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Proteolytic Activity of Bacteria Isolated from the Oral Cavities of BALB/c Mice Toward Salivary Proteins
C. Dumas
Departement de biochimie, Faculté des sciences et de génie, et Groupe de Recherche en Ecologie Buccale (GREB), Ecole de médecine dentaire, Université Laval, Quebec, QC, Canada G1K 7P4
A. Champagne
Departement de biochimie, Faculté des sciences et de génie, et Groupe de Recherche en Ecologie Buccale (GREB), Ecole de médecine dentaire, Université Laval, Quebec, QC, Canada G1K 7P4
M.C. Lavoie
Departement de biochimie, Faculté des sciences et de génie, et Groupe de Recherche en Ecologie Buccale (GREB), Ecole de médecine dentaire, Université Laval, Quebec, QC, Canada G1K 7P4
The proteolytic activities of 350 bacterial isolates from different sites (saliva, tongue, teeth, and mucosa) of the oral cavities of BALB/c mice were tested against different proteins found in saliva (immunoglobulins A, M, G, albumin, lysozyme, mucin, lactoferrin, and lactoperoxidase), some of which are considered to possess antibacterial activity. The results indicate that: (1) lysozyme, lactoferrin, and lactoperoxidase are hydrolyzed by from 46 to 70% of the indigenous flora of the oral cavities of BALBlc mice; (2) IgA and IgM appeared less sensitive to the proteolytic activities of these strains than did the other proteins tested; (3) the colonization of the oral cavity does not seem to be correlated with the proteolytic activity; and (4) the presence of specific Ig proteases is relatively scarce within this population.
REFERENCES
- Arnold, R.R.; Pruitt, K.M.; Cole, M.F.; Adamson, J.M.; and McGhee, J.R. (1979): Salivary Antibacterial Mechanisms in Immunodeficiency. In: Proceedings, Saliva and Dental Caries, I. Kleinberg, S.A. Ellison, and I.D. Mandel, Sp. Supp. Microbiol Abstracts, New York: Information Retrieval, Inc., pp. 449-462.
- Baird-Parker, A.C. (1975): Staphylococcus. In: Bergey's Manual of Determinative Bacteriology, 8th ed., R.E. Buchanan and N.E. Gibbons, Eds., Baltimore: The Williams & Wilkins Co., pp. 483-489.
- Cole, M.F.; Hsu, S.D.; Baum, B.J.; Bowen, W.H.; Sierra, L.I.; Aquirre, M.; and Gillespie, G. (1981): Specific and Nonspecific Immune Factors in Dental Plaque Fluid and Saliva from Young and Old Populations, Infect Immun 31:998-1002.[Abstract/Free Full Text]
- Cowman, R.A.; Fitzgerald, R.J.; Perella, M.M.; and Cornell, A.H. (1977): Human Saliva as a Nitrogen Source for Oral Streptococci, Caries Res 11:1-8.[Medline]
[Order article via Infotrieve]
- Cowman, R.A.; Scheafer, S.J.; and Fitzgerald, R.J. (1979): Specificity of Utilization of Human Salivary Proteins for Growth by Oral Streptococci, Caries Res 13:181-189.[Medline]
[Order article via Infotrieve]
- Deslauriers, N.; Neron, S.,; and Mourad, W. (1985a): Immunobiology of the Oral Mucosa in the Mouse, Immunol 55:391-397.[Medline]
[Order article via Infotrieve]
- Deslauriers, N.; Trudel, L.; Seguin, J.; and Lavoie, M.C. (1985b): Differential Recognition of Indigenous Oral Microflora by Salivary Immunoglobulins, J Dent Res 64 (Sp Iss):329, Abstr. No. 1391.
