguchi, T.; Imfeld, T.; and Hirsch, R.S. (1977): Some Dental Effects of Xylitol Under Laboratory and in vivo Conditions, Caries Res 11:263-276.[Medline] [Order article via Infotrieve]
• Scheinin, A. and Banoczy, J. (1985): Xylitol and Caries: the Collaborative WHO Oral Disease Preventive Programme in Hungary, Int Dent J 35:50-57.[Medline] [Order article via Infotrieve]
• Scheinin, A. and Makinen, K.K. (Eds.) (1975): Turku Sugar Studies, Acta Odontol Scand 33 (Suppl.):70:1-350.
• Shklair, I.L. and Keene, H.J. (1974): A Biochemical Scheme for the Separation of the Five Varieties of Streptococcus mutans, Arch Oral Biol 19:1079-1081.[CrossRef][Medline] [Order article via Infotrieve]
• Shklair, I.L. and Keene, H.J. (1976): Biochemical Characterization and Distribution of Streptococcus mutans in Three Diverse Populations. In: Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Spec. Suppl., Microbiol Abstr, Washington: Information Retrieval, Inc., pp. 201-210.
• Shyu, K.-W. and Hsu, M.-Y. (1980): The Cariogenicity of Xylitol, Mannitol, Sorbitol and Sucrose, Proc Natl Sci Counc Roc 4:21-26.
• Toors, F.A. and Herczog, J.I.D. (1978): Acid Production from a Nonsugar Licorice and Different Sugar Substitutes in Streptococcus mutans Monoculture and Pooled Plaque-Saliva Mixtures, Caries Res 12:60-68.[Medline] [Order article via Infotrieve]
• Trahan, L.; Bareil, M.; Gauthier, L.; and Vadeboncoeur, C. (1985): Transport and Phosphorylation of Xylitol by a Fructose Phosphotransferase System in Streptococcus mutans, Caries Res 19:53-63.[Medline] [Order article via Infotrieve]
• Vadeboncoeur, C.; Trahan, L.; Mouton, C.; and Mayrand, D. (1983): Effect of Xylitol on the Growth and Glycolysis of Acidogenic Oral Bacteria, J Dent Res 62:882-884.[Abstract/Free Full Text]
• Washuttl, J.; Riederer, P.; and Bancher, E. (1973): Qualitative and Quantitative Study of Sugar Alcohols in Several Foods, J Food Sci 38:1262-1263.

Journal of Dental Research, Vol. 66, No. 5, 982-988 (1987)
DOI: 10.1177/00220345870660052301


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