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Journal of Dental Research
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Substrate Specificity of Fucosyltransferase Purified from Human Parotid Saliva

H. Tamagawa

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

K. Iwakura

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

A. Amano

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

S. Shizukuishi

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

A. Tsunemitsu

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

The purified fucosyltransferase from human parotid saliva was shown to transfer fucose from GDP-fucose onto the oligosaccharide chains containing the Galβl->3GlcNAc or Gal β 1->4GlcNAc/Glc sequences. Competition studies between asialotransferrin and either lacto-N-fucopentaose 1 or 2'-fucosyllactose provided evidence that both the substrates competed for a common enzyme active site. These results suggest that the fucosyltransferase activities for the three acceptors may be catalyzed by the same enzyme.

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Journal of Dental Research, Vol. 66, No. 3, 756-760 (1987)
DOI: 10.1177/00220345870660031001
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H. S. Cameron, D. Szczepaniak, and B. W. Weston
Expression of Human Chromosome 19p alpha(1,3)-Fucosyltransferase Genes in Normal Tissues
J. Biol. Chem., August 25, 1995; 270(34): 20112 - 20122.
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This Article
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