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Journal of Dental Research
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HSV-1-infected Oral Epithelial Cells are Targets for Natural Killer Cells

R.A. Lindemann

Section of Oral Diagnosis, Oral Medicine, and Oral Pathology, and the Dental Research Institute, UCLA School of Dentistry, Center for the Health Sciences, Los Angeles, California 90024

S.H. Golub

Departments of Surgery/Oncology and Microbiology and Immunology, UCLA School of Medicine, Center for the Health Sciences, Los Angeles, California 90024

N.-H. Park

Section of Oral Biology and the Dental Research Institute, UCLA School of Dentistry, Center for the Health Sciences, Los Angeles, California 90024

A mechanism of cell-mediated immunity was investigated to determine whether natural killer (NK) cells were able to lyse antigenically-altered epithelial cell targets. Using standard four-hour chromium-release assays, we tested human peripheral blood lymphocytes against autologous untreated epithelial cells and autologous HSV-1-infected epithelial cells and calculated the percentage of lysis. With adherent cell-depleted peripheral blood, only epithelial cells infected with virus were lysed (p = 0. 009). Evidence that NK cells were responsible for the lysis exists because: (1) peripheral blood lymphocytes were able to lyse allogeneic as well as autologous virus-infected cells; (2) when NK cells were depleted with the lysosomotropic drug L-leucine methyl ester, cytotoxicity against infected targets was abrogated; and (3) depletion of NK cells by the monoclonal antibody Leu-11b, plus complement, also eliminated cytotoxicity against virus-infected targets. Additional evidence suggests that lysis of targets does not involve antibody-dependent cellular cytotoxicity. These findings indicate that NK cells have the potential to perform a similar in vivo immunologic role in the oral cavity and initiate cell-mediated lysis of virus-infected epithelial cells.

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Journal of Dental Research, Vol. 66, No. 3, 770-773 (1987)
DOI: 10.1177/00220345870660031301
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R.A. Lindemann, K.T. Miyasaki, and L.E. Wolinsky
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