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A Cross-reactivity Study of Whole Genomic DNA Probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis
M.N. Strzempko
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
S.L. Simon
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
C.K. French
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
J.A. Lippke
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
F.F. Raia
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
E.D. Savitt
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
K.K. Vaccaro
BioTechnica Diagnostics, Inc., 61 Moulton Street, Cambridge, Massachusetts 02138
In this study, we evaluated the sensitivity and specificity of whole genomic DNA probes for the periodontal pathogens Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis. By means of these probes, DNA hybridizations were performed against other organisms found in the oral cavity and organisms previously determined to be genetically similar. All three probes were sensitive to 103 cells for their respective organism. The H. actinomycetemcomitans probe cross-reacted with several haemophilus strains, Wolinella, and Campylobacter, indicating that H. actinomycetemcomitans-specific sequences would have to be identified and cloned for accurate detection of this organism in heterogeneous patient samples. Only very low levels of cross-reactivity were observed between the B. intermedius probe and representative black-pigmented Bacteroides. This low level of cross-reactivity did not interfere with the accurate identification of B. intermedius in sample evaluations. The B. gingivalis probe showed no cross-reactivity. Whole genomic probes will be used for the detection of B. intermedius and B. gingivalis in patient samples.
REFERENCES
- French, C.K.; Savitt, E.D.; Simon, S.L.; Eklund, S.M.; Chen, M.C.; Klotz, L.C.; and Vaccaro K.K. ( 1986): DNA Probe Detection of Periodontal Pathogens, Oral Microbiol Immunol I: 58-62.
- Holdeman, L.V. and Moore, W.E.C. (1974): Genus I. Bacteroides Castellani and Chalmers 1919, 959. In: Bergey's Manual of Determinative Bacteriology, 8th ed., R.E. Buchanan and N.E. Gibbons, Eds., Baltimore: The Williams and Wilkins Co., pp. 385-404.
- Holdeman, L.V. and Moore, W.E.C. (1982): Description of Bacteroides loeschii sp. nov. and Emendation of the Descriptions of Bacteroides melaninogenicus (Oliver and Wherry) Roy and Kelly 1939 and Bacteroides denticola Shah and Collins 1981, Int J System Bacteriol 32:399-409.[Abstract/Free Full Text]
- Johnson, J.L. and Holdeman, L.V. (1983): Bacteroides intermedius comb. nov. and Descriptions of Bacteroides levii sp. nov., Int J System Bacteriol 33: 15-25.[Abstract/Free Full Text]
- Maniatis, T.; Fritsch, E.F.; and Sambrook, J. (1982): Molecular Cloning (A Laboratory Manual), Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, p.327.
- Marmur, J. and Doty, P. (1962); Determination of the Base Pair Composition of Deoxyribonucleic Acid from its Thermal Denaturation, J Molec Biol 5: 109-122.[Medline]
[Order article via Infotrieve]
- Potts, T.V.; Zambon, J.J.; and Genco, R.J. (1985): Reassignment of Actinobacillus actinomycetemcomitans to the genus Haemophilus as Haemophilus actinomycetemcomitans comb. nov., Int J System Bacteriol 35: 337-341.[Abstract/Free Full Text]
- Rigby, P.W.; Dieckmann, M.S.; Rhodes, C.L.; and Berg, P. (1977): Labelling Deoxyribonucleic Acid to High Specific Activity in vitro by Nick Translation with DNA Polymerase I, J Molec Biol 113: 237-251.[CrossRef][Medline]
[Order article via Infotrieve]
- Slots, J.; Bragd, L.; Wikstrom, M.; and Dahlén, G. (1986): The Occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in Destructive Periodontol Disease in Adults, J Clin Periodontol 13: 570-577.[CrossRef][Medline]
[Order article via Infotrieve]
- Slots, J. and Rosling, B.G. (1983): Suppression of the Periodontopathic Microflora in Juvenile Periodontitis by Systemic Tetracycline, J Clin Periodontol 10: 468-486.
- Tanner, A.C.R.; Listgarten, M.A.; Ebersole, J.L.; and Strzempko, M.N. (1986): Bacteroides forsythus sp. nov., a Slow-growing, Fusiform Bacteroides sp. from the Human Oral Cavity, Int J System Bacteriol 36: 213-221.[Abstract/Free Full Text]
- Van Steenbergen, T.J.M.; Van Winkelhoff, A.J.; Mayrand, D.; Grenier, D.; and De Graaff, J. (1984): Bacteroides endodontalis sp. nov., an Asaccharolytic Black Pigmented Bacteroides Species from Infected Dental Root Canals, Int J System Bacteriol 34: 118-120.[Abstract/Free Full Text]
- Zambon, J.J.; Christersson, L.S.; and Slots, J. (1983): Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Prevalence in Patient Groups and Distribution of Biotypes and Serotypes within Families, J Periodontol 54: 707-711.[Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 66, No. 10,
1543-1546 (1987)
DOI: 10.1177/00220345870660100601
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J. Wahlfors, J.H. Meurman, P. Vaisanen, P. Alakuijala, A. Korhonen, H. Torkko, and J. Janne
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