- DiPaola, C.; Herrera, M.S.; and Mandel, I.D. (1984): Host Proteins in Dental Plaques in Caries-resistant versus Caries-susceptible Human Groups, Arch Oral Biol 29:353-355.[CrossRef][Medline]
[Order article via Infotrieve]
- Hsu, S.D. and Cole, M.F. (1985): Structural Integrity of Host Defense Factors in Dental Plaque, Infect Immun 50:398-402.[Abstract/Free Full Text]
- Iacono, V.J.; Byrnes, T.P.; Crawford, I.T.; Grossbard, B.L.; Pollock, J.J.; and Mackay, B.J. (1985): Lysozyme-mediated De-chaining of Streptococcus mutans and its Antibacterial Significance in an Acidic Environment, J Dent Res 64:48-53.[Abstract/Free Full Text]
- Kilian, M. (1981): Degradation of Immunoglobulins Al, A2 and G by Suspected Principal Periodontal Pathogens, Infect Immun 34:757-765.[Abstract/Free Full Text]
- Kilian, M.; Thomsen, B.; Petersen, T.E.; and Bleeg, H.S. (1983): Occurrence and Nature of Bacterial IgA Proteases, Ann NY Acad Sci 409:612-624.[Medline]
[Order article via Infotrieve]
- Mandel, I.D. (1976): Salivary Products in Plaque and Saliva in Relation to Caries. In: Proceedings, Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Sp. Supp. Microbiol Abstracts, New York: Information Retrieval, Inc., pp. 859-866.
- Mandel, I.D. and Ellison, S.A. (1981): Naturally Occurring Defense Mechanisms in Saliva. In: Proceedings, Animal Model in Cariology, J.M. Tanzer, Ed., Sp. Supp. Microbiol Abstracts, New York: Information Retrieval, Inc., pp. 367-380.
- Marsh, P.D. and Martin, M.V. (1984): Oral Microbiology, 2nd ed. Don Mills, Ont.: Thomas Nelson and Sons Ltd., 120 pp.
- Mayrand, D. and Grenier, D. (1984): Immunoglobulin G Proteases in Oral Black-pigmented Bacteroides, J Dent Res 63 (Sp Iss):250, Abstr. No. 712.
- McGhee, J.R.; Michalek, S.M.; and Ghanta, V.K. (1975): Rat Immunoglobulins in Serum and Secretions: Purification of Rat IgM, IgA and IgG and their Quantitation in Serum, Colostrum, Milk and Saliva, Immunochemistry 12:817.
- Navia, J.M. (1977): Animal Models in Dental Research, Birmingham, AL: The University of Alabama Press, Chap. 12, pp. 225-254.
- Plaut, A.G. (1983): The IgAl Proteases of Pathogenic Bacteria, Ann Rev Microbiol 37:603-622.[CrossRef][Medline]
[Order article via Infotrieve]
- Rousseaux, J.; Rousseaux-Prevost, R.; Bazin, H.; and Biserte, G. (1983): Proteolysis of Rat IgG Subclasses by Staphylococcus aureus V8 Proteinase, Biochim Biopsy.; Acta 748:205-212.
- Sundqvist, G.; Carlsson, J.; Herrmann, B.; and Tarn-Vik, A. (1985): Degradation of Human Immunoglobulins G and M and Complement Factors C3 and C5 by Black-pigmented Bacteroides, J Med Microbiol 19:85-94.[Abstract/Free Full Text]
- Taubman, M.A. (1974): Immunoglobulins of Human Dental Plaque, Arch Oral Biol 19:439-446.[CrossRef][Medline]
[Order article via Infotrieve]
- Taubman, M.A.; Smith, D.J.; and Murray, R. (1978): Immunoglobulin Susceptibility to Proteolytic Effects of Human Dental Plaque Extracts, Arch Oral Biol 23:949-955.[Medline]
[Order article via Infotrieve]
- Trudel, L.; St-Amand, L.; Bareil, M.; Cardinal, P.; and Lavoie, M.C. (1986): Bacteriology of the Oral Cavity of the BALB/c Mice, Can J Microbiol (accepted for publication).
- Wikstrom, M.B. (1983): Detection of Microbial Proteolytic Activity by a Cultivation Plate Assay in which Different Proteins Adsorbed to a Hydrophobic Surface are used as Substrates, Appl Environ Microbiol 45:393-400.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 66, No. 1,
62-64 (1987)
DOI: 10.1177/00220345870660011301
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