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Effects of Variations in pH and Hypothiocyanite Concentrations on S. mutans Glucose Metabolism

University of Alabama at Birmingham, Birmingham, Alabama 35294

D.C. Baldone

University of Alabama at Birmingham, Birmingham, Alabama 35294

K.M. Pruitt

University of Alabama at Birmingham, Birmingham, Alabama 35294

F. Rahemtulla

University of Alabama at Birmingham, Birmingham, Alabama 35294

Hypothiocyanous acid (HOSCN) and hypothiocyanite (OSCN^-) were generated by the antibody-independent salivary peroxidase (SP) system. The metabolism of Streptococcus mutans NCTC 10449 was examined by uniformly labeled glucose incorporation studies. We found that the SP-system causes a pH-dependent inhibition of ¹⁴C-labeled glucose uptake, and that the effects of HOSCN/OSCN^- are bacteriostatic. The results also showed that, at low pH, bacteria required more time to recover fully from HOSCN/OSCN^- inhibition. When control experiments were performed in the absence of HOSCN/OSCN^-, but the pH was varied, we found a positive correlation between pH and the rate of ¹⁴C-glucose incorporation. The results also showed that pH did not affect the maximum incorporation of ¹⁴C-glucose, demonstrating that S. mutans can adapt to pH changes in the environment. Based on the data obtained, we postulate that the antibody-independent SP system plays an important role in the regulation of the metabolism of oral streptococci.

REFERENCES

• Appelbaum, B.; Golub, E.; Holt, S.C.; and Rosan, B. (1979): In vitro Studies of Dental Plaque Formation: Adsorption of Oral Streptococci to Hydroxyapatite, Infect Immun 25:717-728.[Abstract/Free Full Text]
• Aune, T.M. and Thomas, E.L. (1977): Accumulation of Hypothiocyanite Ion During Peroxidase-catalyzed Oxidation of Thiocyanate Ion, Eur J Biochem 80:209-214.[CrossRef][Medline] [Order article via Infotrieve]
• Carlsson, J.; Iwami, Y.: and Yamada, T. (1983): Hydrogen Peroxide Excretion by Oral Streptococci and Effect of Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, Infect Immun 40:70-80.[Abstract/Free Full Text]
• Cole, M.F.; Hsu, S.D.; Baum, B.J.; Bowen, W.H.; Sierra, L.I.; Aguirre, M.; and Gillespie, C. (1981): Specific and Non-specific Immune Factors in Dental Plaque Fluid and Saliva from Young and Old Populations, Infect Immun 31: 998-1002.[Abstract/Free Full Text]
• Hamada, S. and Slade, H.D. (1980): Biology, Immunology, and Cariogenicity of Streptococcus mutans, Microbiol Rev 44:331-384.[Free Full Text]
• Hamilton, I.R. (1986): Growth, Metabolism and Acid Production by Streptococcus mutans. In: Molecular Microbiology and Immunology of Streptococcus mutans, S. Hamada, S.M. Michalek, H. Kiyono, L. Menaker, and J.R. McGhee, Eds., Amsterdam: Elsevier Science Publishers, pp. 144-155.
• Harper, D.S. and Loesche, W.J. (1983): Effect of pH upon Sucrose and Glucose Catabolism by the Various Genogroups of Streptococcus mutans, J Dent Res 62:526-531.[Abstract/Free Full Text]
• Hoogendoorn, H. (1976): The Inhibitory Action of the Lactoperoxidase System on Streptococcus mutans and Other Microorganisms. In: Microbial Aspects of Dental Caries, H.S. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Washington: Information Retrieval, Inc., pp. 353-357.
• Hoogendoorn, H.; Piessens, J.P.; Scholtes, W.; and Stoddard, L.A. (1977): Hypothiocyanite Ion: The Inhibitor Formed by the System Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, Caries Res 11:77-84.[Medline] [Order article via Infotrieve]
• Kersten, H.W.; Moorer, W.R.; and Wever, R. (1981): Thiocyanate as a Cofactor in Myeloperoxidase Activity Against Streptococcus mutans, J Dent Res 60:831-837.[Abstract/Free Full Text]
• Kraus, F.W.; Nickerson, J.F.; Perry, W.I.; and Walker, A.P. (1957): Peroxide and Peroxidogenic Bacteria in Human Saliva, J Bacteriol 73:727-735.[Free Full Text]
• Mansson-Rahemtulla, B.; Rahemtulla, F.; Pruitt, K.M.; and Harrington, P.G. (1985): Biochemical and Immunological Properties of Peroxidase from Human Parotid Saliva. In: Protides of the Biological Fluids, Vol. 32, H. Peeters, Ed., Oxford: Pergamon Press, Ltd., pp. 119-124.
• Mansson-Rahemtulla, B.; Baldone, D.C.; Pruitt, K.M.; and Rahemtulla, F. (1986): Specific Assays for Peroxidases in Human Saliva, Arch Oral Biol 31:661-668.[CrossRef][Medline] [Order article via Infotrieve]
• Mickelson, M.M. (1977): Glucose Transport in Streptococcus agalactiae and its Inhibition by Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, J Bacteriol 132:541-548.[Abstract/Free Full Text]
• Moller, A.J.R. (1966): Microbiological Examination of Root Canals and Periapical Tissues of Human Teeth, Scand Dent J 74:12-380.
• Pruitt, K.M.; Demuth, R.E.; and Turner, J.E., Jr. (1979): Practical Application of Generic Growth Theory and the Significance of the Growth Curve Parameters, Growth 43:19-35.[Medline] [Order article via Infotrieve]
• Pruitt, K.M.; Mansson-Rahemtulla, B.; and Tenovuo, J. (1983): Detection of the Hypothiocyanite (OSCN-) Ion in Human Parotid Saliva and the Effect of pH on OSCN- Generation in the Salivary Peroxidase Antimicrobial System, Arch Oral Biol 28:517-525.[CrossRef][Medline] [Order article via Infotrieve]
• Pruitt, K.M. and Reiter, B. (1985): Biochemistry of Peroxidase System: Antimicrobial Effects. In: The Lactoperoxidase System, K.M. Pruitt and J.O. Tenovuo, Eds., New York: Marcel Dekker, Inc., pp. 143-178.
• Pruitt, K.M.; Tenovuo, J.; Mansson-Rahemtulla, B.; Harrington, P.; and Baldone, D.C. (1986): Is Thiocyanate Peroxidation at Equilibrium in vivo?, Biochim Biophys Acta 870:385-391.[CrossRef][Medline] [Order article via Infotrieve]
• Ralston, M. (1985): Derivative-free Nonlinear Regression. In: BMDP Statistical Software, W.J. Dixon, Ed., Berkeley: Univ. of California Press, pp. 305-324.
• Roberts, K.R.; Linder, L.; and Sund, M.-L. (1981): Glucose Incorporation by Human Dental Plaque Bacteria, Caries Res 15:492-500.[Medline] [Order article via Infotrieve]
• Shindler, J.S.; Childs, R.E.; and Bardsley, W.G. (1976): Peroxidase from Human Cervical Mucus: The Isolation and Characterization, Eur J Biochem 65:325-331.[Medline] [Order article via Infotrieve]
• Tenovuo, J. and Anttonen, T. (1980): Peroxidase-catalyzed Hypothiocyanite Production in Human Salivary Sediment in Relation to Oral Health, Caries Res 14:269-275.[Medline] [Order article via Infotrieve]
• Thomas, E.L. and Aune, T.M. (1978): Lactoperoxidase, Peroxide, Thiocyanate ,Antimicrobial System: Correlation of Sulfhydryl Oxidation with Antimicrobial Action, Infect Immun 20:456-463.
• Thomas, E.L. (1981): Lactoperoxidase-catalyzed Oxidation of Thiocyanate: Equilibria Between Oxidized Forms of Thiocyanate, Biochem 20:3273-3280.[CrossRef][Medline] [Order article via Infotrieve]
• Thomas, E.L.; Pera, K.A.; Smith, K.W.; and Chwang, A.K. (1983): Inhibition of Streptococcus mutans by the Lactoperoxidase Antimicrobial System, Infect Immun 39:767-778.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 66, No. 2, 486-491 (1987)
DOI: 10.1177/00220345870660021701


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